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ThesisItem Open Access Quality evaluation of vegetables cultivated through aquaponics(Department of Community Science, Vellayani, 2020) Bakam, Himabindhu; KAU; Suma, DivakarThe project entitled “Quality evaluation of vegetables cultivated through aquaponics” was carried out at the Department of Community Science, during 2018-2020. The objective of the study was to compare the quality characteristics of selected green leafy vegetables cultivated through aquaponics (T1) and conventional methods (T2). The capacity of the grow bed of the aquaponics unit selected was 400 L and that of the fish tank was 500 L.tuberless colocasia, water spinach and malabar spinach were the selected green leafy vegetables.Hundred tilapia fishes were grown with the plants. After the plants attained maturity, the physical characteristics, sensory qualities, nutrient composition, nutraceutical composition and shelf life of the two treatments of these leafy vegetables were studied. Except for water spinach, yield of malabar spinach and tuberless colocasia were comparable in both the treatments. Dry matter production of T1tuberless colocasia(119.5g/plant) was higher than T2 (45.25g/plant). Higher values for sensory qualities in both fresh and cooked samples of T1were obtained with respect to colour, appearance, texture, taste and flavor. In the case of water spinach, the higher scores obtained for appearance and colour of fresh leaves were significantly different (z value-3.023 and 2.305 respectively). Nutrient analysis revealed significant differences in the moisture content and fibre content of malabar spinach among the treatments. Statistical analysis revealed significant difference among the treatments cultivated in aquaponics and conventional systems for tuberless colocasia and malabar spinachwith respect to total minerals and acidity content. Nutrient analysis revealed significantly highervalues for soluble sugars, vitamin C, beta carotene, calcium and Iron for T1. In nutraceutical profile analysis, except for oxalate content, there was significant differences among the treatments. 138 Shelf life studies revealed that tuberless colocasia (T1) remained fresh for 7 days when packed in newspaper, while T2tuberless colocasia, kept only for 6 days in similar packing. water spinach had the least shelf life in ambient conditions -T1 had 4 days and T2 had 3 days, when packed in news paper. Physiological loss of water was not found to be significantly different in any of the treatments of these vegetables. Shelf life of leaves were not seen to show differences among the treatments. Packing in PP covers were generally seen to be ideal for all the three leaf varieties. Overall analysis of the results revealed that, physical characteristics, sensory qualities, nutrient composition, nutraceutical composition and shelf life parameters of T1were on par with T2, or even higher in most cases, indicating the scope of raising these vegetables successfully in households for Food and Nutrient security.ThesisItem Open Access Germination and plant growth responses in Ocimum spp. to seed pretreatments(Department of Plantation Crops and Spices, College of Agriculture , Vellayani, 2020) Akhil Raj, B C; KAU; Deepa Nair, SGermination and plant growth responses in Ocimum spp. to seed pretreatments The present investigation entitled “Germination and plant growth responses in Ocimum spp. to seed pretreatments” was conducted in the Department of Plantation Crops and Spices, College of Agriculture, Vellayani during 2018-2020 with the objective to standardize pretreatment of seeds for enhanced germination and plant growth in Ocimum tenuiflorum L. and Ocimum basilicum L. The seeds of Ocimum tenuiflorum and Ocimum basilicum used for the study were sourced from Indian Institute of Horticultural Research, Bengaluru. The study was carried out in two phases: Phase 1- Pretreatment of seeds for enhanced germination, Phase 2- Evaluation of transplanted seedlings derived from pretreated seeds for enhanced plant growth. The seeds of both the species of Ocimum were subjected to various pretreatments viz., physical pretreatments, hormonal priming, biostimulant priming and biopriming (using microbes) prior to sowing. The seeds subjected to germination without any pretreatments were taken as the control. In the first phase of the study, O. tenuiflorum seeds when exposed to physical treatments, pretreatment using concentrated sulphuric acid for 1 min recorded maximum germination (85.33 per cent). The hot water treatment (65oC for 10 min) recorded the highest seedling length (27.83 cm) and seedling vigour index (19.67) with a germination of 70.66 per cent. Among the hormonal treatments, seeds primed with GA3 @1500 μM recorded the highest germination (96 per cent), seedling length (29.63 cm) and seedling vigour index (28.42). In biostimulant seed priming, the untreated control recorded the highest germination (62.66 per cent) but the highest seedling length (31.29 cm) and seedling vigour index (11.46) were observed with salicylic acid (SA) @ 1500 μM . The . The . The . The seeds when subject to biopriming with microbes, seeds primed with Bacillus pumilus recorded the best germination (72.66 per cent) and seedling vigour index (15.83). The highest seedling length (22.05 cm) was