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ThesisItem Open Access Cytogenetic studies on intervarietal hybrids of sesamum (Sesamum indicum L.)(Department of Agricultural Botany, College of Agriculture Vellayani, Trivandrum., 1984) Chandramony, D; KAU; Krishnan Nair, NThesisItem Open Access Enhancement of propagation efficiency in exotic varities of heliconia(Department of Pomology and Floriculture, College of Agriculture, Vellayani, 2008) Reshmi, C R; KAU; Sheela, V LHeliconias are among the most popular garden plants, both for the ease with which they can be grown and the sheer magnificence of the blooms. Studies were conducted to standardize rapid propagation techniques under in vitro and in vivo conditions. Three heliconia varieties belonging to three distinct groups viz., St. Vincent Red (small erect type), Golden Torch Adrian (hybrid) and Sexy Pink (large pendent type) were selected for the study. For in vitro culture establishment, shoot tip explants were effective. The treatment of shoot tips with 0.10 per cent mercuric chloride for ten minutes followed by dipping in 0.05 per cent mercuric chloride for five minutes (after trimming) resulted in better surface sterilization. Longitudinal cutting of the in vitro established shoot tip with apical dome into two halves and culturing them separately produced the highest number of axillary buds. Addition of 0.05 per cent activated charcoal to the establishment media reduced the media browning and hastened shoot initiation. For culture establishment of all the three varieties, MS medium supplemented with BA 5.00 mg l-1 was found to be the best. Murashige and Skoog medium supplemented with BA 2.00 mg l-1 resulted in the highest shoot proliferation in the variety St. Vincent Red. In Golden Torch Adrian, BA 2.00 mg l-1 + NAA 0.20 mg l-1 gave better results. In the variety Sexy Pink, Kinetin 5.00 mg l-1 + NAA 0.20 mg l-1 was the best. For further multiplication in Sexy Pink, BA 1.00 mg l-1 was sufficient. Solid culture medium was better for shoot proliferation in the variety Sexy Pink. Higher sucrose concentration (40.00 g l-1) increased the multiplication rate, but reduced the length of shoots as well as the number of leaves. Addition of activated charcoal to the media as well as exposure of cultures to light had negative effect on shoot proliferation. Full MS medium was found to be the best for in vitro rooting of the variety Sexy Pink. Addition of NAA 0.50 mg l-1 to the MS medium gave better results for in vitro rooting in Golden Torch Adrian and Sexy Pink. Sucrose @ 30.00 g l-1 was sufficient for in vitro rooting in the variety Sexy Pink. Activated charcoal delayed root initiation and reduced the number of roots in the variety Sexy Pink. Sand recorded 90.00 per cent survival in all the three varieties after two months of planting out. At varietal level, significant difference was evident in the total number of suckers. In the first experiment, the variety St. Vincent Red (3.82) was significantly superior in terms of total number of suckers. However, in the second experiment, St. Vincent Red (4.06) was on par with Golden Torch Adrian (4.10). The variety Sexy Pink produced comparatively taller suckers in both the trials. Varietal variation in the number of leaves was observed only in the earlier stages. During the preliminary field experiment, the variety Sexy Pink excelled in leaf area at almost all stages of observation. Among the three varieties, highest collar girth was recorded by suckers of Sexy Pink variety during the first two stages of observation. However, towards the later stages, it was statistically on par with St. Vincent Red. Foliar spray was superior to rhizome dip in terms of total number of suckers, height of suckers and the number of leaves. In the case of leaf area, both the treatments were more or less on par. Application of growth regulators had pronounced effect on sucker production at all the stages during the first experiment when BA 750 mg l-1 produced the highest number (4.19) of total suckers. In the second experiment, variation was evident only in the total number of suckers. Here, BA 850 mg l-1 produced the highest number (4.33) of suckers and it was on par with BA 700 mg l-1 (4.00) and GA3 650 mg l-1 (3.79). Irrespective of the stage of plant growth, gibberellic acid produced taller suckers and BA 500 mg l-1 resulted in the shortest suckers. BA treatments recorded comparatively higher number of leaves. Growth regulator application had remarkably influenced the leaf area of suckers also. Application of BA 1000 mg l-1 (3.53 cm) and GA3 800 mg l-1 (3.33 cm) recorded higher collar girth in suckers. VG interaction exerted significant variation in the number of suckers. At varietal level, BA 750 mg l-1 produced the highest number of suckers in St. Vincent Red (4.75), GA3 500 mg l-1 in Golden Torch Adrian (4.63) and GA3 750 mg l-1 in Sexy Pink (4.00). Among VG treatment combinations in the second experiment, the highest number of suckers (4.75) in the variety St. Vincent Red was produced by BA 700 mg l-1. The varieties Golden Torch Adrian (4.88) and Sexy Pink (3.75) recorded the highest with BA 850 mg l-1. Gibberellic acid produced taller suckers. In the second