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Open Access
Evaluation of different protein sources in the formulated feed for macrobrachium Rosenbergii (De Man) Juveniles
(Department of Aquaculture, College of Fisheries,Panangad, 1989) Anilkumar, P; KAU; Thampy, D M
The nutritional quality of five locally available sources of protein such as clam meat, squid waste, squilla shrimp head waste and silkworm pupae was evaluated in the feed for the giant freshwater prawn, macrobrachium rosenbergii, with a view to develop a feed which is cost effective. The feeds were prepared using these locally available ingredients along with groundnut oil cake, wheat bran,tapioca flour, supplevite (a commercially available vitamin- mineral mixture) and sardine oil and maize oil mixture. The protein sources tested were not found to be grossly deficient in their nutritional quality as good survival rates of over 90% were obtained when they were used as the main protein sources in the feed of juvenile prawn. Highest growth rate was obtained for clam and squid based diets, intermediate growth response with squilla and shrimp head based diets and the lowest growth among the various sources was given by silkworm pupae diet. Feed conversion efficiency was higher for clam and squid based diets, while intermediate feed efficiency was noted for squilla and shrimp head meal diets. Lowest feed efficiency was observed for silkworm pupae diet. Protein efficiency ratio showed a similar pattern. In the second experiment, substitution with carbohydrate for protein was found to be promising from an economic point of view. It was found that lowering of protein level from 35 to 30% could be achieved by increasing the carbohydrate level from 20 to 30% without affecting the growth , survival and feed efficiency. Similarly, protein level could be lowered from 30 to 25% by increasing carbohydrate by 10% from 20% level. This suggest that a 5% reduction of costly protein could be achieved by including 10% more of carbohydrate which will be able to reduce the cost of the feed with out sacrificing the nutritional quality of the formulated feed.
Open Access
Effect of eyestalk ablation on growth and reproduction of macrobrachium equidens (Dana)
(Department of Aquaculture, College of Fisheries, Panangad, 1994) Bijulal, P S; KAU; Thampy, D M
The objective of the present study is to evaluate the effect of eyestalk ablation on survival, growth, food conversion and reproduction of adult Macrobrachiumequidens. In the first experiment conducted for 35 days, unilateral eyestalk ablation, bilateral ablation and control treatments were employed at four level of salinities viz 5, 10, 15 and 20 ppt. Complete mortality in bilateral ablation and survival rates of 40.625 % and 68.75% for unilateral and control treatments were recorded at the end of the experiment, the values ranging significantly. The four salinity levels applied did not significantly affect survival. The difference in growth between ablated and unablatedtreatments was insignificant in terms of percentage gain, specific growth rate and average daily percentage gain in terms of length and weight. Salinity levels also showed insignificant difference in growth. Food conversion rate was significantly better in ablated treatment. The different salinity levels tried showed significant difference with regard to food conversion, being better at 20 ppt. In the second experiment conducted for 60 days on females, eyestalk ablation was found to enhance ovarian. In the second experiment conducted for 60 days on females, eyestalk ablation was found to enhance ovarian development in terms of the number of spawns per female. Fecundity, absolute fecundity, and incubation period were not significantly affected although a marginal increase in absolute fecundity could be observed . In the experiments conducted on males it was found that growth rate and cheliped development were significantly better in ablated ones. The number of spines in the appendix masculine, length and biomass of the androgenic gland and width of vas deference, did not show significant variation, although a marginal increase in the biomass of the androgenic gland and the number of spines in appendix masculine was observed in ablated treatment. The different eyestalk ablation experiments have shown that in females destalking does not stimulate growth but there is better response for reproduction, whereas in males there is better growth and only marginal response to reproduction.