observed with Pseudomonas fluorescens, which was on par with that of B. amyloliquefaciens. Among all the pretreatments tried, GA3 @ 1500 μM was observed to give maximum germination (96 per cent) and seedling vigour index of 28.42, while SA @ 1500 μM recorded the highest seedling length (31.29 cm) in O. tenuiflorum. When transplanted, O. tenuiflorum plants derived from hot water treated seeds recorded the highest number of branches (45.33), fresh (130.10 g) and dry (31.22 g) shoot biomass at harvest (90 DAS), among the physical treatments. Plants derived from GA3 @ 1500 μM treated seeds recorded the highest shoot biomass in terms of fresh (193.50 g) and dry (46.13 g) weight. The plants from SA @ 1500 μM μM primed seeds- gave the highest shoot biomass with fresh weight of 217.50 g and dry weight of 54.10 g, among various biostimulant priming treatments. Among the biopriming treatments, plants generated from the seeds treated with B. amyloliquifaciens, recorded the highest fresh (151.33 g) and dry (38.84 g) shoot biomass which was on par with that treated with P. fluorescens. Among all the pretreatments, plants derived from salicylic acid @ 1500 μM treated seeds recorded the highest shoot biomass. When the O. basilicum seeds were exposed to physical treatments, water soaking treatment recorded the maximum seedling vigour index (16.13), while seedling length did not show any significant variation among the treatments. Among the hormonal priming, seeds exposed to BA @ 300 μM recorded the highest germination (80.67 per cent) and seedling vigour index (29.33). The maximum seedling length (39.03 cm) was observed in GA3@ 3000 μM . The seeds treated with SA @ 1500 μM recorded the highest germination (79.33 per cent) and seedling vigour index (27.92), among the biostimulant priming. Maximum seedling length (35.66 cm) was observed in SA @ 3000 μM and was and was and was and was on par with on par with on par with on par with SA @1500 μM . Among the biopriming treatments, seeds primed with B. velezensis recorded higher germination (82 per cent), seedling length (37.50 cm) and seedling vigour index (30.72), which were on par with P. fluorescens and B. amyloliquifaciens. In O. basilicum, among all the pretreatments tried, biopriming with B. velezensis, B. amyloliquefaciens and P. fluorescens and SA @ 1500 μM recorded higher on par values with respect to germination per cent and seedling vigour index, while GA3 @3000 μM which recorded the highest seedling length. When transplanted, plants of O.basilicum derived from the seeds exposed to physical treatments did not show any significant variation with respect to shoot biomass. In hormonal priming, the maximum fresh (146.00 g) and dry (17.39 g) shoot biomass were observed in plants generated from seeds primed with GA3 @ 3000 μM . On evaluation of plants derived from biostimulant priming, maximum fresh (134.17 g) and dry (15.95 g) shoot biomass were observed in SA@ 3000 μM, which was on par with SA @ 1500 μM μM, which was on par with SA @ 1500 μM μM, which was on par with SA @ 1500 μM μM, which was on par with SA @ 1500 μM μM, which was on par with SA @ 1500 μM μM, which was on par with SA @ 1500 μM μM, which was on par with SA @ 1500 μM μM, which was on par with SA @ 1500 μM μM, which was on par with SA @ 1500 μM μM, which was on par with SA @ 1500 μM μM, which was on par with SA @ 1500 μM μM, which was on par with SA @ 1500 μM μM, which was on par with SA @ 1500 μM μM, which was on par with SA @ 1500 μM μM, which was on par with SA @ 1500 μM μM, which was on par with SA @ 1500 μM μM, which was on par with SA @ 1500 μM . Among the biopriming treatments, plants derived from seeds primed B. velezensis recorded the highest fresh (153.03 g) and dry weight (17.73 g) shoot biomass, which were on par with P. fluorescens and B. amyloliquefaciens. Among all the seed pretreatments tried, plants generated from the seeds primed individually with B. velezensis, B. amyloliquefaciens and P. fluorescens and SA @ 1500 μM recorded higher on par values with respect to shoot biomass. In the study, among the various seed pretreatments in O. tenuiflorum, GA3 @ 1500 μM recorded enhanced germination and plant growth, in terms of germination per cent, seedling length, seedling vigour index, plant height, number branches and shoot biomass. In case of O. basilicum, biopriming using B. velezensis, B. amyloliquefaciens and P. fluorescens recorded enhanced germination and plant growth, in terms of higher germination per cent, seedling length, seedling vigour index, leaf, stem and shoot biomass.ThesisItem Open Access Evaluation of elite clove (Syzigium aromaticum (L.) Merr. and Perry) accessions and standardization of pollination techniques(Department of Plantation Crops and Spices, College of Agriculture , Vellayani, 2020) Reddappa, J B; KAU; Sreekala, G SThe present study entitled “Evaluation of elite clove (Syzigium aromaticum (L) Merr. & Perry) accessions and standardization of pollination techniques” was taken up with the specific objective to evaluate elite clove accessions and to standardize pollination techniques for hybridization. The twelve elite accessions identified from estates of Trivandrum and Kollam districts of Kerala designated as BRC-1, BRC-2, BRC-3, BRC-4, MRC-5, MRC-6, MRC-7, MRC-8, AMC-10, AMC-11, AMC-12 and AMC-13 were evaluated during