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ThesisItem Open Access Influence of different levels of energy on growth performance of crossbred pigs(Department of Animal Nutrition, College of Veterinary and Animal Sciences, Mannuthy, 2001) Rekha, P; KAU; George, MathenAn experiment was conducted to assess •the influence of different levels of energy on the growth performance of crossbred (Large White Yorkshire x Desi) pigs. Fifteen male (castrated) and fifteen female weaned crossbred piglets with an average body weight of 12.9 kg were divided into three equal groups as uniformly as possible with regard to age, sex and body weight. The three groups of piglets were maintained on three rations with 16 per cent crude protein but differing in their energy content, viz., 2800 kcal (T1), 3000 kcal CT2) and 3200 kcal (TI) of digestible energy (DE) per kg. The average daily gains recorded for the three groups T1, T2 and TI were 262.9, 302.0 and 362.8 g respectively. The cumulative feed conversion efficiencies were 6.0, 5.2 and 4.1 for the groups T1, T2 and TI respectively. The values recorded for TI were higher (P<0.01) than those for T1 and T2. The digestibility coefficients of nutrients except that of crude fibre and crude protein were found to improve with increase in the energy content of the rations. Study of the carcass traits revealed that the body weight at slaughter and dressed weight without head improved as the energy content of the rations increased. However, dressing percentage, carcass length and back fat thickness were not significantly influenced by the energy content of the ration. The cost of feed per kg weight gain of animals was significantly lower (P<0.01) for the dietary treatment T3 compared to that of T2 and Tl, the values being Rs.49.90, 43.30 and 34.10 for Tl, T2 and T3 respectively. The above results indicate that crossbred pigs require 3200 kcal of DE/kg of the ration for better growth performance provided the crude protein level is maintained at 16 per cent.ThesisItem Open Access Microbial degradation of mimosine in goats(Department of Animal Nutrition, College of Veterinary and Animal Sciences,Mannuthy, 1995) Prabhakaran, P; KAU; Devasia, P AAn investigation was carried out to find out the extent of in vitro microbial degradation of pure mimosine (T1) and that of immature leaves (T2), mature leaves (T3), tender stems (T4) and seeds (T5) of L. leucocephala using strained rumen liquor obtained from three rumen fistulated Saanen – Malabari crossbred goats maintained under standard conditions of feeding and management. The proximate chemical composition and mimosine content of different edible parts of leucaena during the months of May, June and July were determined. While immature leaves and seeds had higher crude protein content, seeds had higher crude fat, tender stems had higher crude fibre and mature leaves had higher ash content compared to other edible parts of subabul. The average mimosine concentrations of T2, T3, T4 and T5 were 12.11 + 0.05, 4.89 + 0.02, 3.90 + 0.04 and 10.70 + 0.08 per cent respectively during May; 11.66 + 0.06, 5.23 + 0.03, 3.62 + 0.03 and 10.44 + 0.05 per cent respectively during June and 9.96 + 0.05, 4.92 + 0.03, 3.73 + 0.02 and 9.51 + 0.04 per cent respectively during July on a dry matter basis. The average mimosine concentrations of strained goat rumen liquor incubated with 37.50 mg/100 ml of added mimosine in pure form or as immature leaves, mature leaves, tender stems and seeds showed significant reduction at every 12 hr intervals from 0 to 48 hr of incubation, the final average concentrations being 23.98 + 0.37, 23.14 + 0.37, 22.20 + 0.28, 23.12 + 0.52, 23.35 + 0.37 mg/100 ml of SRL. The percentage of in vitro degradation in respect of T1, T2, T3, T4 and T5 increased significantly at every 12 hr intervals of incubation from 0 to 48 hr, even though the degradation was incomplete with all treatments, the average percentage degradation at 48 hr of incubation being 31.69 + 1.02, 34.49 + 1.18, 37.12 + 0.99, 34.54 + 1.50 and 33.41 + 1.03 respectively. The overall average rate of disappearance of mimosine in µg.ml-1 . h-1 in respect of T1, T2, T3, T4 and T5 for the entire period of 48 hr of incubation were 2.33, 2.54, 2.74, 2.54 and 2.44 respectively with highest rates during 0 to 12 hr, lower rates during 24 to 36 hr and least rates during 