Open Access
Conditions for oxygen-packed transportation of penaeus indicus seed
(Department of Aquaculture, College of Fisheries, Panangad, 1994) Geetha Rani Mani, V; KAU; Jayasree Vadhyar, K
The effect of four levels of packing density, (200PL/1, 300PL/1, 400PL/1 and 500PL/1), three levels of salinity (20ppt, 25ppt and 30ppt) and two levels of temperature (30_+ 1°C and 23 + 2°C) on the oxygen consumption rate of Penaeus indicus post-larvae (PL20) was investigated in a closed type respirometer. The dissolved oxygen in the test chamber decreased with the oxygen consumption of the animals. Oxygen consumption was found to vary with ambient oxygen levels at the different packing densities. Among the three salinities, and two temperatures, the lowest rate of oxygen consumption was recorded at 25ppt and 23 +_ 2°C. The effect of these factors on the duration and survival of transportation of the shrimp seed in specially designed hard plastic containers fitted with facilities for oxygen packing under uniform pressure (0.2 Kg/cm2) showed that oxygen packing in hypothermal conditions could help in increasing duration and survival. Salinity of 20-25ppt was found to give longer duration of survival. With increase in packing density, there was considerable reduction in the duration and survival of transportation of the seed. Cannibalism was observed as the major cause of mortality and it could be reduced by lowering of temperature. 200PL/1 could be transported with 100% survival within 6.5-8.5h at ambient temperature of 30+- 1°C under the afore-mentioned type of oxygen-packing. By lowering the temperature to 23 +-_ 2°C it was possible to safely transport with 100% survival the same numbers for more than 20h. Corresponding duration at 500PL/1 with 100% survival was 1-1.5h at ambient temperature and 4-5h at lowered temperature. To reduce cannibalism at ambient temperatures, hollow plastic translucent habitat material was incorporated into the oxygen-packed jars. This experiment was conducted at 25ppt salinity at different packing densities of 200PL/1, 300PL/1, 400PL/1 and 500PL/1. Relatively longer duration and higher survival was observed only at higher packing densities of 400PL/1 and 500PL/1 with the introduction of the habitat material. Water quality parameters in the experimental jars were analysed initially and finally at 70% survival rate. The reduction in dissolved oxygen levels in the oxygen-packed jars was well above the lethal limits even at the highest packing density of 500PL/1 at 70% survival. The decrease in dissolved oxygen levels and increase in ammonia-N and free carbon dioxide, differed with the duration of packing, temperature and packing density.
Open Access
Detritus of plant origin as a food source for Penaeus indicua H. Milne Edwards
(Department of Aquaculture, College of Fisheries, Panangad, 1992) Unnikrishnan, R; KAU; Thampy, D M
The objective of the present study was to evaluate the feasibility of using plant detritus as an exclusive food source for the juvenile of Penaeus indicus and also to find out the level of its in corporation in a standard diet for the prawn in place of animal protein sources. When four plant detritus were simultaneously presented to p.indicus juvenile of size 25-32 mm and 110-150 mg, they exhibited best preference for detritus from Rhizophora apiculata, followed by chromolaena odorta, Pistia stratiotes and paddy detritus. Two better preferred plant detritus sources viz. R.apiculata and C.odorata, were subjected to bulk digestion and the improvement in protein level by way of microbial growth during the process of decay was recorded. Since C.odorata showed a better protein level (Max.av.25.716%) on the fifteenth day than R.apiculata (Max.av.7.528%) on the twentieth day, C.odorata detritus was used for the next two feeding experiments: the first to find whether it could be used as exclusive protein source and the second to find the level at which this could be used to replace animal protein source in a standard diet for P.indicus. The first feeding experiment, for a period of 28 days, to evaluate the feasibility of using plant detritus as sole protein source revealed that an exclusively detritus based diet is not suitable for P.indicus juveniles. The detritus based diet produced lower survival, growth, protein sufficiency ratio, protein digestability and high food conservation ratio when compared to that of control diet prepared with clam meal. The second experiment done with eight diets for a period of 21 days revealed that plant detritus could be used for substituting upto a level of 50% of the animal protein source in a standard prawn diet, without producing any adverse effect on growth and survival. While, a diet containing 10% plant detritus fared even better than a diet which was totally devoid of detrital protein. The growth, food conversation ratio, protein efficiency, apparent digestibility and protein digestibility values obtained for various diets did not vary significantly upto a stage where 50% of the animal protein was substituted with detrital protein, beyond which the values declined. The best values were obtained for the diet FD2 and the lowest for the diet FD8.