the period of 2018-2020. Quantitative characterization was done with 25 quantitative characters including tree, leaf, bud, flower, yield, fruit and seed characters. The girth of the tree at 30 cm height was maximum in BRC-3 (165 cm). Highest number of inflorescence/m2 was recorded in AMC-12 (156.25). The number of flower buds/inflorescence was maximum in MRC-6 (17.25) while single bud fresh and dry weight was highest in BRC-3 with 398.72 mg and 127.26 mg respectively. The mature bud length among the clove accessions was the maximum in BRC-1 (19.34 mm) whereas the mature bud diameter was maximum in BRC-3 (6.45 mm). The dry bud yield per tree was the highest in MRC-7 (8.13 kg) followed by BRC-1 (7.48 kg). Quantitative characters summarized based on the descriptive statistics revealed wider range of variability in single bud weight fresh, number of inflorescence/m2, single bud weight dry and girth of the tree. Multi Dimensional Scaling (MDS), a multivariate analysis done on 25 variables with 12 observations revealed 10 dimensions and the contribution of MDS Dimension-1 and Dimension-2 were 75.76 and 17.21 percent with cumulative variance of 92.97 percent respectively. Based on the scree plot of MDS, first 2 dimensions were selected for generating the biplot. Based on MDS configuration eight clusters of selected clove accessions were identified. Dendrogram representing the relationship among 12 elite accessions based on Euclidean distance also identified same eight clusters of clove accessions that identified in MDS configuration. The linkage analysed based on the biplot of two dimensions of multidimensional scaling of biplot coordinates revealed a strong positive association between most of the characters measured except, girth at 30 cm height, number of inflorescence per m2, single bud weight fresh and single bud weight dry. Minimum Data Set for identifying a promising clove accession generated revealed accessions BRC-1, MRC-5 and MRC-6 had better ideotype and can be suggested as elite or superior accessions. The experiment on floral phenology and biology was carried out in clove plants at Braemore estate, Trivandrum district. The flower opening time of the clove flowers observed were between 2:30-6:30 pm. However the percentage of flower opened was maximum between 3.30- 5.30 pm. The number of stamens per flower varied from 167 to 343. The rupturing of anthers started 36 hours before anthesis and maximum anther dehiscence occurred between 36 h before anthesis till anthesis. The pollen grains were monad, radially symmetrical, triangular, trizonosyncolporate and were having exine ornamentation. The polar diameter of pollen grain ranged from 17.06-18.22 µm and the equatorial diameter of pollen grain ranged from 19.40-20.70 µm. The stigma receptivity by hydrogen peroxide test revealed stigma receptivity was there upto six days from anthesis and maximum stigma receptivity was observed on fifth day of anthesis with highest number of bubbles (31.40 per cent). The emasculation should start from 48 hour before flower bud opens which was revealed by the starting of separation of the petals since anthers started rupturing from 36 hour before anthesis. Pollen viability by iodine potassium iodide method revealed that pollen viability was maximum at 12 hour before anthesis (94.02 per cent). Pollen fertility was also maximum on the pollen collected 12 hour before anthesis (82.83 per cent). Thus the pollen collection should be undertaken 12 hour before anthesis. Pollen collected and dried at 50°C and stored upto two months of storage in refrigerator had maximum pollen viability whereas pollen fertility was maximum in pollen dried at 50°C and stored in desiccator. The hybridization was carried out on selected five clove plants as male and female parents. The percentage of fruit set ranged from 15 per cent to 42.5 per cent and average fruit set from all the plant combinations was 28.87 per cent. The study on “Evaluation of elite clove (Syzigium aromaticum (L) Merr. & Perry) accessions and standardization of pollination techniques” revealed that clove accessions such as BRC-1, MRC-5 and MRC-6 had superior ideotype and superior in effective yield. The floral phenology of clove was studied and pollen collection, storage and artificial hand pollination in clove was standardized.ThesisItem Open Access Development of F1 hybrids of indeterminate tomato (Solanum lycopersicum L.) for protected cultivation(Department of Olericulture, College of Agriculture, Vellayani, 2015) Lekshmi, S L; KAU; Celine, V AThe present investigation entitled “Development of F1 hybrids of indeterminate tomato (Solanum lycopersicum L.) for protected cultivation” was conducted at the Department of Olericulture, College of Agriculture, Vellayani, from 2013 to 2015 with the objectives of identifying superior varieties and developing F1 hybrids of indeterminate tomato suited for protected cultivation. The study consisted of two experiments conducted in the naturally ventilated polyhouse of size 50 m x 20 m located at the Instructional Farm, Vellayani. In the first part of the first experiment, 40 tomato genotypes were evaluated for two consecutive years in an RBD with three replications. As the second part, 12 commercial hybrids were evaluated. The second experiment consisted of a 9 x 9 half diallel analysis laid out