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ThesisItem Open Access EFFECT OF AZOTOBACTER, ARBUSCULAR MYCORRHIZA ON THE GROWTH AND YIELD OF BRINJAL(MPKV, Rahuri, 2024) Wankhade Harish Prakash; Sonawane, R. B.The present investigation entitled, "Effect of Azotobacter, Arbuscular mycorrhiza on the growth and yield of brinjal" was conducted during kharif 2022-23 at an experimental farm of the Department of Plant Pathology and Agricultural Microbiology, Mahatma Phule Krishi Vidyapeeth, Rahuri. An experiment was laid out in a Randomized Block Design. Brinjal seedlings of variety (Phule Arjun) were treated with liquid broth of Azotobacter and Arbuscular mycorrhiza before transplanting with 7 treatments and 3 replications. Observations regarding growth, yield, Nutrient uptake and microbial count status were recorded. The maximum plant height at flowering (62.73 cm), at harvesting (94.33cm) and root length (45.15 cm). were recorded in treatment T2 i.e. 100% RDF + Azotobacter + Arbuscular mycorrhiza, The maximum Total number of fruit per plant (46.50), and maximum weight of fruits (21.86 t ha-1), fruit yield (42.98 t ha-1) were recorded in treatment T2 i.e. 100% RDF + Azotobacter + Arbuscular mycorrhiza. The treatment T2 recorded 32.81% increase in yield over control and % increase over 100% RDF.ThesisItem Open Access STUDIES ON SEED BIOPRIMING WITH BACTERIAL CONSORTIA IN GREENGRAM (Vigna radiata (L.) Wilczek)(MPKV, Rahuri, 2024) Kota Tejasree; Narute, T.K.The present investigation was carried out on “Studies on seed biopriming with bacetrial consortia in greengram (Vigna radiata (L.) Wilczek)” during Kharif season of the year 2022-23. It was conducted at the Post Graduate Institute Farm, Mahatma Phule Krishi Vidyapeeth, Rahuri, in randomized block design with three replications. The variety Phule Chetak of Greengram was used as a test crop along with 9 treatments of bacetrial consortia (Rhizobium, PSB and KMB) with different doses of NPK application. Treatments consisted of T1 i.e. Only seed biopriming with Rhizobium, PSB and KMB with no RDF of N, P2O5, K2O, T2 i.e. Seed biopriming as above with 50 % N, P2O5 and K2O ha-1, T3 i.e. Seed biopriming as above with 75% N, P2O5 and K2O of N, P2O5 and K2O ha-1 and T4 i.e. Seed biopriming as above with 100 % N, P2O5 and K2O of N, P2O5 and K2O ha-1), T5 i.e. Seed biopriming with reference strains of Rhizobium, PSB and KMB with 50 % RDF of N, P2O5 and K2O ha-1. T6 i.e. seed biopriming with reference strains of Rhizobium, PSB and KMB with 75% RDF of N, P2O5 and K2O T7 i.e. Seed biopriming with MPKV’s reference strains of Rhizobium, PSB and KMB with 100 % RDF of N, P2O5 and K2O. T8 i.e. Only seed biopriming with reference strains of Rhizobium, PSB and KMB with no RDF and T9 as an absolute control.ThesisItem Open Access EFFECT OF MPKV BACTERIAL CONSORTIUM ON GROWTH AND YIELD OF MAIZE(MPKV, Rahuri, 2024) Talekar Onkar Balasaheb; Kolase, S.V.The present investigation, titled “Effect of MPKV’s bacterial consortium on growth and yield of maize.” was conducted during kharif, 2022-23 at the All India coordinated research project on Maize, MPKV, Rahuri. This study involves isolating PGPR (Azotobacter, Bacillus and Pseudomonas) from rhizospheric soil of maize and testing each isolate for morphological, cultural and biochemical characteristics. For their functional potential Azotobacter was examined for nitrogen fixation activity, Bacillus for phosphate solubilization and Pseudomonas for potash mobilization activity respectively. Most efficient strains were used for field studies. Among all the 9 isolates, the strain (AAzt1, ABac2 and APdm3) showed highest N, P and K solubilizing ability in-vitro study were used to prepare consortium on compatible media which is finally further used for field studies. A field experiment was conducted to appraise their effect in combination with graded levels of nitrogen, phosphorous and potassium on growth, and yield of maize (cv. Phule Rajarshi). The field observations were taken on plant height, cob width, cob length, number of cobs/plants, 1000 grain weight, grain yield, nutrient uptake and microbial population.ThesisItem Open Access EVALUATION AND CHARACTERIZATION OF RHIZOSPHERIC MICRO-ORGANISMS ASSOCIATED WITH WILD XEROPHYTIC PLANTS ON SORGHUM(MPKV, Rahuri, 2023) Barge Monika Suresh; Narute, T.K.Laboratory studies were conducted at the Department of Plant Pathology and Agricultural Microbiology, Post Graduate Institute MPKV, Rahuri. Moreover, a field experiment was also conducted during Rabi 2021-22 and 2022-23 at the Department of Plant Pathology and Agricultural Microbiology, Post Graduate Institute MPKV, Rahuri on sorghum crop in Randomized Block Design with 7 treatments and 4 replications. Total 125 soil samples were collected during August 2021 to October 2021 from rhizospheric soils of xerophytic plants such as Ber (Ziziphus mauritiana), Alovera (Aloe barbadensis), Cactus (Cactus spp.), Opuntia (Opuntia ficus) and Agave (Agave americana) etc. from different villages of five districts of Maharashtra State viz., Kolhapur, Sangli, Satara, Pune and Ahmednagar in accordance with GPS location. Azotobacter were isolated on Jensen’s Agar Medium and Phosphate solubilizing bacteria were isolated on Pikovskaya’s Medium. These isolates were used for seed inoculation of sorghum a drought tolerant crop for which absolute no irrigation was given. These isolates were further characterized and identified upto species of microorganisms on the basis of morphological, biochemical, cultural, physiological characterization and Gram reactionThesisItem Open Access ROLE OF PLANT GROWTH PROMOTING RHIZOBACTERIA IN ABIOTIC STRESS CONDITIONS FOR THE TOLERANCE AND SURVIVAL OF TOMATO (Solanum lycopersicum L.)