36 to 48 hr. The production of ammonia and VFA coincided with the active degradation of mimosine, there being faster degradation upto 12 hr of incubation with highest concentrations of ammonia and VFA at 12 hr of incubation. The overall results indicated that the rumen microorganisms of crossbred goats degrade mimosine to DPH, ammonia and VFA and that mimosine does not inhibit the microbial activity, even though the possible role of leucaena endogenous enzymes in the partial degradation of mimosine recorded in the present study cannot be ruled out.ThesisItem Open Access Effect of dried spleen as growth stimulator in kid rations(Department of Animal Nutrition, College of Veterinary and Animal Sciences,Mannuthy, 1994) Shyama, K; KAU; James, C SAn investigation was carried out to assess the effect of dried spleen as a growth promotant in kid rations. Twenty four female Malabari kids with an average body weight of 8.5 kg were distributed randomly and as uniformly as possible into four groups (groups 1, 11, 111 and IV) of six animals each, with regard to age and weight. The four dietary treatments A, B, C and D were allotted to the kids in the groups 1, 11, 111 and IV respectively, ration A with a concentrate mixture containing 16 per cent crude protein supplemented with dried buffalo spleen at the rate of 0.1 per cent, ration B forming the control diet at 16 per cent protein level without incorporation of spleen, ration C with a concentrate mixture containing 12 per cent crude protein supplemented with dried buffalo spleen at the rate of 0.1 per cent, ration D forming the control diet at 12 per cent protein level. Jack leaves formed the sole source of roughage to the animals. The experimental duration was 120 days. The results indicated that incorporation of dried spleen in the ration enhanced the growth performance of animals, especially in animals maintained on low level of protein, as evidenced by the cumulative and average daily weight gain registered in that descending order being 7.52 kg and 62.64 g, 7.4 kg and 61.67 g, 6.88 kg and 57.36 g and 5.00 kg and 41.67 g for the animals maintained on rations C, A, B and D respectively. The efficiency of feed and protein utilisation also exhibited the same trend. It was also noticed that, a cost of production per kilogram gain of Rs.32.58 and 26.14 were registered for animals maintained on spleen incorporated rations (rations A and C) as against Rs.35.08 and 38.63 respectively for the animals maintained on corresponding control groups (ration B and D) indicating a better cost efficiency in spleen incorporated groups, especially in animals maintained on low level of crude protein in the ration. The haematological studies reveal that R.B.C., W. B. C., haemoglobin, plasma protein, plasma calcium, phosphorus and magnesium were within the normal range prescribed for the species. The animals maintained on ration C showed a significant increase (P < 0.01) in plasma calcium level when compared to that of the control. Digestibility studies reveal no significant difference in digestibility of dry matter and nitrogen free extract between the four groups. Animals maintained on the spleen incorporated rations (groups 1 and 111) showed better digestibility (P < 0.01) of crude protein and ether extract than the respective control groups (groups 11 and IV). Incorporation of dried spleen could not bringforth any influence on fibre digestion. The results of the balance experiment showed better retention of nitrogen, calcium, phosphorus and magnesium in the animals maintained on spleen incorporated diets. The higher retention of these parameters could be substantiated by the comparatively lower faecal and urinary excretion of these, in animals maintained on spleen incorporated rations (rations A and C). An overall assessment of the results, indicated that incorporation of dried buffalo spleen in the ration of goats, at the rate of 0.1 per cent, certainly augment the nutrient utilisation by increasing the digestibility and retention, and can be recommended as a harmless natural growth promotant with economic benefit.ThesisItem Open Access Influence of feed enzymes on nutrient availability and production performance of laying hens(Department of Animal Nutrition, College of Veterinary and Animal Sciences, Mannuthy, 1995) Satyamoorthy, B; KAU; Maggie Menachery, DThe influence