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Utero-tubal insufflation technique for diagnosis of tubal impatency in cows
(Department of Animal Reproduction, College of Veterinary and Animal Sciences, Mannuthy, 1981) Athman, K V; KAU; Neelakanta Iyer, C P
The objective of the present study was to design an apparatus for utero-tubal insufflation test and to standardize its use in both excised genitalia and in live animals for the diagnosis and treatment of tubal impotency. Perfect obturation of the cervix was obstained using a stainless steel catheter with 22 FR. Neo Foley’s latex catheter sleeved over. Unilateral and bilateral impotency were recorded in 8.70 and 19.25 per cent of the excised genitalia. The incidence of unilateral and bilateral impotency in 37 infertile animals were found to be 2.70 and 13.52 per cent respectively. The mean pressure at which air escaped from both the tubes in excised genitalia was 128.12 mm. Hg. In cows and 148.93 mm. Hg. In heifers. On the contrary in experimental animals the corresponding pressure in cows and heifers were 116.38 mm. Hg. And 127.50 mm. Hg. Respectively. It was observed that a higher pressure was required to open both the tubes in heifers than in cows. Blown up condition of the uterus took place in excised genitalia of cows and heifers between 100-280 mm. Hg. And 100-260 mm. Hg. Respectively. The pressure range for producing “blown up” condition in experimental animal was found to be 120-220 mm. Hg. In cows and 195-200 mm. Hg. In heifers. Since the safety margin was estimated to be 60.00 mm. Hg. In experimental cows, the test is considered to be a safe diagnostic test for tubal impotency. Although uterus might be “blown up” in a very few cases at a pressure lower than the higher pressure for escape of air from normally patent tubes, the test will not endanger the life of the animal. Since 69.56 percentage of cows with bilateral patency of salpinx on testing conceived after insemination, utero-tubal insufflation test could also be exploited for the treatment of temporary tubal blockage.
Open Access
Reproductive pattern and performance of nanny goats in Kerala
(Department of Animal Reproduction, College of Veterinary and Animal Sciences, Mannuthy, 1992) Krishna Kumar, G; KAU; Sudarsanen, V
A study to elucidate the reproductive pattern and performance of nanny goats under the agroclimatic conditions of Kerala conducted on a flock of 154 nanny goats of mixed genotype, Malabari, Saanen x Malabari and Alpine x Malabari, maintained under the All India Co – ordinated Research Project on goats for milk, Kerala Agricultural University, has revealed the following results and conclusions. The method adopted was detection of oestrus twice a day using a vasectomised buck and by clinical examination. On detection, all animals of good reproductive health were bred artificially with freshly collected semen extended in Tris buffered yolk extender and preserved at low temperature. A group of animals were given a second insemination after seven hours of the first insemination for a comparison of the success rate to single and double inseminations during a heat. Following breeding, the duration of gestation, durations of stages of parturition, presentation of kids, incidence of multiple birth and secondary sex ratio were also recorded. Daily record of maximum and minimum temperature for a period of one year of the study was maintained. The period was divided into four quarters almost in agreement with the four natural seasons and the conceptions during each of the quarters were related with their secondary sex ratio and birth weight records to know the influence of the environmental temperature on them. Placental area weight records were also maintained and by applying linear regression equation they have been correlated with the birth weight to establish that the birth weight difference was due to the influence of environmental temperature on placental growth and development. Goats were found to be polyoestrous and 61. 54 per cent came into oestrus during the period from April to September when the day is long. Of the two peak breeding activities noticed the greater one occurred in July and the other during November. Breeding activity was found to be low during the months of January and February. The duration of oestrous cycle was found to be 18 – 23 days but the cycle length varied from 6 to 140 days. 45.8 per cent of the animals had an oestrus duration of less than 18 days. Duration of oestrus varied from 12- 48 h. Cessation of oestrus was noticed in 84.8 per cent of animals by 36 h and in 93.26 per cent of animals by 48 h. Conception percentage to first time single and double inseminations during a heat were 33.93 and 42.85 respectively. Overall conception percentages for the above were 71.42 and 82.14 respectively. Average gestation length was 145.62 ± 0.23 days while with singleton, twin and triplet, the durations were 146.05 ± 144.86 ± 0.32 and 145.25 ± 1.03 days. In twin pregnancies and in pregnancies with male foetus/foetuses in both singleton and homogamous twin, was found to have lesser duration of gestation. Mean durations of the first, second and third stages of labour were 57.47 ± 10.29 , 14.52 ± 1.34 and 128.87 ± 4.84 min respectively. Presentation of kids at the time of birth was 88.9 per cent in anterior and the rest in posterior presentation. Secondary sex ratios of singleton and multiple pregnancies were 52.05 and 54.08 per cent with an overall of 53.22 per cent. No significant variation could be observed in the secondary sex ratio of kids those were conceived between seasons having variable environmental temperature. Mean birth weight of kids born during the three trimesters with mean environmental maximum temperature 39.0, 33.4 and 36.30 C were respectively 1.64, 1.92 and 1.88 kg. Between the trimesters having 39. 