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ThesisItem Open Access Effect of botanicals on seed borne leaf crinkle virus in Black Gram (Vigna mungo L).(Mahatha Phule Krishi Vidyapeeth,Rahuri, 2017) Tikhe Dnyanev Ashok; Latake, S.B.The present investigation entitled “Effect of botanicals on seed borne Leaf Crinkle Virus in Black Gram (Vigna mungo L).” was undertaken with a view to find the per cent seed transmission of leaf crinkle virus through the infected black gram seeds to next generation, to evaluate the efficacy of botanicals in controlling the seed borne infection of ULCV in black gram and to find out the resistant reaction of promisingblack gram genotypes against Urdbean Leaf Crinkle Virus (ULCV) under field conditions. In symptomatology, first characteristic virus symptom appeared three to four weeks after sowing of the crop. The third trifoliate leaf developed symptoms by an increase in size and turning light green in colour. Approximately after a week the typical leaf crinkling became conspicuous. As the plants grew further, the extent of crinkling on the younger leaves was more pronounced. Considerable malformation of the inflorescence was observed in early infected plants. Some plants became bushy in appearance and remain green in the field even after the healthy plants attained senescence. Seeds collected from the ULCV infected plants from various eight locations were sown under glass house conditions and the per cent transmission for each sample was worked out. The rate of virus transmission through the infected seeds ranged between 40.23 to 53.66 per cent indicating that seed play an important role in transmission of ULCV. Eleven plant extracts were evaluated for their efficacy in controlling the seed borne infection of ULCV in black gram/ urdbean. Prior to sowing, seeds were dipped in the individual crude extracts of the plant species for 6, 12 and 18 hours and observations were recorded.