in an RBD with three replications. Analysis of variance showed significant differences between the genotypes for all the characters for two crops. Pooled analysis revealed that, LE 1 recorded the highest yield (2443.43 g) and fruit weight (108.13 g) followed by LE 7. LE 53 had maximum number of fruits per plant (65.00). In the present study, genotypes had wide variation for quality parameters. Fruits of LE 14 recorded highest TSS with a mean of 5.74 ºBrix. LE 7 had maximum ascorbic acid (30.13 mg/100g) and lycopene content (13.09 mg/100g). Beta carotene value was maximum in LE 16 (184.15 mg/100g). There was minimum incidence of pests, diseases and physiological disorders under protected conditions. Among the 12 hybrids evaluated, INDAM 9802 was the highest yielder (1444.40 g) followed by F1 T 30 (1412.22 g). F1 T 30 recorded maximum fruits per plant (35.66) which was on par with F1 Queen (35.55). Genetic parameters like phenotypic and genotypic coefficients of variation, heritability and genetic advance were studied to assess the genetic variability among the genotypes. High heritability coupled with high genetic advance were observed for characters like truss per plant, fruits per truss, fruit weight, fruits per plant, yield per plant and yield per plot. Path analysis revealed highest positive direct effect for fruit weight (0.3956), truss per plant (0.3558) and fruits per plant (0.3381). Based on D2 analysis the 40 genotypes were grouped into eight clusters. Cluster I was the largest with twenty four genotypes followed by cluster II with ten genotypes. Diallel analysis was carried out using nine parents selected based on genetic divergence and per se performance. The parents were crossed in a diallel fashion excluding reciprocals to obtain 36 F1 hybrids. The study revealed that P5 x P9 had the highest yield (3114.03 g) which was on par with P6 x P8 (3074.37 g) and P1 x P5 (3077.58 g). P1 x P5 had the maximum fruits per plant (103.93). The magnitude of relative heterosis, heterobeltiosis and standard heterosis varied considerably. For yield, relative heterosis ranged from -32.40 to 92.72, heterobeltiosis from -47.14 to 89.54 and standard heterosis from 2.91 to 160.95. The σ2gca and σ2sca ratio indicated that non-additive gene action was predominant for all traits. Among the nine parents, P9 (LE 1), P5 (LE 20) P1 (LE 2), and P2 (LE 7) were superior for yield and yield attributes. The estimates of sca effects indicated that P5 x P9 (LE 20 x LE 1), P6 x P8 (LE 39 x LE 38) and P1 x P5 (LE 2 x LE 20) were the most promising hybrids for protected cultivation. The present study revealed that the genotypes LE 1 and LE 7 and the hybrids INDAM 9802 and F1 T 30 were superior for yield and yield attributes under protection. Based on the mean performance, standard heterosis and sca effects the three potential crosses viz., P5 x P9, P6 x P8 and P1 x P5 could be adjudged as suitable indeterminate tomato hybrids for protected cultivation.ThesisItem Open Access Antioxidant and anticarcinogenic potential of jackfruit based ready-to-cook (RTC) curry mixes(Department of Community Science, College of Agriculture,Vellayani, 2019) Gayathri Mohan; KAU; Suma DivakarThe research work entitled, “Antioxidant and anti-carcinogenic potential of jackfruit based Ready-To-Cook (RTC) curry mixes” was conducted at College of Agriculture, Vellayani during 2017-2019, with the objective to ascertain the antioxidant and anti-carcinogenic properties of the jackfruit based curry mixes (‘Avial’ mix, ‘Koottu’ mix and ‘Ularth’ mix) which were developed earlier at the Department of Community Science, using raw jackfruit (Koozha type). Methanol, ethanol, petroleum ether and acetone were used to extract the bioactive compounds of the powdered mixes for phytochemical screening, antioxidant analysis and verification of anti-carcinogenic property. The results of the phytochemical analysis indicated the presence of tannins, flavonoids, saponins, steroids, phenolic compounds, cardiac glycosides and anthraquinones in the three mixes; whereas the analyses revealed the absence of alkaloids and phlotobatinins. Quantitative analysis of antioxidants revealed that the beta carotene content of the RTC mixes was in the range of 0.07-1.16 mg/100g. Beta carotene content was found to be more in ‘Avial’ mix (1.16mg/100g). Ascorbic acid content was also found to be higher for ‘Avial’ mix (38.45 mg) and the lowest content was found for ‘Ularth’ mix (15.34 mg). The saponin content was found to be highest in ‘Avial’ mix (6.65 per cent) and lowest in ‘Ularth’ mix (5.00 per cent). The tannin content of ‘Ularth’ mix (11.57 mg) was observed to be on par with ‘Avial’ mix (11.52 mg). Total phenol content was found to be more in ‘Koottu’ mix (21.53 mg/100g) and the total flavonoid content of RTC mixes was seen to be in the range between 0.86-3.25 mg/100g. There was significant difference in the lectin content of ‘Avial’ mix (0.35%), ‘Koottu’ mix (0.56%) and ‘Ularth’ mix (0.75%). Alkaloids and selenium were found to be absent in the RTC mixes. In case of mineral analyses, Copper content was found to be higher in ‘Ularth’ mix (2.57 µg); zinc content was in the range of 4.65-6.55 µg and was found to be higher for ‘Koottu’ mix (6.55 µg) and lower for ‘Ularth’ mix (4.65 µg). Manganese content was found to be higher in ‘Koottu’ mix (5.30 