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Theses

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2001 - 20023
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Subject: Aquaculture × End date: 2002 × Start date: 2001 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Biomodulation of non-specific immune response in the tiger shrimp Penaeus monodon fabricus with spirulina iccorporated diet
    (Department of Aquaculture, College of Fisheries, Panangad, 2002) Jadhav vishal Shripatrao; KAU; Devika, Pillai
    Black tiger shrimp; Penaeus monodon is an important species cultured all over the world. However, disease outbreaks have caused senous economic losses in several countries. For successful farming of shrimps disease prevention is a prime necessity The present study was designed to assess the immunostimulating potential of Spirulina and to see its effect on growth as well as colour of P. monodon juveniles. In this study, juveniles of P. monodon were used for evaluating the effect of different levels of Spirulina on growth, food conversion ratio, food conversion efficiency, survival as well as prophylactic potency of Spirulina evaluated by proPhenoloxidase activity, antibacterial assay, haemagglutinin assay, phagocytosis and challenge study Effect of Spirulina on colour of shrimps was also studied. Five experimental diets designed as T I to T 5 were prepared by using clam meat, Spirulina, rice bran, cod liver oil and vitamin- mineral mix. The total protein content of all diets were kept near to 40% and Spirulina was incorporated at 0%, 10%, 20%, 30% and 40% in diets by replacing clam meat. Completely randomized design with five treatments each having four replications was used for analyzing the results. In each tank ten numbers of Penaeus monodon juveniles were kept. Feeding was done for a period of 35 days. The test animals fed with diet containing Spirulina up to 30% incorporation showed better growth, food conversion ratio and food conversion efficiency. But survival during experiment was not affected by the addition of Spirulina. Spirulina incorporated treatments showed higher phenoloxidase activity than control. Antibacterial activity was also observed in the treatments incorporated with Spirulina, while in control no antibacterial activity was observed. Result of agglutinin activity was not 99 clear as control also showed agglutination in undiluted haemolymph sample and partial agglutination in 1:2 dilution of haemolymph. Spirulina incorporated treatments showed agglutination up to 1:4 dilution of haemolymph. But in 1:4 dilution only partial agglutination was observed. Increased adherence of haemocyte to yeast cells was observed indicating higher phagocytic activity in the treatment incorporated with Spirulina, while adherence of haemocyte was rarely observed in control. On challenge with Vibrio parahaemolyticus, shrimps treated with Spirulina incorporated diet showed delayed mortality as compared to control. Spirulina treated shrimps showed darker colouration than control.
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    ThesisItemOpen Access
    Effect of cuttlefish liver lipid on the growth of macrobrachium rosenbergii (de Man) juveniles
    (Department of Aquaculture, College of Fisheries, Panangad, 2001) Shyla, G; KAU; Mathew, P M
    Giant freshwater prawn is an important species suitable to culture in both fresh and low saline waters. For successful farming of freshwater prawns, nutritionally balanced cheap diet is a prime necessity. The present study was designed to develop a nutritionally balanced feed for freshwater prawn by using cuttlefish liver as a cheap source of lipid. In this study juveniles of Macrobrachium rosenbergii were used for evaluating the effect of various levels of lipid on growth, food conversion ratio, survival rate, protein utilization and body composition. For this six experimental diets designated as T 1 to T 6 were prepared by using clam meat, groundnut oil cake, wheat bran, tapioca powder, vitamin mineral mixture and cellulose powder. Test diets (T2 to T6) were prepared by adding different levels (l to 5%) of cuttlefish liver lipid with an increment of 1 % by replacing equal amount of cellulose powder. Total lipid content of the diets T\, T2, T3, T4, r, and T6 were 7.53%, 8.61%, 9.85%, 10.73%,12.01% and 13.41%, respectively. Completely randomized design with six treatments each having three replications was used for analyzing the results. Ten numbers of juveniles were randomly distributed in each tank Feeding w<..;c done for a period of 60 days. Test animals fed with a diet containing 2% of cuttlefish liver lipid with a total lipid content of 9.85% showed better growth, food conversion efficiency and protein utilization. But survival rate was not affected by the addition of cuttlefish liver lipid. The protein content of body of test animals was maximum for the treatment, T3 having 2% cuttlefish liver lipid. Lipid and moisture contents of the body were at desirable levels for this treatment. Hence, it is found that, the diet containing 2% of cuttlefish liver lipid with a total lipid of9.85% is good for M rosenbergii.
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    ThesisItemOpen Access
    Growth response of labeo rohita (hamilton) fry to soyabean meal based diets
    (Department of Aquaculture, College of Fisheries,Panangad, 2001) Varsha R, Bhatkar; KAU; Syama, S
    Soyabean meal was evaluated as a partial/total substitute for fish meal in diet for Labeo rohita by incorporating at inclusion levels of 10%, 20% and 30%, respectively. Fish meal based diet served as the control. Diet containing 30% of soyabean meal (30%SM) was devoid of fish meal. The overall protein content of the diets was 30%. The study was conducted for a period of 126 days in circular cement cisterns of 350 litre capacity; feeding being done at the rate 5% of the body weight Best growth of rohu was attained on 20% SM diet, followed by lOOiO SM, control and 30% SM. The 30% SM diet was the most stable and the control diet showed the least stability. Fish fed 30% SM diet recorded the lowest average weight on termination of the experiment which was also reflected in the FCE and nutrient digestibility. The inclusion level of soyabean meal was found to influence the carcass composition and enzyme profile. Survival was not affected by soyabean meal incorporation; production was found related to growth rather than survival. Organoleptic quality of fish was not affected adversely by soyabean meal incorporation in the diet.