of feed enzymes, viz., cellulose or protease supplemented at levels of 0.06 or 0.02 per cent respectively, on nutrient availability and on production performance was evaluated in a standard and a less dense layer ration using one hundred IWN strain of Single Comb White Leghorn pullets of 20 weeks of age for an experimental period of 20 weeks. A standard layer ration was formulated as per BIS, 1993. With 18 per cent crude protein and 2600 kcal of ME per kg of feed and a less dense layer ration with 16 per cent crude protein and 2500 kcal of ME per kg of feed. A few feed ingredients such as jowar, deoiled rice bran, wheat bran and undecorticated sunflower cake were included in both rations. A numerical improvement was noticed in percentage hen – housed and hen – day egg production in enzyme treated groups, however, the increase in egg number was not statistically significant. Egg weight and egg quality characteristics such as yolk and albumen indexes, Haugh unit scores and shell thickness were not affected by enzyme treatment. A positive influence of the enzymes on feed intake by birds was observed in as much as the feed intake with less dense ration supplemented with enzymes was not different statistically from those with the control ration. There was positive enzyme effect on feed per egg in treatment groups on standard layer ration with both enzymes and in less dense ration with protease. Feed efficiency for egg production (egg number) was significantly higher in birds fed standard layer ration with cellulose and protease than those on other treatments (P < 0.05). There was improvement in digestibility of crude protein and utilisation of energy, even though the differences noted were not significant statistically. The enzyme treatment of rations reduced moisture content of excreta. The microbial load on the surface of eggs was comparatively low in enzyme treated groups. The intestinal viscosity was reduced with enzyme addition in feed mixtures. The cost – benefit analysis indicated that but for the prohibitive enzyme cost, the feed enzymes were nutritionally beneficial. Based on the present investigation it is concluded that there is tremendous scope for enzymes in feed industry, especially at the present context of feed shortages and the emphasis for the utilisation of alternative feed resources in poultry rations.ThesisItem Open Access Nutritive evaluation of squilla (oratosquilla nepa) meal in broiler chicken diets(Department of Animal Nutrition, College of Veterinary and Animal Sciences, Mannuthy, 2000) Kanakasabai, P; KAU; Kunjikutty, NAn experiment was designed and conducted to assess the nutritive value of squilla (Oratosquilla nepa) meal in broiler chicken diet as an animal protein source replacing fish meal protein at three levels viz. zero, 50 and 100 per cent with supplementation of lysine and methionine at 100 per cent level of replacement. One hundred and eighty one – day old straight – run commercial broiler chicks were divided into four groups each with three replicates of fifteen birds and were allotted to four dietary treatments viz. T1 containing 10 per cent unsalted dried fish (control), T2 in which 50 per cent crude protein from dried fish was replaced with squilla meal protein, T3 in which 100 per cent crude protein from dried fish was replaced with squilla meal protein and T4 in which T3 was supplemented with adequate lysine and methionine as per BIS (1992). The rations were made isoproteimic and iso – caloric. The chicks were reared under standard managemental conditions for a period of eight weeks. The birds fed on 100 per cent squilla meal protein dist in replacement of fish meal protein attained significantly lower body weight gain )P < 0.01) compared to those in the control ( T1) and 50 per cent squilla meal protein (T2) group at six and eight weeks. Lysine and methionine supplementation in the 100 per cent squilla meal protein diet improved the weight gain of birds to a level comparable to those on 50 per cent squilla meal protein diet. Feed intake and protein of birds on various dietary treatments at different ages did not differ significantly (P > 0.05). But the feed conversion efficiency of birds maintained on control diet was significantly higher (P < 0.05) at six weeks and eight weeks (P < 0.01) than those on 100 per cent squilla meal protein diet (2.437 vs 