µg) while, it was observed to be on par in ‘Avial’ mix (2.55 µg) and ‘Ularth’ mix (2.51 µg). The highest iron content was found in ‘Ularth’ mix (0.92 µg). Antioxidant activity in the present study revealed that ‘Avial’ mix had the highest DPPH activity with an IC50 value of 33.81 µg/ml. Total antioxidant activity was found to be more for ‘Avial’ mix (41.44 per µg/ml), followed by ‘Koottu’ mix (42.41 µg/ml) and ‘Ularth’ mix (43.45 µg/ml). ‘Avial’ mix showed more ferric reducing capacity while ‘Ularth’ mix had the least capacity in this regard. ABTS radical scavenging activities of RTC mixes ranged between 34.84-46.69 µg/ml. ABTS radical scavenging activity was observed to be higher for ‘Avial’ mix (34.84 µg/ml) and lower values were noted for ‘Ularth’ mix (40.52 µg/ml). Hydroxyl radical scavenging activity of RTC mixes was found to range between 50.55-52.55 µg/ml. ‘Avial’ mix showed higher superoxide radical scavenging activity with an IC50 value of 48.54 µg/ml and the lowest superoxide radical scavenging activity was observed in ‘Ularth’ mix (60.73 µg/ml). However, the highest nitric oxide scavenging activity was observed for ‘Ularth’ mix (14.11 µg/ml) followed by ‘Koottu’ mix (30.92 µg/ml) and ‘Avial’ mix (36.58 µg/ml). Among the three jackfruit based Ready-To-Cook (RTC) curry mixes ‘Avial’ mix was found to have higher antioxidant property and was therefore taken up for studying the anti-cancer property. Cytotoxicity of ‘Avial’ mix was estimated by MTT assay on Human breast adenocarcinoma cells (MCF-7) and 17 per cent of loss in cell viability was observed. Findings of the present study revealed that the jackfruit based RTC mixes comprised of various health promoting components such as beta carotene, ascorbic acid, saponins, tannins, total phenols, total flavonoids and lectins they also had potent antioxidant activities. Thus the curry mixes, in particular the ‘Avial’ mix can be promoted as functional food which will help to enhance its commercialization.ThesisItem Open Access Standardization of hydroponics fodder production technology(Department of Agronomy, College of Agriculture, Vellayani, 2019) Sruthi Liz Thomas; KAU; Usha C ThomasThe study entitled “Standardization of hydroponics fodder production technology” was undertaken during 2017-2019, at College of Agriculture, Vellayani, Thiruvananthapuram, Kerala, with the objectives to identify suitable fodder crops for hydroponics system and to standardize nutrient solution, seed rate and period for harvest. Research work comprised three experiments which was carried out in a low cost hydroponics fodder production system at the Integrated Farming System Research Station, Karamana, Thiruvananthapuram. The crops were grown in a hydroponics machine made of PVC pipes with 2m x 1.3m x 1.8m length, breadth and height with four shelves and automatic sprinkler irrigation system. The first experiment was aimed to evaluate the suitability of fodder crops for hydroponics. The experiment was laid out in completely randomised design and the crops included in the trial were rice (c1), barley (c2), maize (c3), wheat (c4), sorghum (c5), bajra (c6), ragi (c7), cowpea (c8), horse gram (c9) and greengram (c10). The seeds were soaked in 0.1 per cent sodium hypochlorite solution for 12 hours, tied in gunny bag and kept for germination for 24 hours. The seeds were then transferred to trays following a seed rate of 200 g ft-2 (0.22 g cm-2). Among the crops, maize recorded the highest net income and B:C ratio. Considering yield and quality, greengram recorded significantly superior GFY (10.17 kg kg-1seed), protein content (20.97 %), the lowest values for fibre and ash, and a B:C ratio more than one. In sorghum and pearl millet, seed to green fodder yield multiplication was very poor (<3 kg). Finger millet had undesirable characters for hydroponics green fodder like lower values for shoot length, shoot weight, protein content, EE and higher values for fibre and ash. Hence, maize and greengram were identified and selected for the next experiment. The second experiment was done to standardize seed rate and period of harvest of the selected crops. Three different seed rates viz, s1 : 150 g ft-2 (1.61 kg m-2), s2: 175 gft-2 (1.88 kg m-2), s3 : 200 g ft-2 (2.15 kg cm-2) and four different period for harvest viz, t1 : 7 days, t2 : 9 days, t3 : 11 days and t4 : 13 days were adopted. But, in the case of green gram, it was observed that the crop did not survive beyond nine days in hydroponic fodder production system and hence only two periods of harvest viz, t1 and t2 were taken for green gram. Both crops were separately analysed in completely randomised design with two factors. It was observed that maize sown at 200 g ft-2 (s3) and harvested on 11th day (t3) recorded significantly higher values for GFY (6.03 kg kg-1seed) and B:C ratio. While, greengram sown at 200 g ft-2 (s3) and harvested on 7th day (t1) recorded significantly higher values for GFY (10.18 kg kg-1seed) and B:C ratio. Hence, these best combinations of seed rate and period of harvest for both the crops were selected for the next experiment. The third experiment was done to standardize nutrient solution for hydroponics fodder production. The nutrient solutions tested were n1: hoagland solution (0.25%), n2: hoagland solution (0.5%), n3: 19:19:19 (0.5%), n4 : DAP (0.5%) + KCl (0.5%), n5 : starter solution (1:2:1), n6 : vermiwash (10 times dilution), n7 : groundnut cake (supernatant solution) and n8 : water (control), which was sprayed on alternate days from 3rd day. Both crops were separately analysed in completely randomised design. In green gram, spray of hoagland solution (0.25%) (n1) resulted in higher shoot weight, root weight, root length, shoot length and GFY, but the B:C ratio was less than one. Spray of 19:19:19 (0.5%) (n3) recorded on par values with hoagland solution (0.25%) (n1) for shoot weight, root weight, root length, shoot length, GFY and a significantly higher B:C ratio. In maize, spray of hoagland solution (0.25%) (n1) resulted in higher shoot weight, root weight, root length, shoot length and GFY, but a B:C ratio of less than one. The highest B:C ratio was recorded in treatment n8 i.e, water (control). From the results, it can be concluded that both maize and greengram are suited for hydroponics fodder production. Maize grown at a seed rate of 200 g ft-2 with water and harvested on 11th day, and greengram at a seed rate of 200 g ft-2, sprayed with 19:19:19 (0.5%) and harvested on 7th day were found to give the best results considering growth parameters, yield attributes, quality and economics.ThesisItem Open Access Screening of pink pigmented facultative methylotroph isolates for water stress tolerance and yield in paddy(Department of Agricultural Microbiology, College of Agriculture, Vellayani, 2019) Riyas, N K; KAU; Meenakumari, K SThe study entitled “Screening of Pink Pigmented Facultative Methylotroph (PPFM) isolates for water stress tolerance and yield in paddy” was undertaken during 2017-2019, in the Department of Agricultural Microbiology, College of Agriculture, Vellayani, Thiruvananthapuram, with the objective to screen the Pink Pigmented Facultative Methylotroph (PPFM) isolates for water stress tolerance and yield in paddy. The study comprised an in vitro screening experiment and a pot culture experiment with rice variety Harsha. For in vitro screening of PPFM isolates for water stress tolerance, 20 isolates of PPFM from paddy were selected from the previous study of M.Sc. (Ag.) thesis work conducted in the Department of Agricultural Microbiology, Vellayani during 2015-2017 on the basis of carotenoid pigment production, IAA production, proline content, seedling vigour index and yield. These isolates were screened by paper towel method for water stress tolerance under in vitro conditions using mannitol for inducing osmotic stress. There were 21 isolates (20 KAU isolates of PPFM and one TNAU isolate) and four water stress levels (1%, 2%, 3% mannitol and control). The experiment was laid out in completely randomized block design with two replications. Osmotic stress was higher in 3 per cent mannitol treatment. Seeds treated with PPFM 26 recorded the highest germination percentage, shoot length and seedling vigour index. The highest root length and shoot dry weight were observed with the isolate PPFM 15 whereas the highest root dry weight was recorded with PPFM 9. Scoring was done to assess the best five isolates and those with higher ranks were selected for the subsequent experiment. Consequently, PPFM 26, PPFM 15, PPFM 38, PPFM 37 and PPFM 35 which secured ranks from 1 to 5 were selected for the pot culture experiment. The pot culture experiment was undertaken to study the effect of PPFM isolates on growth and yield of paddy under water stress. The experiment was laid out in CRD with 21 treatments and three replications, during summer 2019. The treatments comprised six PPFM isolates (5 KAU isolates of PPFM and one TNAU isolate) and three moisture levels (at field capacity, 75% available water and 50% AW) and three control treatments (0.5% methanol, AMS liquid medium supplemented with 0.5% methanol and absolute control). The treatments were given as seed treatment, seedling root dip and foliar application at 15 and 30 DAT. The study revealed that PPFM isolates had significant effect on biometric parameters, physiological parameters, yield and yield attributes of paddy under water stress. Maximum plant height and leaf area index was recorded with PPFM 38 at 30 DAT and 60 DAT whereas PPFM 37 recorded maximum number of tillers per hill at 60 DAT. Leaf rolling score and leaf drying score were found to be the lowest with PPFM 37 at 30 and 60 DAT. Cell membrane integrity, relative water content, chlorophyll stability index and root dry weight were the highest with PPFM 38 at 30 and 60 DAT. Rooting depth was the highest with PPFM 38 at 30 DAT and PPFM 37 at 60 DAT. Proline content (at all the three moisture levels) and super oxide dismutase (SOD) were significantly higher with PPFM 37 at 30 and 60 DAT. While at 60 DAT, PPFM 37 recorded significantly higher catalase activity at FC and 50% AW, PPFM 38 was found to be superior at 75% AW. Both these isolates were comparable at the different moisture levels. Crop treated with PPFM 37 also recorded the lowest drought susceptibility index. However, peroxidase activity was significantly higher with PPFM 38 at all moisture levels at 30 and 60 DAT. All the PPFM isolates had significant effect on yield attributes and yield of paddy under water stress. Maximum number of panicles per hill, number of grains per panicle (at all moisture levels), grain yield and the lowest