00 C and 33.40 C there was a significant increase in birth weight. A definite correlation was found to exist between the placental weight and birth weight and the placental area and birth weight. The following conclusions were derived : 1. There is a reproductive pattern difference as could be seen from the above as 61.54 per cent of the oestruses occurred during the period of long days, contrary to the belief that breeding season of goats is the short days. Peak breeding activity was also noticed during the month of July. An instinctive attempt to reduce the number of births during the months of heavy rain which is not conducive for the survival of the young could be appreciated from the low breeding activity seen during January and February. 2. The duration of oestrous cycle, oestrus, gestation and the first, second and third stages of parturition were found to be in consonance with the already available informations. 3. Two inseminations during a heat was found to improve the conception percentage over single insemination. 4. Incidence of multiple pregnancy was found to be lower in the flock. 5. Secondary sex ratio of the kids born was 53.22 per cent and it confirms well with the reports already available. Presentation of foetus was anterior in 88.9 per cent and the rest of posterior presentation. 6. Conception between periods of variable environmental temperature did not seem to influence the sex ratio, to significantly alter the secondary sex ratio. 7. Environmental temperature at the time of conception was found to significantly influence the birth weight of kids. From the correlation that could be established between placental weight and birth weight and between placental area and birth weight it could be inferred that the environmental temperature effect on birth weight is through its influence on placental development and growth.
Open Access
Observation on gestation and parturition in goat-Capra hircus
(Department of Animal Reproduction, College of Veterinary and Animal Sciences,Mannuthy, 1981) Kuriakose, K K; KAU; Neelakanta Iyer, C P
A systematic study involving the use of 85 does comprising 27 Malabari, 35 Alpine x Malabari and 23 Saanen x Malabari crosses reared and maintained at Mannuthy, Kerala, under All India Co – ordinated Research Project on Goats for Milk was carried out in order to ascertain the gestation length, process of parturition and placental details. In addition 583 breeding particulars gathered from the registers maintained at the Project also utilized for assessing the gestation length and frequency of occurrence of multiple births. The salient observations made and the valid inferences drawn are indicated below. The average gestation length of different genetic groups of goats was found to be 146.66 + 0.53 days. There was no significant variation in the gestation period of does of different genetic groups both in single and multiple births. Parity had no influence on the gestation length in does. The variation in gestation length due to difference in the sex of kid was also not significant. Gestation period was not found to vary between seasons. The frequency of occurrence of single, twin and multiple births was found to be 50.75 per cent, 45.05 per cent and 4.19 per cent respectively. Signs of approaching parturition was assessed based on the degree of manifestation of such symptoms as tumefaction of the vulva, udder engorgement and relaxation of the pelvic ligaments. The symptoms exhibited by the does were only moderate in the beginning but on nearing parturition there was conspicuous increase in the intensity of these changes. Colostrum was present in all the animals two days prior to parturition. Visible flow of cervical mucus was evident in 75 does two days prior to kidding and in all, 24 hours before parturition. There was no significant fluctuation of body temperature of does during prepartum period. The total duration of parturition was 360.91 + 30.85, 358.20 + 16.67 and 395.42 + 40.09 minutes in single births and 359.12 + 26.15, 357.20 + 32.23 and 368.73 + 24.03 minutes in multiple births in Malabari, Alpine x Malabari and Saanen x Malabari goats respectively. The duration of parturition was not variance between genetic groups both in single and multiple births. The weight of kid was positively correlated to the weight of placenta, total number of cotyledone and to the number of large and medium sized cotyledons. There was negative correlation between weight of kid and the number of small sized cotyledons. The number of cotyledons in the gravid horn was significantly higher than that in non gravid horn in all the genetic groups. Weight of the placenta as well as the total number of cotyledone was found to be more in multiple pregnancy than in single pregnancy.