2.93). Protein efficiency ratio of birds differed significantly between different treatment groups and the values correlate well with the body weight gain at different ages. Slaughter studies revealed no significant difference (P > 0.05) between the different groups in dressed yield, giblet yield and length of duodenum. However, the abdominal fat was significantly higher (P < 0.01) in control group of birds, while the ready – t0 – cook yield was significantly less (P < 0.05) in 100 per cent squilla meal protein group. The apparent metabolisable energy content of different rations and daily protein retention of birds showed no significant difference between the different dietary treatments. The cost of production per kilogram of live weight gain of birds maintained on the four dietary treatments T1, T2, T3 and T4 were Rs. 26.42, 26.24, 27.25 and 27.79 respectively. Though there was a slight decrease in the cost of production of birds due to incorporation of squilla meal in place of unsalted dried fish at 50 per cent replacement, the difference was insignificant. However, 100 percent replacement of fish meal protein with squilla meal protein and amino acid supplementation to the same increased the cost of production (P < 0.01) with no corresponding increase in performance. From the results obtained in the present study it was inferred that squilla meal can be effectively included in broiler chicken diets to replace 50 per cent of fish meal protein without affecting growth, feed conversion efficiency and the cost of production.ThesisItem Open Access Energy supplementation on production performance of cows under field condition(Department of Animal Nutrition, College of Veterinary and Animal Sciences, Mannuthy, 2008) Ruby, Augustian; KAU; Mercy, A DA study was conducted to assess the effect of energy supplementation in the form of maize and rumen protected fat in early lactating cows under the field condition. Thirty, early lactating cross bred cows were selected and divided into three groups uniformly with regard to days in milk, the average days in milk being 40 days. The animals were randomly allotted to the dietary treatments T1, T2 and T3. The concentrate mix made in the semi liquid form and paddy straw formed the T1 ration. The animals of T2 and T3 were supplemented with 1 kg ground maize and 100 g of rumen protected fat respectively, over and above the control ration. The animals maintained on the three dietary treatments T1, T2 and T3 showed an average milk production of 15.38, 14.58 and 13.01 kg respectively during the 90 day period of the experiment. Statistical analysis showed no significant difference (P>0.05) in milk yield between the three treatment groups. The average daily DMI and DMI/100 kg body weight remained similar for all the three groups. The TDN intake was up to the requirement in animals maintained on T3 ration while T1 and T2 rations could not meet the requirements of the animals of the respective groups. The DCP intake was optimum from the three rations. The energy supplementation did not affect milk composition such as percentage of total solids, milk fat, solids not fat (SNF) and milk protein, and milk urea nitrogen (MUN). The 4 per cent FCM yield, total yield of milk fat and protein remained unaffected by energy supplementation. Animals in all the three groups showed similar blood parameters such as haemoglobin, plasma glucose, plasma urea nitrogen and plasma Ca and P. The digestibility of EE, DM, NDF and ADF was higher for the T3 ration than that of T1 and T2. The digestibility of crude protein (CP) was higher for T2 than T1 and T3 rations. Animals maintained on T3 ration showed earlier post partum heat. The cost of feed for producing 1 kg milk was higher for the dietary treatments T2 and T3. An overall assessment of the results obtained in the present study showed that energy supplementation in the form of either maize or rumen protected fat could not influence dry matter intake, milk yield and 4 per cent FCM yield. There was no change in the composition of milk and blood parameters studied, among the experimental animals. However, energy supplementation in the form of protected fat could meet the energy requirement of early lactating animals, improved the digestibility of EE, DM, NDF and ADF and positively influenced the first post partum heat.ThesisItem Open Access