Open Access
Protein requirement of the postlarvae and juveniles of Macrobrachium rosenbergii (De Man)
(Department of Aquaculture, College of Fisheries, Panangad, 1992) Sahadevan, P; KAU; Sebastian, M J
Three sets of experiments were conducted with the postlarvae and juveniles of the giant freshwater prawn. Macrobrachiumrosenbergii (De Man). The first experiment was conducted to determine the quantitative protein requirement of the two life stages of the prawn using casein and amino acid based purified diets having graded levels (0, 10, 20, 30, 40 and 50%)of protein. Each experiment was conducted for 40 days and the effect of protein concentration on the survival, growth, food intake, food conversion efficiency, protein digestibility, nitrogen exeretion, nitrogen retension, body carcass composition, efficiency of protein utilization and oxygen – nitrogen ratio was studied. Among the different diets the one with 30% protein produced the best results in the postlarvae and juveniles of the prawn. Using second order polynomial regression analysis and differential calculus methods the protein requirement for maximum growth in the postlarvae and juveniles of the prawn was calculated as 34.5 and 28.5% respectively. In absolute terms these represent 787.9 and 529.0mg protein per 100g body weight of the prawn per day. The economic protein requirement of the postlarvae and juveniles was found to be 27.5 and 23.0% respectively. The maintenance protein requirement of the postlarvae and juveniles was determined to be 17.95 and 18.69mg protein per 100g body weight per day assuming an efficiency of utilization of protein very close to 100%. The metabolic faecal nitrogen excretion was found to be 198 and 151mg N per 100g body weight per day for the postlarvae and juveniles respectively. In the second set of experiments, short term (10 days each) studies were conducted to determine the efficiency of assimilation of certain locally available sources of protein by the postlarvae and juveniles of M. rosenbergii. These experiments were conducted also to help fix the levels of protein to be maintained in the third set of experiments. The protein sources evaluated were casein, prawn meat, clam meat, soybean, ground nut oil cake and black gram. The results of the study indicated that the apparent protein digestibility is not influenced by animal or plant origin of the ingredient. The third set of experiments was conducted to evaluate the effect of substitution of protein of animal origin with that of plant origin in the postlarvae and juveniles of M. rosenbergii. Each experiment was conducted for 40 days and on the basis of data on survival, growth, food intake, food conversion efficiency, protein digestibility, carcass composition, efficiency of protein utilization and oxygen – nitrogen ratio, substitution of 50 and 75% protein of animal origin with that of plant origin was found to result in no significant decline in the overall performance of the postlarvae and juveniles respectively of M. rosenbergii.
Open Access
Evaluation of supplementary feeds and optimum ration for Chanos chanos (Forskal) fry
(Department of Aquaculture, College of Fisheries, Panangad, 1991) Elizabeth, Chrolin; KAU; Susheela, Jose
The present study was conducted in C. chanos fry,to evaluate the efficacy and keeping quality of supplementary feeds compounded from five different protein sources viz., fish meal ( FM ), squid meal (SQM), clam meal (CM), prawnhead waste meal (PHM) and soybean meal (SYM). All the feeds used in the study were isocalorific (3.13 - 3.33 Kcal/g) and isonitrogenous (39.78 - 40.44% crude protein). The quality of feed after four months of storage was satisfactory as there was not much variation in their nutrient contents. The FCR of fish fed on various supplementary feeds for 42 days were 1.74, 2.94, 3.65, 5.32 and 10.9 for CM, SQM, FM, SYM and PHM, Respectively. The PER, protein digestibility co-efficient and 0: N ratio indicate that protein from clam meal is better utilized for growth Purposes than those from others. The PER value ranged from 0.23 (PHM) to 1.45 (CM). The protein digestibility co-efficient, for CM, SQM, FM, SYM and PHM were 97.24,91,86, 86.56, 77.78 and 65.23%, respectively. The 0: N ratio was highest (10.769:1) for fish fed on CM. It seems that C. chanos fry digest lipid from animal protein sources better (digestibility values ranging from 84.17% (PHM) to 98.8% (CM)) than that from plant source SYM (47.49%).The biochemical composition of fish Showed maximum synthesis of protein and deposition of fat in fish fed on CM, followed by SQM, and the least in fish fed on PHM. The evaluation of supplementary feeds based on survival rate, gain in length and weight, and specific growth rate indicate the superior performance of animal protein sources (except prawn head waste meal) to plant source. The highest gain in growth was attained by fish fed on CM, followed by SQM and FM. Performance of fish fed on SYM was not satisfactory, while PHM was a poor feed for C. chanos fry. The optimum food ration of fry is at 5.2% of the body weight when fed with CM, twice a day, at 4-6 ppt salinity.
Open Access
Effects of various growth promoters in the diets of macrobrachium rosenbergii post larvae
(Department of Aquaculture, College of Fisheries, Panangad, 1994) Boby Ignatius; KAU; Susheela Jose
The present study was conducted to evaluate the efficiency of various growth promoters on the growth of Macrobrachium rosenbergii post larvae. The various growth promoters tried were antibiotic oxytetracycline, thyroid hormone thyroxine and the enzyme papain. Each growth promoter was incorporated in a casein based purified diet having a protein content of 36%.In the second experiment, the optimum level of growth promoter which showed the best performance in the first experiment was determined. The growth increment of prawns fed on casein based purified diets incorporated with antibiotic oxytetracyc1ine designated as diet T1 was 43.66mg, it was 35.064 mg for diet T2 - containing thyroid, and 32.172 mg for diet T3- containing papain. The growth increment for the control diet was seen to be 26.72 mg. Specific growth rate of the prawns from different treatments ranged from 1.73 to 2.52; with control diet showing the lowest and T1 the highest. Incorporation of the growth promoters in the feed has markedly reduced the food conversion ratio of the animals. Lowest FCR was obtained for the post larvae fed with oxytetracycline, while the highest was for the control, the range being 2.94 to 4.36. The protein efficiency ratio was highest being 0.9770 for the feeds containing oxytetracycline. The PER values for the control diet T0, diet T2 with thyroid, diet T3 with papain were found to be 0.6566, 0.8541 and 0.7576 respectively. Productive protein values were 8.995 for control diet, 13.4763 for diet with oxytetracycline, 11.7710 for diet with thyroid hormone and 10.4266 for diet with papain enzyme. The average survival rate during the experiment was 69.5%. The animals fed on antibiotic incorporated feed gave maximum survival of 72%, while the lowest rate of 66% was observed for thyroxine incorporated feed (T2). Analysis of the data on the various growth parameters in the experiment reveals that the antibiotic oxytetracycline gives better growth amongst the different growth promoters used. In the experiment II, four different levels of oxytetracycline were tested to find out the optimum level. The result of the study showed that, 10 mg/lOOg of feed is the optimum level of oxytetracycline which give maximum growth in Macrobrachium rosenbergii post larvae as indicated by overall growth , specific growth rate, food conversion ratio and protein efficiency ratio.