Open Access
Investigation on the pathological conditions in the genitalia of female goats
(Department of Animal Reproduction, College of Veterinary and Animal Sciences, Mannuthy, 1980) Ramachandran, K; KAU; Neelakanta Iyer, C P
A study was undertaken to assess the incidence and nature of pathological conditions affecting the genitalia of female goats. The material for the present study comprised of 950 genitalia, inclusive of 42 gravid ones, collected at random, from the slaughter house, Corporation of Cochin, Ernakulam. The organs which revealed gross lesions and those which were suspected to be affected were subjected to detailed histopathological studies. Pathological lesions of genital organs were observed in 48 (5.053%) genitalia. Thirty two (3.368%) genitalia revealed various pathological changes of the ovaries and pathological changes of the uterus were recorded in20 (2.105%) genitalia. The cervix, vagina and vulva did not reveal any pathological changes in any of the genitalia examined. The following pathological conditions were observed during the course of the present study: Ovarian hypoplasia (0.316%); cystic ovarian degeneration (0.947%); cystic corpus luteum (0.421%); chronic oophoritis (0.211%); multifollicular atresia (0.105%); par ovarian cyst (1.368%); Bursitis (0.842%); salpingitis (0.105%); macerated foetus (1.684%); endometritis (0.211%); cystic glandular hyperplasia (0.105%) and cyst in the endometrium with sub – epithelial haemorrhage (0.105%).
Open Access
Superovulatory response, embryo collection and transfer in crossbred cows
(Department of Animal Reproduction, College of Veterinary and Animal Sciences, Mannuthy, 1996) Unnikrishnan, M P; KAU; Suresan Nair, S P
Out of eleven crossbred cows superovulated with 2000 IU of PMSG on day eleven and 25 mg PGF2 alpha 48 h later, nine animals (81.8%) exhibited oestrum after an average interval of 39.44 ± 2.44 h. Average duration of oestrum was 40.00 ± 2.00 h with 66.7 per cent of them exhibiting intense heat signs and 33.3 per cent exhibiting only moderate heat signs. Average number of corpora lutea and unovulated follicles in both the ovaries put together was 6.67 ± 0.50 and 6.22 ± 0.57 respectively. Ovarian response was more in right ovary than in left ovary, though not statistically significant. Average fluid recovery on flushing was 79.5 per cent which was comparatively low. The average embryo recovery and percentage of embryo recovery were 3.38 ± 0.70 and 53.11 ± 10.18 respectively, which was comparatively lower. The reason for poor recovery of embryo was attributed to poor fluid recovery on flushing. The average number and percentage of transferrable embryo recovered were 1.50 ± 0.53 and 33.34 ± 10.14. Reason for these lower rates were attributed to loss of embryo quality, due to prolonged action of PMSG. Donors of parity over three performed better on superovulation and flushing, than those below three. Animals of age group six to nine years produced more transferrable embryos than cows of age group ten and above. A conception rate of 30 per cent was achieved after transfer of embryos to naturally synchronised recipients. Heifers appeared to be better recipients than cows. Although a high incidence of abortion was encountered, birth of an embryo transfer male calf was also recorded in the study. All the donor cows came into regular oestrous cycle within four months of superovulation treatment.
Open Access
Studies on post- partun oestrum in cross bred cows
(Department of Animal Reproduction, College of Veterinary and Animal Sciences, Mannuthy, 1979) Suresan Nair, S P; KAU; Neelakanta Iyer, C P
The object of the study was to find out the various reproductive parameters that influence the onset of post – partum oestrus in cross bred cows. With this object in view, a detailed investigation was undertaken in a herd of 115 cross bred cows of Jersey x Sindhi and Jersey x Local breeds belonging to the University Livestock Farm of the Kerala Agricultural University, Mannuthy during the period from February 1978 to March, 1979. After parturition, they were grouped into normal and abnormal calvers based on the normalcy of calving. All the cows were stall fed and were under identical conditions of feeding and management. The observations made and inferences drawn are summarised below: There were significant variations in the cessation of lochial discharge, regression of pregnancy corpus luteum, period of uterine and vulval involution, onset of first and second ovulations and the post – partum oestrus interval between normal and abnormal parturitions. Duration of lochial discharge, regression of pregnancy corpus luteum and onset of first ovulation were found to be significantly influenced by the season of calving. Parity of the cow was found to influence the period of lochia, involutions of uterus and also the post – partum oestrus interval. Milk yield of the cow significantly influenced regression of pregnancy corpus luteum and vulval involution. However, the breed of the cow and sex and weight of calf were not found to have any significant effect on the reproductive parameters studied. An incidence of 13.4 per cent of aberrations of oestrum was also observed.
