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ThesisItem Open Access Superovulation, Sychronisation of oestrus and embryo transfer in crossbred cows(Department of Animal Reproduction, College of Veterinary and Animal Sciences, Mannuthy, 1992) Suresan Nair, S P; KAU; Neelakanta Iyer, C PThe object of the present investigation was to ascertain the influence of varying doses of FSH and PGF2₻on various parameters of superovulation and embryo collections, with the ultimate aim of fixing the optimum dose of FSH and PGF2 ₻ required for successful superovulation in crossbred cows. Detailed investigation was made in a herd of 32 crossbred cows belonging to the University Livestock Farm, attached to the College of Veterinary and Animal Sciences, Mannuthy, during the period from April 1989 to December, 1991. The animals were randomly divided into 6 groups based on 3 doses of FSH and 2 doses of PGF2 ₻ administered. It was observed that 20 mg of FSH, with 25 mg of PGF2 induced satisfactory superovulation response and 20 mg of FSH with 15 mg of PGF2 ₻ resulted in better embryo quality. Further increase in the dose did not show any improvement in the responses. Right ovary showed more response than the left. Day 11 was found to be ideal for starting superovulation treatment, and day 6 for embryo collection, though statistically no significant influence was noticed by day of collection on superovulation response. Parity and age of donors had no influence on superovulation. Rainy and summer months were found to be better seasons for superovulation treatment than winter. Shorter duration (24 hr) of oestrum was noticed in more number of animals. 20 mg of FSH and 25 mg of PGF2 ₻ (group 3) yielded better cervical dilatation and flushing efficiency. Level of progesterone increased following superovulation treatment and declined 3 days before oestrum and reached the lowest level on day of oestrum. Pregnancy rate achieved was 33.3 per cent, but calving percentage was nil.ThesisItem Open Access Ovarian response to gonadotrophin releasing hormone in non-cyclic goats(Department of Animal Reproduction, College of Veterinary and Animal Sciences, Mannuthy, 1998) Aravinda Ghosh, K N; KAU; Mathai, EThe study was conducted to evaluate the effect of Gonadotropic Releasing Hormone (GnRH) on ovarian activity and its usefulness in restoring normal oestrous cycle in adult non-cyclic goats. A total of 60 healthy Malabari, Malabari x Saanen and Malabari x Alpine does aged one to four years, with a record of one or more kiddings and 45 days post partum belonging to Goat Farm of Kerala Agricultural University, Mannuthy were closely observed for a period of 60 days for the occurrence of oestrus and serum samples were collected at fortnightly intervals for the estimation of macro and micro minerals. Serum progesterone profile in eight cyclic and sixteen non-cyclic does, selected at random, were estimated by Radioimmunoassay using a coat-A-count progesterone kit. Out of eight non-cyclic does treated with potent GnRH anologue (Buserelin) three responded to single dose and one responded to a second dose. The oestrus was exhibited at a mean of 87.000 ± 9.950 h after the administration of GnRH analogue. The mean length of oestrous cycle and duration of oestrus in cyclic does were 20.313 ± 1.553 days and 37.500 ± 7.263 hrs while in GnRH responded does were 12.750 ± 0.830 days and 18.000 ± 4.240 hrs, respectively. The mean serum phosphorus level was found significantly higher in GnRH responded does (5.375 ± 0.205 mg per cent) as compared to cyclic does (4.800 ± 0.260 mg per cent) and non-cyclic does (4.770 ± 0.280 mg per cent). There was significantly higher serum copper level in GnRH responded (1.123 ± 0.089 ppm) and in cyclic does (1.160 ± 0.170 ppm) as compared to non-cyclic controls (0.830 ± 0.110 ppm) . The serum zinc level was significantly higher in non-cyclic (1.510 ± 0.430 ppm) as compared to cyclic (1.180 ± 0.120 ppm) and GnRH responded does (1.155 ± 0.091 ppm). There was no significant difference in the serum calcium, cobalt and manganese level between the three groups. The mean serum progesterone in cyclic does for the two consecutive cycles was 0.304 ± 0.087, 1.294 ± 0.382, 2.531 ± 0.758, 3.619 ± 0.794, 2.456 ± 0.430 and 0.871 ± 0.246 ng/ml and in GnRH responded does was 0.158 ± 0.026, 0.800 ± 0.177, 1.475 ± 0.334, 0.675 ± 0.236, 0.280 ± 0.030 and 0.120 ± 0.021 ng/ml on day one, four, six, ten, 14 and 18 of cycle, respectively. The overall mean serum progesterone during induced cycle in GnRH responded does was 0.535 ± 0.139 ng/ml which was significantly lower as compared to cyclic does (1.848 ± 0.339 ng/ml) but significantly higher as compared to untreated non-cyclic does (0.190 ± 0.106 ng/ml), Detailed biometry studies of pituitary ovary, uterus and cervix of GnRH responded does showed non-significant increase in the size and weight as compared to untreated non-cyclic does. The mean number and size of follicles were found significantly higher in GnRH responded as compared to non-cyclic does. The mean tissue ACP, ALP and LDH in the pituitary, ovary and uterus of GnRH responded does showed non significant increase as compared to untreated non-cyclic does. The mean LDH level in both ovaries together was significantly higher in GnRH responded does as compared to non-cyclic does. The present study confirms that GnRH administration in non-cyclic does has reactivated the ovary by increased follicular growth, maturation and corpus luteum formation. However, the length of induced oestrous cycle, duration and intensity of oestrus was significantly less in GnRH responded does