relative percentage yield reduction was recorded with PPFM 37. While, PPFM 37 recorded significantly higher number of panicles per hill at FC and 75% AW, PPFM 38 was found to be superior at 50% AW. Both these isolates were comparable at different moisture levels. Though PPFM 37 recorded higher grain yield at all the moisture levels it was on par with PPFM 38. Considering the major drought tolerance parameters such as leaf rolling score, leaf drying score, rooting depth, proline content, SOD, catalase and peroxidase, PPFM 38 was ranked first among the PPFM isolates tested in the pot culture experiment. With respect to the yield attributes and yield of rice under water stress the effect of PPFM 37 and PPFM 38 were observed to be at par. The present study revealed that the isolates PPFM 37 and PPFM 38 (seed treatment 1% PPFM broth culture + seedling dip 2% PPFM broth culture + foliar spray 1% PPFM broth culture at 15 and 30 DAT) were effective in improving the growth, yield and drought tolerance characters of rice.ThesisItem Open Access Evaluation of pongamia oil soap against major pests of okra, Abelmoschus esculentus (L). Moench(Department of Agricultural Entomology, College of Agriculture, Padannakkad, 2019) Anu Thomas; KAU; Sreekumar, K MThe proposed study entitled, Evaluation of pongamia oil soap against major pests of okra, Abelmoschus esculentus (L). Moench was aimed at evaluating the efficacy of a new product made of pongamia oil - pongamia oil soap at different concentrations in combating the major pests of okra viz., shoot and fruit borer, leaf roller and leaf hopper. Laboratory bioassay of pongamia oil soap was carried out in the Department of Agricultural Entomology, College of Agriculture, Padannakkad, during 2018-19 to evaluate the feeding deterrency and growth retardation properties of pongamia oil soap against fourth instar and first instar larvae of bhindi leaf roller, Sylepta derogata respectively and its insecticidal property or repellent property on bhendi leaf hopper, Amrasca biguttula biguttula. The test organisms were exposed to six treatments viz., T1: Pongamia oil soap 0.6%; T2: Pongamia oil soap 1%; T3: Pongamia oil soap 2%; T4: Neem oil soap 0.6%; T5: Soap solution 0.5%; T6: control with three replications under completely randomised design (CRD). Among the different treatments, pongamia oil 2 per cent showed the maximum antifeedent activity on fourth instar larvae and maximum growth retardation activity on first instar larvae of S. derogata. Spraying of pongamia oil soap 2 per cent showed 100 per cent mortality of leaf hoppers by 16 h after the treatment. But neem oil soap 0.6 per cent and pongamia oil soap 0.6 per cent took 24 h to attain 100 per cent mortality. Field efficacy of pongamia oil soap was evaluated by randomised block design (RBD) with seven treatments and four replications on okra variety ‘Arka Anamika’ during rabi and summer seasons at the Instructional farm in College of Agriculture, Padannakkad. The treatments applied were: T1: Pongamia oil soap 0.6%; T2: Pongamia oil soap 1%; T3: Pongamia oil soap 2%; T4: Neem oil soap 0.6%; T5: Soap solution 0.5%; T6: Quinalphos 0.05% - (Standard check); T7: Control. All treatments were applied once at vegetative stage and twice during reproductive stage. Observations were taken one day prior to treatment and 1, 3, 5, 7 and 14 days after treatment (DAT) for sucking pests. Damage symptoms caused by lepidopteran pests were observed one day prior to and 7 and 14 DAT. After three sprays during the rabi season pongamia oil soap 2 per cent was superior among botanicals in reducing the leaf hopper population significantly as compared to that of control. The effectiveness against leaf hopper lasted up to seven days after treatment. By the end of the rabi season pongamia oil 2 per cent showed significant reduction in damaged leaves as compared to other botanicals whereas the damage per cent was significantly increased in control and soap solution 0.5 per cent. Damage caused by leaf roller was cut down by the treatment of pongamia oil soap 2 per cent for seven days due to its antifeedent, repellent and growth retardation activity. Mean percentage of shoots and fruits infested by okra shoot and fruit borer Earias vitella and fruit borer Helicoverpa armigera during rabi and summer season was observed lowest in pongamia oil 2 per cent among botanicals. Reduction in mean per cent of fruit and shoot damage by pongamia oil soap may be due to the feeding deterrency of pongamia oil which remained effective for seven days. However all the treatments were effective in reducing the pest infestation except soap solution 0.5 per cent. Effectiveness of pongamia oil soap was observed to decline after seven days after spray application. Higher concentration of the soap gave better results as compared to that of lower concentrations. Pongamia oil soap 2 per cent exhibited increased fruit yield and marketable yield as compared to that of control due to lower incidence of pests during both the seasons. Economics of pongamia oil soap 2 per cent in turns of cost benefit ratio was also high as compared to control in both rabi and summer seasons, making it an effective component in IPM programmes and organic farming.ThesisItem Open Access Evaluation of micronutrient formulation in cowpea(Department of Soil Science