Open Access
Effect of different glycerol concentrations on freezing of buck semen
(Department of Animal Reproduction, College of Veterinary and animal Sciences, Mannuthy, 1995) Prasanth, V; KAU; Mathai, E
With the object of studying the effect of different concentrations of glycerol on post-thaw motility and fertility of frozen buck semen, five Malabari crossbred (Alpine x Malabari) bucks maintained at Artificial Insemination Centre and 75 does in heat, brought to the A. I. centre, College of Veterinary and Animal Sciences, Mannuthy, Thrissur were used. The average volume of semen was 0.71 ± 0.02 mI. Semen volume varied significantly between bucks. The average colour index was creamy, density was DDDD and the mass activity was ++++. The average pH of semen was 6.85 ± 0.01. The average sperm motility percentage was 85.83 ± 1.05. Motility percentage varied significantly between bucks. The average sperm concentration was 2842.33 ± 153.93 millions/mI. Highly significant difference in sperm concentration was noted between bucks. The average live sperm percentage was 91.03 ± 0.56. There was highly significant difference in live sperm percentage between bucks. Significantly higher percentage of sperm motility was noted in spermatozoa with seminal plasma than in spermatozoa without seminal plasma at 60 minutes of incubation. Slight increase in motility percentage was noted for spermatozoa with and without seminal plasma at 10 minutes of incubation. From 10 minutes to 60 minutes motility was gradually decreasing. Between bucks there was highly significant difference in sperm motility of incubated spermatozoa. Live sperm percentage was significantly higher for spermatozoa without seminal plasma at all time periods of cold shock. There was a rapid reduction in live sperm percentage at five minutes of cold shock. The live sperm percentages between bucks were significant at different time periods except at 10 minutes and 30 minutes of cold shock. The average time taken for reduction of methylene blue by spermatozoa with and without seminal plasma were 173.16 ± 8.77seconds and 197.00 ± 9.97 seconds respectively. Significant difference was noted in methylene blue reduction time between bucks. Average percentage sperm motility after washing was 76.71 ± 0.79. Maximum percentage motility after glycerolisation was obtained in six per cent glycerolated semen (67.85 ± 1.39). There was highly significant reduction in motility percentage after glycerolisation. Maximum post-thaw motility was obtained in six per cent glycerolated extender (42.00 ± 1.84). Highly significant reduction in motility percentage was noted after freezing. The conception percentage and kidding percentage were 47 .36 and 43. 85 respectively. Average gestation length was 149.85 ± 4.45 days. The number of kids per kidding averaged 1.7. Percentage of male and female kidsborn were 51.16 and 48.83 respectively. From this study it could be inferred that Tris extender with six per cent glycerol was superior to Tris extender with four per cent, five per cent or seven per cent glycerol for better post-thaw motility and fertility of frozen buck semen.
Open Access
Synchronisation of oestrus super ovulation and embryo collection in goats
(Department of Animal Reproduction, College of Veterinary and animal Sciences, Mannuthy, 1994) Benjamin, E D; KAU; Nair, M S
With the object of evolving effective methods for standardising techniques for synchronisation of oestrus superovulation and collection of embryos 18 healthy goats were selected from the goat farm attached to the College of Veterinary and Animal Sciences Mannuthy and randomly divided into three different groups with six animals in each Animals m the first group were given two doses of 10 mg PGF2cl H days apart and those in the second group were given 12 5 mg progesterone injection daily for 16 days Six animals in the third group were not given any treatment and kept as control The respective treatments were repeated in group I and II after a period of sixty days and superovulation in group I was carried out with eCG injection 1000 IU given intramuscularly on the day previous to the second dose of PGF2a In group II superovulation was done by intramuscular injection of 1000 IU eCG given on the 15th day of progesterone treatment All the animals in the above groups and animals in the control group were inseminated with good quality buck semen four to six hours after the onset of oestrus Embryos were collected surgically m all the eighteen goats by flushing the fallopian tube towards the fimbria after conducting laparotomy of the inseminated goats on the left flank All the animals in group I came to oestrus 57 8 ± 5 65 h after the second injection of PGF2a and the duration of oestrus was 48 ± 8 76 h In group II 83 33% were m oestrus at an interval of 101 6 + 6 11 h after the last progesterone injection and the duration of oestrus was 28 ± 1 41 h The results of administration of eCG in the second treatment regime with PGF2a in group I revealed that all animals in this group evinced oestrus at a mean interval of 50 3 ± 10 86 h after the second injection of PGF2a and the mean duration of oestrus was 44 ± 4h The total number of ovulation points on both the ovaries in this group were 8 4 ± 1 94 with 4 ± 1 30 and 4 4 ± 0 748 for the