experiment, VG interaction had no significant effect on the height of suckers. Regarding the number of leaves, BA resulted in comparatively higher number of leaves in both the experiments. In all the three varieties, BA 850 mg l-1 produced the highest number of leaves. Collar girth was found to increase with increase in the concentrations of BA and GA3 in all the three varieties. The economics of foliar application of growth regulators revealed that BA 850 mg l-1 significantly enhanced the profit in the varieties Golden Torch Adrian and Sexy Pink. Although negligible, BA 700 mg l-1 recorded slight positive response in the variety St. Vincent Red with respect to profit.ThesisItem Open Access In vitro propagation and rapid analysis of selected varieties of papaya (carica papaya L)(Department of Pomology and Floriculture, College of Agriculture, Vellayani, 2007) Bindhu, B; KAU; Jayachandran Nair, C SA study was carried out at the Department of Pomology and Floriculture, College of Agriculture, Vellayani during 2003-2006 for refining existing tissue culture protocol with respect to selected varieties and hybrids of papaya. Molecular characterization of papaya hybrids was also carried out using Random Amplified Polymorphic DNA (RAPD) technique. Papaya varieties and hybrids selected for the study were Pusa Nanha, CO-5, Washington, Pusa Nanha x Coorg Honeydew, Pusa Dwarf x Coorg Honeydew, Solo x Coorg Honeydew and Pusa Nanha x Solo. Apical buds and lateral buds from seedlings and mature plants were used as explant for in vitro propagation. The present study revealed that full strength MS medium supplemented with sucrose 30.00 g l-1 and agar 6.00 g l-1 under light condition produced highest shoot number and longest shoot in papaya varieties and hybrids. Application of BA 0.50 mg l-1 along with NAA 0.10 mgl-1 was found to be better for initial culture establishment and proliferation of papaya varieties and hybrids. Application of amino acid, arginine 50.00 mg l-1 resulted in highest shoot proliferation rate, while highest shoot length was obtained from arginine 100.00 mg l-1. Addition of activated charcoal 0.05 per cent and Cobalt chloride 10.00 mg l-1 increased shoot proliferation rate and shoot length in papaya varieties and hybrids. In vitro rooting was best in full strength MS medium supplemented with IBA 3.00 mg l-1, sucrose 30.00 g l-1and activated charcoal 0.05 per cent. Earliest planting out of in vitro propagated plants in potting media was carried out in papaya variety Pusa Nanha and late planting out in papaya hybrid Pusa Nanha x Coorg Honeydew. Highest plant height of in vitro propagated plants, at time of planting out in potting media was recorded by papaya variety Washington and lowest by papaya hybrid Pusa Nanha x Coorg Honeydew, while hsighest leaf number was reported from papaya variety CO-5 and lowest from Pusa Nanha. But longest root was noticed in papaya hybrid Pusa Nanha x Coorg Honeydew and shortest root from CO-5. No significant difference was noticed in the number of roots produced at the time of planting out in potting media. In vitro propagated plants of papaya had better establishment in the potting mixture containing sand : soil : cowdung (1 : 1 : 1) + Mycorrhiza (1.00 g plant -1). After field establishment, highest plant height and plant girth was noticed in papaya variety Washington and lowest in papaya hybrid Pusa Dwarf x Coorg Honeydew. While highest leaf number upto six weeks after planting was shown by papaya variety CO-5 and thereafter by papaya variety Washington. Lowest leaf number upto eight weeks after planting was noticed in papaya hybrid Solo x Coorg Honeydew and thereafter by papaya hybrid Pusa Dwarf x Coorg Honeydew. Early flowering was reported in papaya hybrid Pusa Dwarf x Coorg Honeydew and late flowering in papaya variety Washington. Most of the papaya varieties and hybrids selected for the present study exhibited only dioecious nature, but papaya hybrid Solo x Coorg Honeydew showed gynodioecious nature. Highest field establishment of in vitro propagated plants was shown by papaya varieties CO-5 and Washington and lowest by papaya hybrid Solo x Coorg Honeydew. Genomic DNA was successfully isolated from four papaya hybrids using CTAB method. All the ten primers (six primers from OPA and four primers from OPB series) yielded amplification products with isolated DNA. A total of 39 RAPDs were obtained from ten primers of which 36 bands were polymorphic. Primers OPA-03, OPA-04, OPA-12, OPB-04 and OPB-17 gave 40 scorable bands, with an average of 8.0 bands per primer. The largest cluster in dendrogram was formed by three hybrids - Pusa Nanha x Coorg Honeydew, Pusa Dwarf x Coorg Honeydew and Pusa Nanha x Solo. The second cluster contained only one hybrid - Solo x Coorg Honeydew. The minimum similarity coefficient detected in the present study was 0.389, suggesting a genetic differentiation among the papaya hybrids.ThesisItem Open Access Induced mutations in banana var. Nendran(Department of Agricultural Botany, College of Agriculture, Vellayani, 1990) Radha Devi, D S; KAU; Krishnan Nair, NThe present investigations was carried out in the Department of Agricultural Botany, College of Agriculture, Vellayani during 