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ThesisItem Open Access Effect of different freezing rates on canine spermatozoa(Department of Animal Reproduction, College of Veterinary and Animal Sciences, Mannuthy, 2000) Geetha, R; KAU; Sreekumaran, TThe objective of the study was to find out the effect of different freezing rates on post thaw motility, livability and acrosomal damage of dog spermatozoa. A total of 36 ejaculates of good quality collected from SIX Dachshund dogs by digital manipulation were processed for freezing in Tris citric acid fructose egg yolk diluent containing four per cent glycerol. The processed semen samples were subjected to three different freezing protocols such as 4cm height above the liquid nitrogen level for 10 minutes (Fast freezing), Scm for 15 minutes (Moderate freezing) and 12cm for 20 minutes (Slow freezing). The mean volume of sperm rich fractions was 0.6S±0.03ml. The colour and consistency of sperm rich fractions were thin milky. The mean density of sperm rich fraction was DD(D) and mean pH was 6.63±O.02. The mean concentration of sperm rich fraction was 221±7.36 millions per ml and the average initial motility was found to be 75±O.93 per cent. The mean percentage of live sperm count, sperm abnormalities and acrosomal damage of spermatozoa was Sl.17±O.73, 5.23±O.29 and 2.32±O.25 respectively. Significant (Pabnormalities and acrosomal damage of spermatozoa was found between dogs. The average percentage of motility, live sperm count, sperm abnormalities and acrosomal damage of spermatozoa was 70.41± 1.22, 75.63±O.65, 7.28±0.43 and 5.34±O.31 after dilution, 58.75±1.34, 63.60±O.89, 10.04±O.32 and 10.13±0.41 after chilling and 47.78±1.59, 50.65±1.31, 11.79±O.36 and 16.20±O.57 after equilibration period respectively. There was significant (Preduction in sperm motility and livability and increase in sperm abnormalities and acrosomal damage of spermatozoa after dilution, chilling and equilibration period. Significant (Pwas found between dogs for the above parameters. The percentage of post thaw motility of spermatozoa was significantly (Pwhen compared to moderate (25.83±1.66) and slow (24.44±1.27) freezing rates. There was significantly (Pof live sperms and lower percentage of sperm abnormalities in fast freezing rate than in moderate and slow freezing rates. Eventhough the percentage of acrosomal damage was not statistically ( significant among fast, moderate and slow freezing rates, lower percentage of acrosomal damage was recorded in fast freezing rate. From this study it could be inferred that fast freezing in which the straws were frozen at to 4cm height above the liquid nitrogen level for 10 minutes was superior to moderate (8cm for 15 minutes) and slow (12 cm for 20 minutes) freezing rates.