(MPKV, Rahuri, 2023) Satyam; Navale, A.M.The basic aim of the present study was to isolate salt and temperature tolerant rhizobacteria from rhizospheric soil of tomato and to see its effect on growth, nutrient uptake and yield of tomato crop under pot culture and field conditions. In the present study total one hundred thirty eight isolates were isolated from rhizospheric soil of tomato of Western Maharashtra. The classification of salt tolerant rhizobacteria was done on the basis of NaCl and MgCl2 percentage. Eighty-nine isolates out of one hundred thirty-eight had salt tolerance levels that exceeded the 5.4 % limit. Seven isolates could withstand salt concentrations of 2.10–3.6 % NaCl. Sixteen isolates showed moderate tolerance to NaCl concentrations (salt content of 0.9–1.5 %). Twentee six (26) isolates had a moderate sensitivity to salt concentrations of NaCl (0.15– 0.60 %). The salt tolerance limit was used to categorise salt-tolerant rhizobacteriaThesisItem Open Access EVALUATION OF MPKV’s BACTERIAL CONSORTIUM ON GROWTH AND YIELD OF TOMATO CROP(MPKV. Rahuri, 2023) BORHADE SHUBHAM MANIK; Navale, A.M.The present investigation entitled “Evaluation of MPKV’s bacterial consortium on growth and yield of tomato crop” was undertaken during rabi 2022, at experimental farm of Department of Plant Pathology and Agricultural Microbiology, Post Graduate Institute, Mahatma Phule Krishi Vidyapeeth, Rahuri to see the effect of bacterial consortium on growth, nutrient uptake and yield of tomato crop. The present investigation involved laboratory study and field experiment. In the laboratory study, Tomato rhizospheric soil was used to isolate Azotobacter, Bacillus and Pseudomonas strains, which were then cultured on Jensen's medium, Pikovaskaya’s medium and Aleksandrow’s medium and identified using morphological, cultural and biochemical characteristics. Azotobacter and Pseudomonas showed Gram negative reactions in morphological tests, while Bacillus showed Gram positive reactions. Azotobacter colonies were yellow, Bacillus colonies were white and Pseudomonas colonies were light green. All three bacteria, however, were 1-2 mm in size. Azotobacter produced dark brown, Bacillus produced yellowish white and Pseudomonas produced yellowish green pigmentation.ThesisItem Open Access STUDIES ON PINK PIGMENTED FACULTATIVE METHYLOTROPHS ON TOMATO (Lycopersicum esculentum L.) CROP(MPKV, Rahuri, 2023) Nakade Suchita Shashikant; Navale, A.M.After formulation of liquid consortia the shelf of PPFM formulation stored in UV sterilized high density polyethylene (HDPE) bottles were investigated for shelf life of consortium. Cell count was taken for monthly duration consecutively for upto 360 days. The maximum cell count was recorded at 180 days after storage and thereafter gently decreases upto 360 days. The field trial was carried out in summer (2022) to assess the Combined effect of PPFM and Azotobacter and various treatment combination on various plant growth parameters in tomato like plant height, number of leaves, root length, fresh weight and dry weight, number of branches, number of fruits, yield, nutrient uptake in chilli and population dynamics of PPFMs recorded at flowering and harvesting phase under graded level of nitrogen in field condition.ThesisItem Open Access SENSORY MICROBIAL TECHNIQUE TO DETECT PESTICIDES AND HEAVY METALS RESIDUES IN MARKETABLE FRUITS AND VEGETABLES(MPKV, Rahuri, 2023) Nadaf Jasmin Mansoor; Navale, A.M.The prevalence of harmful pesticide residues, heavy metals, as well as microbial contamination in fresh fruits and vegetables is a major public health problem across the globe. The purpose of this study was to assess the residual pesticide viz, Cypermethrin, Spinosad, Acephate, Carbendazim, as well as Chlorpyriphos, heavy metal viz., Lead, Cadmium and Cromium, as well as the microbial contamination quality and safety of common marketable fruits and vegetables samples from various local markets in Mumbai. The current study, named "Sensory microbial Technique to detect pesticide residues as well as heavy metals in marketable fruits and vegetables," was conducted at Mahatma Phule Krishi Vidyapeeth, Rahuri, Departments of Plant Pathology and Agricultural Microbiology.ThesisItem Open Access STUDIES ON EFFECT OF CLOVE INOCULATION OF DIFFERENT LIQUID BIOFERTILIZERS ON GRWOTH AND YIELD OF GARLIC(MPKV, Rahuri, 2023) Jamdhade Madan Balaji; Sonawane, R.B.The garlic (Allium sativum) is a common vegetable plant in the Amaryllidaceae family, which being cultivated worldwide for vegetable purpose. The garlic, commonly known vegetable, is the genus Allium's most frequently farmed species. The aim of the present study was to isolate Pseudomonas spp., Frateuria aurantia and Azotobacter spp. from the rhizosphere soils of garlic crop and to studied the clove inoculation of different liquid biofertilizers on growth parameters and yield of garlic.