Organic chromium supplementation on growth of cross bred pigs(Department of Animal Nutrition, College of Veterinary and Animal Sciences, Mannuthy, 2007) Ann Nisa, Thomas; KAU; Mercy, A DAn investigation was carried out with the objective to determine the effect of chromium as chromium propionate on the growth of cross bred pigs and to evaluate the economics of organic chromium supplementation in the ration of cross bred pigs. Twenty four weaned piglets (12 male and 12 female) with an average body weight of 18 kg were used as experimental animals. The piglets were randomly divided into two groups with six replicates of two piglets in each group and were randomly allotted to two dietary treatments T1 (control diet) and T2 (control + 200 ppb chromium). The pigs were weighed at the beginning of the experiment and later on at fortnightly intervals. Blood samples were collected in the beginning and at the end of the experiment for the analysis of various biochemical parameters. Digestion trial was conducted at the end of the experiment to determine the digestibility coefficient of the nutrients of the experimental diets. Animals in the two dietary treatments showed similar (p>0.05) body weight gain, average daily body weight gain and feed conversion efficiency. There was a numerical increase in dry matter and crude protein digestibility of Cr supplemented ration. There was no significant difference (p>0.05) between the animals in the two groups for all the blood parameters studied except phosphorus and cholesterol, which were significantly lower (p<0.05) for T2 group. There was non-significant decrease in triglycerides and increase in HDL cholesterol level. The overall critical evaluation of the results obtained in the present study indicate that though supplementation of chromium propionate did not affect the growth or feed conversion efficiency of growing (Large White Yorkshire X Desi) cross bred pigs, it can be beneficially used to improve the lipid profile of the cross bred pigs.ThesisItem Open Access Effect of dietary potassium diformate on growth performance in large white yorkshire pigs(Department of Animal Nutrition, College of Veterinary and Animal Sciences,Mannuthy, 2003) Suresh, M; KAU; Viswanathan, T VA study was carried out to assess the influence of potassium diformate on growth performance, digestibility of nutrients and carcass quality in Large White Yorkshire pigs. Thirty weaned male piglings with an average live weight of 13.0 kg were divided into three equal groups as uniform as possible with regard to age and body weight. Pigs were maintained on three experimental diets viz., T j - control standard grower (18 per cent crude protein and 3150 kcal/kg ME) and finisher ration (15.5 per cent crude protein and 3100 kcal/kg ME), T2 - control diet supplemented with 0.75 per cent potassium diformate and T3 - control diet supplemented with 1.5 per cent potassium diformate. The average daily gain of 465,494 and 519 g were recorded for the groups Tj , T2 and T3, respectively. There was no significant difference (P>0.05) between the supplemented group and the group fed control diet. However, there was a trend for a higher growth rate of 6.2 per cent and 11.7 per cent, reported for T2 and T 3 over those fed control diet. The average cumulative feed conversion efficiency were 3.97, 3.74 and 3.55 for the groups Tj, T2 and T3, respectively. Addition of potassium diformate at 0.75 and 1.5 per cent level in the diet of growing-finishing pigs had a higher (Pthose fed control diet. Ther,e was no significant difference (P>0.05) between the supplemented groups. However, there was a trend for a higher feed efficiency for those fed 1.5 per cent potassium diformate supplemented diet. The digestibility coefficients for dry matter, ether extract, crude protein and nitrogen free extract were significantly higher (Ppotassium diformate at both the levels (0.75 and 1.5 per cent). There was no significant difference between the supplemented groups. Dressing percentage, carcass length, back fat thickness, loin eye area and others were not significantly influenced (P>0.05) by the inclusion of potassium diformate at different levels. The costs of feed per kg live weight gain of pigs were Rs.32.82, 32.05 and 31.50, respectively for T1, T2 and T3. The above results indicate that potassium diformate can be economically