Open Access
Effect of low salinities on the growth and survival of Penaeus mondon Fabricus
(Department of Aquaculture, College of Fisheries, Panangad, 1988) Navas, K A; KAU; Sebastian, M J
Growth and survival of P.monodon are influenced by a number of ecological factors (Chakraborti et al. 1986) salinity, being one of the most important of them. Knowledge on the tolerance of this species to low salinity conditions has great implications in its culture especially in extending its culture to low saline fields. In the present study, the postlarvae were subjected to wide fluctuations in salinity ranging from 1 to 20ppt. The influence of gradual acclimation in improving ranges of tolerance of postlarvae was studied. The lowest lethal salinity (LC 50) was determined by step-wise reduction of salinity. Salinity in which more than 50% mortality occurred at the end of 120h was considered lethal. Growth of juvenile in different test salinities ranging from 1 to 20 ppt for a period of 56 days was undertaken. Preliminary tolerance studies indicated that fluctuations in salinity ranging from 4 to 20ppt did not influence the survival of postlarvae significantly (P>0.05). Gradual acclimation extended the range of tolerance. A probit regression line for the relation between the logarithmic salinity level (dose =X) and mortality (Probit response =Y) was found to be Y=5.982023 + 1.332194X. The lowest lethal salinity (LC 50) was 0.5479 ppt with its upper and lower limits being 0.377 and 0.790 ppt respectively. Post larvae exhibited abnormal behaviour and higher rate of cannibalism at suboptimal salinities. Low salinities had a highly significant influence on the growth of juvenile (P<0.001). However, mean values of growth rate between 4 and 15 ppt did not differ significantly (P>0.05) from the control (19.5 + 0.5 ppt). Shrimps reared in different test salinities were subjected to sensory evaluation. Flavour of cooked meat of shrimps differed significantly with variation in salinities (P<0.05). The overall taste panel score was highest at 9.5 + 0.5ppt. The possibility of the influence of salinity on flavor of cultured shrimps is suggested.
Open Access
Standardization of oxygen packing procedure of chanos chanos seed
(Department of Aquaculture, College of Fisheries, Panangad., 1991) Mary Marget, M J; KAU; Jayasree Vadhyar, K
The present study was aimed at finding out the optimum conditioning method of C.chanos seed before packing, their optimum packing density, the effect of salinity, pH and oxygen pressure on their packing and the feasibility of using a sedative, tertiary butyl alcohol and chitosan as an absorbent in removing toxic metabolites accumulated in the oxygen –packed containers. Specially designed air tight plastic containers were used for packing the seed. Of the seed packed after four treatments viz. control, congestion,, gut voiding and gut voiding plus congestion, the fourth treatment was the best with 3.25 fold increase in the duration of 100% survival compared to the control. From packing densities of 20,30,40 and 50 numbers per 50 ml water, a linear relationship between packing density in g/50 ml(X) and time of initial mortality in hours (Y) was established as Y=51.398-20.44X. No significant effect on the duration and survival of the seed was observed at different levels of salinity (0.ppt, 10ppt and 20pp), initial pH (6.5-7.5, 7.5-8.5 and 8.5-9.5) and oxygen pressure (oxygen at atmospheric pressure and at 0.2 kg/cm2 pressure). The use of tertiary butyl alcohol at 3.0 ppm and 3.5 ppm resulted in light and deep sedation of the seed respectively. It also increased the duration of 100% survival by 1.67 and 1.25 fold at the respective doses, compared to the control. The feasibility of using chitosan as an absorbent in oxygen packing of the seed was ruled out.

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