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2002 - 20104
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Subject: Veterinary Gynaecology and Obstetrics ×

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Now showing 1 - 4 of 4
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    ThesisItemOpen Access
    Hormonal Induction of Estrus and Cyclicity in Postpartum Anestrus Cows of Bos Indicus of Semi-Arid Region of Rajasthan
    (Department of Animal Reproduction Gynaecology and Obstetrics College of Veterinary and Animal Science Rajasthan Agricultural University, BIKANER-334001, 2008) RAJESH KUMAR CHOUDHARY; J.S. Mehta
    Various hormonal regimens were tried to ensue reproductive activity in prolonged postpartum anestrous cows of B. indicus. The cows were assigned as control (no hormonal treatment - inj. N.S. S/c O.D × 7d) and hormonal regimens as T1 (P – IVS – intravaginal sponge impregnated with 1.5 g progesterone for 7d); T2 (P – inj. progesterone – 150 mg s/c O.D × 7d); T3 (PE – inj progesterone – 150 mg s/c O.D × 7 d + inj. estradiol valerate 5 mg i.m. O.D on day 1); T4 (PeCG – inj progesterone – 150 mg s/c O.D × 7 d + inj. eCG – 400 I.U. i.m. O.D on d 7) and T5 (PEeCG – inj progesterone – 150 mg s/c O.D × 7 d + inj. estradiol valerate 5 mg i.m. O.D On d 1 + inj. eCG – 400 I.U. i.m. O.D on d 7).
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    ThesisItemOpen Access
    MORPHOLOGIC SURVIVAL AND SUBSEQUENT IN VITRO MATURATION OF DENUDED AND CUMULUS COMPACT BUBALINE OOCYTES CRYOPRESERVED BY ULTRA RAPID COOLING
    (Department of Animal Reproduction Gynaecology & Obstetrics College of Veterinary and Animal Science, Bikaner Swami Keshwanand Rajasthan Agricultural University, Bikaner – 334001 (Rajasthan), 2009) VINOJ KUMAR MEENA; Dr. G.N. PUROHIT
    Culturable grade oocytes (n = 380) recovered by aspiration of surface follicles from buffalo ovaries (n = 97) were either mechanically denuded (DN) or kept cumulus intact (CC) and were vitrified in DPBS + 0.4% sucrose, 0.4% BSA and 6 M concentrations of either ethylene glycol (EG) or propylene glycol (PG). Oocytes were randomly allocated to four groups i) EGCC ii) EGDN iii) PGCC and iv) PGDN of vitrification and cryostorage for 7-10 days in LN2. They were then warmed to record morphologic survival and morphologically normal oocytes were matured in vitro along with fresh oocytes (control) for 24 h in TCM-199 containing hormones (LH + FSH + estradiol) at 38.5 ± 1oC and 5% CO2 in humidified air in a CO2 incubator. The arcsine transformed data of the proportion of morphologic survival of oocytes and in vitro maturation of oocytes was compared by DNMR-test.
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    ThesisItemOpen Access
    VITRIFICATION OF IMMATURE AND IN VITRO MATURED CUMULUS COMPACT AND DENUDED GOAT OOCYTES AND THEIR SUBSEQUENT FERTILIZATION IN VITRO
    (Department of Animal Reproduction Gynaecology & Obstetrics College of Veterinary and Animal Science, Bikaner Swami Keshwanand Rajasthan Agricultural University, Bikaner, 2010) Harikesh Meena; Dr. G.N. PUROHIT
    The morphological survival, in vitro maturation and in vitro fertilization of cumulus compact immature (ImCC), and in vitro matured (MCC) and, denuded immature (ImDN) and denuded in vitro matured (MDN) goat oocytes vitrified using DPBS + 0.5% sucrose + 0.4% BSA with 8 M propylene glycol was studied in two separate experiments (Experiment 1 and 2) in order to evaluate the effect of cumulus cells and the meiotic stage at cryopreservation on subsequent oocytes development. After 7-10 days of cryostorage oocytes were rewarmed to record i) morphological survival (Experiment 1 and 2) ii) nuclear maturation (Experiment 1, 10 replicates) and iii) fertilization 24 h after inseminatioin with prepared epididymal buck sperms (Experiment 2, 8 replicates per group). The arcsin transformed data of the proportion of oocytes survived, matured or fertilized was compared by DNMR – test.
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    ThesisItemOpen Access