1985-88 and in the plant tissue culture Laboratory attached to the Department of Plantation Crops, College of Horticulture, Vellanikkara, Thrissur during 1986-88. The project was taken up to standardise the techniques for induced mutagenesis in-vitro and in-vitro in banana (Musa paradiciaca L.) var. nendran and aslo to analyse the direct effect of 60 Co gamma rays on growth and bunch characters in the vM1 and vM3 generations. One, two and three months old suckers of various sizes (after removal of 25 to 75 per cent of the pseudostem) were exposed to 1.0, 1.5, 2.0, 2.5 and 3.0 kR gamma rays. For in-vitro mutagenesis, isolated shoot tips were exposed to 0.50, 0.75, 1.00, 1.25 and 1.50 kR gamma rays. Ex-vitro analysis of five plants per treatment per exposure was done in the vM1 generation for various growth, bunch and fruit characters.ThesisItem Open Access Studies on heterosis and combining abilities with respect to important economic traits in Capsicum annuum, L.(Division of Agricultural Botany, College of Agriculture, Vellayani, 1981) Manikantan Nair, P; KAU; Mary, K GeorgeSixty three varieties of Capsicum annuum representing different agroclimatic regions of the country constituted the base material of the study. Based on yield potential , adaptability and tolerance to leaf curl complex disease, thirty varieties were selected for subsequent studies. Comprehensive genetic studies including estimation of genetic parameters, cause effect relationship by path coefficient analysis, genetic divergence by Mahalanoble D2 statistic and a nine parent diallel analysis to causes the combining ability, to unravel the pattern of inheritance and also to examine the level of heterosis manifested with respect to eighteen economic characters including four nutritiveand quality attributes namely Vitamin A, Vitamin C, Capsaicin and Oleoresin content were conducted during the year 1976-79. Analysis of plot means exhibited highly significant differences among varieties except for Capsaicin content. The high degres of variability in economic attributes offers scope for recombining desirable genes from different varieties.ThesisItem Open Access Biometric analysis of yield and certain yield attributes in the para rubber tree: Hevea Brasiliensis Muell. Arg.(Department of Agricultural Botany, College of Agriculture, Vellayani, 1984) Markose, V C; KAU; Mary, K GeorgeThe para rubber tree, Hevea brasiliensis, is the commercial source of natural rubber. Introduced into the Far East in 1876, from Brazil, commercial cultivation started in India during 1902. The tree has a gestation period of six to seven years. A large number of clones have been evolved through ortet selection and through hybridisation, which show variability for different characters. The present study was undertaken to estimate (1) productivity (2) yield pattern (3) variability (4) correlations (5) effect of yield attributes (6) genetic diversity of twenty clones, and (7) early growth vigour of open pollinated seedlings of selected clones.ThesisItem Open Access Genetic analysis in red gram (Cajanus cajan L.)(Department of Agricultural Botany, College of Horticulture, Vellanikkara, 1990) Achamma Oommen; KAU; Narayanan Namboodiri, K MThe research project "Genetic analysis in red gram" was carried out in the College of Horticulture, Kerala Agricultural University, Vellanikkara, Trichur during the period 1985-90. A 5 x 5 diallel cross, excluding reciprocals conducted in red gram revealed the predominance of additive gene effect for all the characters except days to first flowering. The combining ability analysis showed PLA-550 as the best general combiner; and the F 1 of the cross UPAS-120 x I.C. 15708 to have good specific combining ability. Negative heterosis over both the parents was exhibited by UPAS-120 x I.C. 15708 and PLA-550 x PLA-600 for days to first flowering and days to maturity which showed that early maturing segregants could be obtained from these crosses. The same crosses showed relative heterosis for yield also. The analysis of generation means by using six parameter model revealed the importance of both additive and dominance gene effects in the expression of most of the characters. Additive x additive and dominance x dominance effects showed influence in the expression of the characters. Among the components of variances dominance variance was higher for most of the characters. Heritability in narrow sense was found to be low. Number of clusters per plant and number of pods per plant were highly correlated with yield in parents, F 1 's and F2's. As the yield and yield components in the present study were under all the three types of gene action viz., additive, dominance and epistasis, reciprocal recurrent selection procedure seems to be the best suited method of breeding for improvement of this crop.ThesisItem Open Access Seed germination and tissue culture studies in orchids(Division of Horticulture, University of Agricultural Sciences, Bangalore, 1982) Ramachandran Nair, S; KAU; Foja SinghThesisItem Open Access Genetic studies in brlnjal with relation to bacterial wilt resistance(Department of Agricultural Botany, College of Agriculture, Vellayani, 1983) Gopimony, R; KAU; Krishnan, Nair N