incorporated at l.5 per cent level in the diet of Large White Yorkshire pigs.ThesisItem Open Access Effect of citric acid and microbial phytase on phosphorus utilization and growth in broiler chicken(Department of Animal Nutrition, College of Veterinary and Animal Sciences,Mannuthy, 2003) Hariharan, T; KAU; Gangadevi, PAn investigation spread over a period of eight weeks was carried out to study the effect of citric acid and microbial phytase (Natuphos®-SOOOG) on nutrient utilization and growth performance in broiler chicken. One hundred and ninety two day-old broiler chicks (Ven cob) were divided into four identical groups having four replicates in each group with 12 birds in each replicate and allotted randomly into four dietary treatments viz., T1, T2, T3 and T4. The treatments consisted of a standard broiler ration (SBR) with O.S per cent available P (T1), low available P broiler ration having 0.3 per cent available P (LAPBR) and 3.0 per cent citric acid (T2), LAPBR supplemented with 700 U of phytase/kg feed (T3) and LAPBR with 1.5 per cent citric acid and 3S0 U of phytase/kg feed (T4). All the rations were formulated as per BIS specifications except in the level of available P. Effect on body weight, weight gain, feed efficiency, protein efficiency, DM retention, nitrogen balance, mineral availability, serum and tibial mineral contents, processing yields and livability of birds were the criteria employed for evaluation. Body weight and weight gain of the experimental birds were significantly influenced by the dietary treatments. Maximum weight and weight gain were recorded in T4 followed by T2, T3 and Tl in the descending order, indicating the positive and synergistic effects of citric acid and phytase on phosphorus utilization and growth. Cumulative feed intake of experimental birds was significantly (Pgroups over the control, the highest feed intake being noticed in T4 followed by T2, T3 and T1. Cumulative FCR did not differ significantly (P>O.OS) between treatments. However, comparatively better feed efficiency was recorded in citric acid and phytase groups (T2, T3 and T4) at sixth week and comparable values at eighth week with the control group (Tl). Cumulative PER also showed similar trend in eight week with significantly high (Psignificantly, better DM retention, nitrogen balance, per cent retention and reduced nitrogen excretion were noticed in T2, T3 and T4 than Tl. Availability of P, Mg, Zn and Mn were enhanced (P>0.05) in T2, T3 and T4 by citric acid and phytase addition compared to Tl group on SBR. Mg and Mn availability were significantly influenced by dietary treatments with highest availability of Mg and Mn noticed in T3 (P<0.05) followed by T4, T2 and Tl. The excretion of P was significantly reduced (P<0.05) in T2, T3 and T4 on citric acid and for phytase supplementation. Weight of dried tibia was significantly high (P<0.05) in T4 followed by T2, T3 and Tl at sixth week. Per cent tibial ash at sixth and eighth week were also high (P>0.05) in T2, T3 and T4 compared to Tl on SBR which could indicate that citric acid and/or phytase favour P utilization and bone development. No significant difference \ could be noticed in tibial and serum Ca, P, Mg, Zn and Mn except for a significantly high . tibial Mg (P<0.05) in T4 and serum Zn (P<0.05) in T3 at sixth week. Serum alkaline phosphatase was significantly reduced (P . ., by T3, T2 and Tl. Birds of citric acid, phytase and combination group though maintained on low P diet registered no significant difference in per cent dressed yield, ready-to-cook yield, gib let yield, abdominal fat yield and livability, when compared to Tl fed on SBR. Regarding the cost of production as feed cost/kg gain, phytase group (T3) recorded the lowest due to high cost citric acid. Overall evaluation of the results of the present study revealed that inclusion of either 3.0 per cent citric acid or phytase (700U/kg feed) or its combination (1.5 per cent .citric acid + 350 U phytase/kg feed) in low available P diet (0.3 per cent) resulted in better nutrient utilization and growth performance in chicks than chicks maintained on SBR with 0,5 per cent available P; tending to suggest that available P level in the feed could be reduced by 40 per cent without affecting the performance and health of broiler chicks by dietary inclusion of citric acid and/or phytase.