right and left ovaries respectively The total number of unruptured follicles on both the ovaries was 5 33 ± 1 64 The animals in group II after administration of eCG and progesterone evinced oestrus 72+9 06 h after the last progesterone injection with the duration of oestrus as 38 3 ± 4 46 h The total number of ovulation points on both the ovaries was 12 8 ± 1 4 and the values were 7 5 ± 2 31 for the right and 5 3 ± 2 04 for the left ovary The total number of unruptured follicles on both the ovaries was 3 2 + 1 579 The results of embryo collection in animals in group I revealed that the average number of embryos collected from both the ovaries was 4 8 ± 0 97 with 2 2 ± 0 66 for the right and 2 6 ± 0 39 for the left ovary The total number of embryos collected from all the animals in both the ovaries was 24 (57 14%) of which 17 (70 83%) were transferrable
Open Access
Prostaglandin therapy for post-partum clinical endometritis
(Department of Animal Reproduction, College of Veterinary and animal Sciences, Mannuthy, 1993) Jacob, T C; KAU; Madhavan, E
The object of the investigation was to evaluate the therapeutic values of alpha# for evolving a non antibiotic alternative for the treatment of post partum clinical endometritis. For this, 42 cross-bred cows, belonging to University Livestock Farm, Mannuthy, having post partum clinical endometritis were divided into four groups. Group I consisted of 10 animals which were watched for their natural oestrus and inseminated twice at 24 hours interval. In group II, 11 animals were observed for their natural oestrus and inseminated twice at 24 hours interval and were given post insemination intrauterine antibiotic treatment 24 hours later based on antibiotic sensitivity test. Eleven animals in group III were subjected to induction of oestrus by administration of PGF2 alpha (Lutalyse) 25 mg intramuscular 8-12 days of their cycle and inseminated twice at 24 hours, at the induced oestrus. Group IV consisted of 10 animals subjected to induction of oestrus as in group III and inseminated twice at 24 hours interval and were given post insemination intrauterine antibiotic therapy based on sensitivity tests, 24 hours later. The observations made and inferences drawn are given below. The interval from the administration of PGF2 alpha to the onset of oestrus ranged from 4.8-120 hours (mean 61.81 hours) and 36 to 72 hours (mean 54.0 hours) in group III and IV, respectively. The mean duration of oestrus was 21.6 hours, 23.36 hours, 28.36 hours and 31.60 hours in the four groups respectively. The duration of oestrus showed significant variation between groups I and IV (f = 2-8910) and between groups II and IV (t* = 2.6445). The percentage of intense, medium and weak oestrus was 66.66, 23.80 and 9.52 per cent respectively in natural oestrus and 66.66, 19.04 and 14.28 in induced oestrus respectively. The difference in the intensity of oestrus between natural and induced oestrus was not significantly different, although, a slightly high incidence of weak oestrus was observed, when oestrus was induced with Lutalyse. Physical changes of the reproductive tract' like oedema of the vulva, congestion of vulval mucosa and sliminess did not show any variation between the natural oestrus and induced oestrus. The percentage of animals showing purulent discharge, discharge with flakes and cloudy discharge showed a marked reduction when treated with PGF2 alpha alone and a combination of PGF2 alpha and antibiotics. Similarly the percentage of animals showing clear discharge increased enormously by above treatments. The bacterial organisms isolated from the uterine discharges were citrobacter spp. 23.84 per cent, Bacillus spp.. 23.80 per cent, S. aureus .14.28 per cent, Pseudomonas 14.28 per cent, Corynebacterium spp. 9.52 per cent, Coagulase negative staphylococci, 9.52 per cent and the yeast Candida guilliermondii 4.76 per cent. Gentamicin was the most sensitive antibiotic for most of the organisms isolated, followed by chloramphenicol, oxytetracycline and sulphadiazine. Penicillin was the most resistant followed by streptomycin and nitrofurantoin. Significant difference in the overall conception rate was observed between different groups; the overall conception rate was significantly higher in group IV than in group I and II (t1 = 4.8341 between groups I & IV and t' = 2.9186 betweengroups II & IV). Significantly higher conception rate was observed in group III than group I also (t ' = 5.5886). The number of inseminations required per conception was lowest in group III and highest in group I. Thus, it appeared that PGF2 alpha in combination with antibiotic was beneficial in the treatment of clinical endometritis. But since the number of inseminations required for conception was lower in group III than group IV and because, there is no significant difference in the overall conception rate, between these two groups, it could be inferred that administration of antibiotics along with P G F 2 alpha did not have any added advantage. Furthermore, considering the harmful effects of administration of antibiotics, it may be stated that PGF2 alpha alone would be beneficial in the treatment of post partum clinical endometritis and can be recommended as the drug of choice

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