Thesis
Browse
Browsing Thesis by Subject "Agricultural Biochemistry"
Now showing 1 - 18 of 204
Results Per Page
Sort Options
ThesisItem Open Access Agricultural Biochemistry ical Analysis of pigeonpea cultivars differing in reaction to pod Borer damage.(Mahatma Phule Krishi Vidyapeeth, Rahuri, 1993) Murkute, G. R; Desai, B. B; 574.192/MURThesisItem Open Access Agricultural Biochemistry ical Investigation of Pomengranate Arils in Relation to blackening.(Mahatma Phule Krishi Vidyapeeth, Rahuri, 1993) Sawant, B. A; Desai, B. B; 574.192/SAWThesisItem Open Access Analysis of Pomegranate Peel Extract (PPE) For Antoxidant and Antimicrobial Properties(Mahatma Phule Krishi Vidyapeeth, Rahuri, 2014) Manapure, N. V.; Naik, R. M.; 574.192/MANThesisItem Open Access ANTIOXIDANT POTENTIAL OF POMEGRANATE PEEL AQUEOUS EXTRACTS AND SYNTHESIS OF SILVER NANOPARTICLES(MPKV, Rahuri, 2023) Dhakne Aishwarya Sudhakar; Bhalerao, B.M.Nanotechnology has marked a significant revolution in various areas of science and involved in the production and application of nanoparticles that can be used in different fields. Silver nanoparticles among other nanoparticles, have received substantial attention because of their unique properties. This study focuses on the green synthesis of silver nanoparticles using pomegranate peel extracts. Green synthesized silver nanoparticles have great advantages, including energy efficiency, low toxicity, high yields, cost-effectiveness, eco-friendliness, and ready availability. The Antioxidant potential of pomegranate peel aqueous extracts and synthesis of silver nanoparticles was carried out in Department of Biochemistry, Mahatma Phule Krishi Vidyapeeth, Rahuri. In the present study total five different solvents in order to polarity were used namely water, ethanol 60%, methanol 60%, ethyl acetate and petroleum ether respectively. In the extraction process solvent selection was a very important step because the solubility of required compounds was depend upon the polarity of the solvent. Method of extraction is widely affect the yield of the extracts. In this study four different extraction methods were used viz ultrasonic assisted extraction (UAE), soxhlet extraction (SE), microwave assisted extraction (MAE), and maceration extraction (ME) for comparison. The earlier scientists reported that pomegranate peel has high antioxidant activity than its flower, leaf and seed. The pomegranate peel extracts act as a natural inhibitors of plant pathogens like fungi and bacteria. In the present study Bhagwa, Superbhagwa and Ganesh pomegranate cultivar peels as agro waste material were used for comparative antioxidant study.ThesisItem Open Access ASSESSMENT OF BIOCHEMICAL AND MOLECULAR MARKERS IN DIFFERENTIATING SUGARCANE GENOTYPES FOR SALT TOLERANCE(MPKV,Rahuri, 2010) MAHAJAN SUCHETA TAPIRAM; R.M.NaikSugarcane (Saccharum officinarum L.) crop shows high sensitivity to salinity at various growth stages. T.he present investigation entitled, Assessment of biochemical and molecular markers in differentiating sugarcane genotypes for salt tolerance" was undertaken with a view to analyse the effect of salt stress on different sugarcane genotypes. The sets of sugarcane were planted in normal soils. Then after one !TIOnth, the seedlings were transplanted to bigger earthen pots in normal, aline and sadie soils. The leaves from 75 days old plants were excised or the estimation of various biochemical parameters viz., proline, glycine etaine,soluble proteins, in vivo NR activity and the activity of PSCS. f urther,the leaf soluble proteins were extracted from sugarcane leaves grown on normal, saline and sadie soils and resolved on SDS-PAGE. The ifferences in isozyme forms of peroxidase and esterase were also bserved. The genomic DNA' isolated and purified from the sugarcane enotypes was amplified using different random primers.The leaf free proline content of all the sugarcane varieties increased_, the mean proline accumulation fFom 99.97 to 114.43 J..1 mole g-1 fr.wt. in saline soil and from 99.97 to 122.76 J..1 mole g-1 fr.wt. m sorlic soil. Comparison of the leaf prelme €ontents wblen gFOwn on normal, saline and sodic