and Agricultural Chemistry, College of Agriculture, Padannakkad, 2019) Roshni John; KAU; Binitha, N KThe project “Evaluation of micronutrient formulation in cowpea (Vigna unguiculata L. Walp)” was carried out at College of Agriculture, Padannakkad and Regional Agricultural Research Station (RARS), Pilicode during 2017 to 2019. The objectives of the study were to evaluate the effect of micronutrient formulation through seed treatment and foliar nutrition on growth, nodulation and yield of cowpea and to study its effect on plant nutrient uptake and residual soil nutrient status. Laboratory study was carried out at COA, Padannakkad to formulate and standardize the micronutrient solution for cowpea. Different concentrations of the standardized micronutrient formulation were used for seed treatment study (Experiment 1). The experiment was carried out in completely randomized design with 7 treatments and 3 replications which included seed treatment with micronutrient formulation @ 0.25, 0.50, 0.75, 1, 1.5 and 2 per cent as T1, T2, T3, T4, T5 and T6 respectively. T7 (seed treatment with water) was the control. Observations on germination percentage, number of days taken for germination, seedling length at three leaf stage and seedling vigour index were recorded. Treatment T6 (micronutrient formulation @ 2 %) showed highest seedling length (24.23 cm) and seedling vigour index (2423) which was followed by T5 (micronutrient formulation @ 1.5 %). Thus the best and second best treatment from seed treatment study was concluded as T6 and T5 respectively and was selected for the field experiment (Experiment 2). The field experiment was conducted in randomized block design with 12 treatments replicated three times. The treatment consisting of combination of four levels of seed treatment (no seed treatment, seed treatment with rhizobium, seed treatment with best concentration from experiment 1 and second best seed treatment from experiment 1) and three levels of foliar application of micronutrient (no foliar application, one foliar application at 15 DAS and two foliar applications at 15 and 30 DAS). Biometric observations were recorded at flower bud initiation and at harvest. Plant height at flower bud initiation and at harvest was superior in S3 (Seed treatment with micronutrient formulation at 2 %) while stem diameter was highest in S4 (seed treatment at 1.5 %) and S3 respectively in both stages. Double foliar spray was superior in plant height and stem diameter at both stages. Interaction S3F3 (2 per cent seed treatment plus two foliar spray) recorded highest plant height at both stages and stem diameter was highest in S2F3 (treatment with rhizobium plus two foliar spray). Number of nodules was highest in S2 (seed treatment with rhizobium). Number of pods per plant (21.02 & 20.97), pod weight per plant (36.14 g & 33.49 g), grain yield (2224 kg ha-1) and dry matter production (2292.91 & 2372.92 kg ha-1) was superior in S3 and F3 (two foliar spray). Interaction S3F3 was superior in number of pods per plant (25), pod weight per plant (38.11 g), grain yield (2320 kg ha-1) and dry matter production (2613.24 kg ha-1). Leaf analysis revealed that P and K content in leaf was highest in S3. N, P, K and Ca content in leaf was highest in F3. Interaction S3F3 recorded highest N, P and K content in cowpea leaf. Mg content was highest in S2F3 (treatment with rhizobium plus two sprays). Fe, Mn, Zn and B content in leaf was highest in S3 and F3. Interaction S3F3 (2 per cent seed treatment plus two foliar spray) recorded highest Fe, Mn and Zn content in cowpea leaves. The uptake of N, P, K, Ca, S, Fe, Mn, Zn and B were highest in both S3 and F3 whereas Mg uptake was highest in S3 and F2 (one foliar spray). Cu uptake was highest in F3. Interaction S3F3 recorded highest uptake of N, P, Mn and Zn. N content in cowpea grain was superior in S2 and F3. While K, Ca, Fe, Mn, Zn, Cu and B content in cowpea grain was highest in both S3, F3 and interaction S3F3. In case of interaction, N was highest in S2F3. The uptake of N, P, K, Ca, Mg, S, Fe, Zn and B in cowpea grain was highest in both treatments S3 and F3 and the interaction, S3F3 recorded highest uptake of P, K, Ca, Fe, Mn, Zn and B in grain. Studies on residual soil nutrient status revealed that available N and P in soil was highest in S2 at both 45 DAS and at harvest. Available K was highest in S3, F3 and S3F3 at both stages. Organic carbon content was found to increase in plots receiving two foliar spray and interaction S3F3. Available Fe, Mn and Zn was highest in S3 at harvest and in F3 at both stages. Available B was highest in S3 and F3 at 45 DAS while Mo at harvest. B and Zn were highest in S3F3 at 45 DAS while at harvest available Fe was found to be higher in S3F3. Available Fe at 45 DAS was highest in S1F3 (no seed treatment plus two foliar spray). Root CEC was superior in S3, F3 and in interaction S3F3. The results obtained from the investigation revealed that the application of micronutrient formulation as two foliar spray (15 and 30 DAS) along with seed treatment (2 per cent) was found to be highly effective in increasing the growth and yield characters of cowpea. The uptake of nutrients in cowpea leaf and grain was also found to increase with the application of micronutrient formulation enhancing the nutritional quality of grain.