soils revealerl ~ferenees m feld increase in proline. Sugarcane genotypes CoM-0265,Co:-740 and Co-62175 showed an increase in proline content of 1.26, 1.25 and 1.22 .. £old in sa1ine soil and 1.34, 1.49and 1.28 -fold in sodic S0ilS, respectively. Amongst the promising clones, CoM -08011 appeared to 1De sa1t tolera.I'lt showing an increase in leaf proline content of 1.1 ~and 1.25 fold under salline and sodic conditions. It thus appears that fold increase in pF®line level is a sensitive parameter while evaluating salt toleFance and pFoline accumulatoin is an adaptive parameter unde:r stress c0naition. The early maturing and high sugar genetypes viz., CoC-671 and Co-940 12 appeared to be salt susceptible. ·A significant increase in mean g~cine ,betaine content was recorded with 4.34-fold increase in saline soils and 16.63 fold in sodic soils. A maximum fold iiacrease in glyci~e betaine content of 10.44 and 51.06 fold was reco~ded in CoM-0265, followed by Co-7 40 with a fold increase of 8.19 and 27.28 in saline and sodic soils~ respectively . The early maturing aE.d nigfu suga:r centaining sugarcane genotypes,.,CoC-671 and Co-94012 showed the minimum fold increase of 2.45 and 1.68, respectively when grown on saline soils and 7.42 and 6.77 when grown on sodic soils.The soluble protein content also increased under stress aE.dWlehighest fold increase ef 2.52 and 4.18 was observed in CoM-0265 in saline and sodic soils,respeetively. 1'he increase in free proline content is not due te proteolysis but rather due to increased de-novo synthesis. The in vivo leaf nitrate reductase activity was drastically reduced when grown on saline and sodie soils. A :m.inimum reduction in the in vivo NR activity from 0.62 in normal soH to 0.54 J..1 mole N02~ fromed g-1 fr.wt.hr-1 in saline soil was recorded in CoM-0265, followed by a reduction of 14.46 % in Co-740. It is interesting to note that early aturing and high sugar genotypes, CoC-671 and Co-94012 showed igher in vivo NR activity of 1.63 and 1.61 j..1 mole N02- formed g -~ r.wt.hr-1 , respectively when grown on normal soils. However , the eduction in the in vivo NR activity was significant to the extent of 32.90 Yo in CoC-671 and 43.52% in Co-94012 in saline soils and 62.64% and 4.66% in sodic soils.ThesisItem Open Access ASSESSMENT OF FOXTAIL MILLET GENOTYPES IN RELATION TO NUTRITIONAL PERSPECTIVE(MPKV, Rahuri, 2024) Kadale Pallavi Balu; Kale, A.A.The antioxidant properties such as total phenol, bound phenol, free phenol, and DPPH scavenging activity of thirty foxtail millet genotypes were evaluated. The genotype KIFXG-22-13 was recorded having significantly higher total phenol (3.56 mg/g), bound phenol (2.22 mg/g), free phenol (1.34 mg/g) and DPPH scavenging activity (47.51 %). The mineral composition of thirty foxtail millet genotypes evaluated for iron, zinc, calcium, sodium and potassium. Among all the foxtail millet genotypes KIFXG-22-13 was found to be significantly superior in iron content (4.19 mg/100g), zinc content (4.10 mg/100g), calcium content (31.03 mg/100g), sodium content (5.80 mg/100g) and potassium content (270.50 mg/100g).ThesisItem Open Access Assessment Of Juice Quality In Sweet Sorghum (Sorghum Bicolor (L) Moench) Varieties(Mahatma Phule Krishi Vidyapeeth, Rahuri, 1989) Jadhav, M. M.; Chougule, B. A.; 574.192/JADThesisItem Open Access Assessment of Nitrate reductase activity and carbon monoxide sensitvity of cytochrome c oxidase in the leaflates of some chickpea cultivars.(Mahatma Phule Krishi Vidyapeeth, Rahuri, 1995) Waman, S. L; Munjal, S. V; 574.192/WAMThesisItem Open Access Assessment of nitrate reductase potential and carbon monoxide in the leaves of cytolasmic male sterile and maintainer seedlings of pearl milelt Pennisetum glaucum L.(Mahatma Phule Krishi Vidyapeeth, Rahuri, 2000) Pawar, P.N.; Munjal,S.V.; 574.192/PAWThesisItem Open Access Assessment of Nutritional Quality of Forage F1 Bajra x Napier Hybrids(Mahatma Phule Krishi Vidyapeeth, Rahuri, 2017) Gate Dnyaneshwar V.; Damame, S.V.; 574.192/GATThesisItem Open Access Assessment of protein and limiting amino acids content of grains and biochemical markers associated with drought tolerance in chickpea genotypes(MPKV, Rahuri, 2010) Gosavi Sachin Prabhakar; Lokhande, P.K.In conclusion, the drought tolerant genotypes such as PG-96006, Annegeri and Vijay with comparatively higher potential to accumulate proline and glycine betaine and the expression of unique prote~ bands in them could be further exploited for introgression of drought tolerance character in the existing germplasm known to have better yield and protein accumulating attributes.ThesisItem Open Access Assessment Of Protein, Lysine & Starch Contents In Grains & Agricultural Biochemistry ical Markers Associated With Drought Tolerance In Sorghum Genotypes(Mahatma Phule Krishi Vidyapeeth, Rahuri, 2010) Patil, S.D.; Damame, S. V.; 574.192/PATThesisItem Open Access ASSESSMENT OF PROTEIN, LYSINE AND STARCH CONTENTS IN GRAINS AND BIOCHEMICAL MARKERS ASSOCIATED WITH DROUGHT TOLERANCE IN SORGHUM GENOTYPES(MPKV,Rahuri, 2010) PATIL SUNIL DILIPRAO; S. V. DAMAMEThe present investigation was undertaken to evaluate the ~ levels of crude protein, lysine and starch from the sorghum grains of seven each of drought tolerant and susceptible genotypes. In addition, the levels of proline, glycine betaine, the activity of PSCS and soluble proteins were determined from the leaves of these genotypes by imposing stress created by polyethylene glycol-6000. Among the drought tolerant genotypes under study, the highest crude protein content of 9.63% was observed in the grains of RSV -962, whereas among the drought susceptible ones, the highest of 9.24% was observed in CSV-216. Amongst the drought tolerant genotypes, the highest lysine content of 2.19 g 16 g-1 N was observed in the grains of RSV-458 and among the susceptible ones, the highest of 2.17 g 16g-1 N was observed in SPV-504. As regards the starch content, a drought tolerant genotype SPV-1546 recorded the highest of 77.40 per cent in the grains, whereas among the drought susceptible ones, RSLG-1119 recorded the highest of 76.50 per cent. The seedlings of 2-weeks-old sorghum genotypes were evaluated for the accumulation of free proline, glycine betaine and P5CS activity under control and PEG induced stress of -0.5 MPa. On imposition of stress the mean proline accumulation increased by 7 -folds in drought tolerant genotypes as compared to a 3-folds increase in drought susceptible ones. The highest proline content of 544.4 pmol g-I fr.wt. was recorded in a drought tolerant genotype, RSLG-262. The activity of the rate-limiting enzyme, P5CS in leaf materials of drought tolerant genotypes demonstrated correspondingly higher activity than the susceptible ones under PEG induced stress. The mean fold increase of 2.44 in P5CS activity of leaf materials was observed in drought tolerant genotypes, whereas there was 1.44-fold in drought susceptible ones. The maximum fold increase of 3.42 in P5CS activity was observed in a drought tolerant genotype, Sel-3. As regards the accumulation of glycine betaine, the mean fold increase of 3.71 was observed in drought tolerant genotypes as compared to 1.57 -fold in leaf materials of a drought susceptible ones.ThesisItem Open Access Assessment Of Some Chemical Parameters Responsible For Shootfly Resistance In Sorghum(Mahatma Phule Krishi Vidyapeeth, Rahuri, 1991) Bhise, H.T.; Desai, B. B.; 574.192/BHIThesisItem Open Access Bio- Chemical Changes In Onion Bulbs (Allium Cepa L) During Storage(Mahatma Phule Krishi Vidyapeeth, Rahuri, 1987) Marmat, K. R.; Kadam, S. S.; 574.192/MARThesisItem Open Access Bio-Chemical Changes In Proteins And Lipids During Storage Of Heat Processed Groundnut (Arachis Hypogaea L)(Mahatma Phule Krishi Vidyapeeth, Rahuri, 1988) Damame, S. V.; Chavan, J. K.; 574.192/DAMThesisItem Open Access Bio-Chemical Investigations On Jack Bean Seeds : Solubilization Of Proteins And Effects Of Heat Treatments And Germination On Trypsin Inhibitor Activity And Polyphenols(Mahatma Phule Krishi Vidyapeeth, Rahuri, 1984) Babar, V. S.; Kadam, S. S.; 574.192/BABThesisItem Open Access Bio-Chemical Investigations On Preharvest Seed Germination In Groundnut (Arachis, Hypogaea L)(Mahatma Phule Krishi Vidyapeeth, Rahuri, 1984) Pandit, S. V.; Kadam, S. S.; 574.192/PAN