Anand Agricultural University, Anand
Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur
AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.
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Browsing Anand Agricultural University, Anand by Subject "Agricultural Microbiology"
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ThesisItem Open Access AGRICULTURALLY BENEFICIAL BACTERIAL ISOLATES FROM PANCHAGAVYA AND ESTABLISHING THEIR PGP ROLE IN CHILLI (Capsicum annuum L.)(DEPARTMENT OF AGRICULTURAL MICROBIOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND, 2016) Vaibhav Vinaykumar Panchal; Prof. Harsha N. ShelatThe present study was aimed to prepare Panchagavya (PG) which is concoction of desi cow’s direct (Milk, cow dung, cow urine) and 2 derived (curd and ghee) products. Moreover, Modified Panchgavya (MPG) using additional ingredients like sugarcane juice, yeast and ripened banana was also prepared. Total ten bacteria were isolated using different nutrient media from PG and MPG. The isolates were characterized morphologically and biochemically. Amongst all, two isolates V1PG, V3PG isolated from Panchagavya and two isolates V9MPG and V10MPG proved to be the best for their plant growth promoting efficiency viz. solubilization/mobilization of insoluble phosphate and potash, production of siderophore, cellulase and ACC deaminase enzyme and growth hormone - indole acetic acid (IAA). All the four bacteria showed inhibition of plant pathogenic fungi however, isolate V1PG proved the best and shown zone of inhibition of funagl pathogens Fusarium oxysporum, Macrophomina phaseolina Fusarium solani, Aspergillus niger, Pythium sp. and Alternaria alternata by 50, 45 and 40, 40 , 21 and 20 percent followed by V3PG by 46, 35 , 36, 35, 20 and 15 percent , respectively over control.ThesisItem Open Access “AGRICULTURALLY BENEFICIAL BACTERIAL ISOLATES FROM PANCHAGAVYA AND ESTABLISHING THEIR PGP ROLE IN CHILLI (Capsicum annuum L.)”(Anand Agricultural University, Anand, 2016) Vaibhav Vinaykumar Panchal; Prof. Harsha N. ShelatThe present study was aimed to prepare Panchagavya (PG) which is concoction of desi cow’s direct (Milk, cow dung, cow urine) and 2 derived (curd and ghee) products. Moreover, Modified Panchgavya (MPG) using additional ingredients like sugarcane juice, yeast and ripened banana was also prepared. Total ten bacteria were isolated using different nutrient media from PG and MPG. The isolates were characterized morphologically and biochemically. Amongst all, two isolates V1PG, V3PG isolated from Panchagavya and two isolates V9MPG and V10MPG proved to be the best for their plant growth promoting efficiency viz. solubilization/mobilization of insoluble phosphate and potash, production of siderophore, cellulase and ACC deaminase enzyme and growth hormone - indole acetic acid (IAA). All the four bacteria showed inhibition of plant pathogenic fungi howeverThesisItem Open Access Azotobacter chroococcum (ABA-1) formulations, their shelf life and effect on growth of pearl millet(Anand Agricultural University, Anand, 2007) Acharya Payal Kaushikkumar; H. N. ShelatDifferent Liquid, Carrier and Granular formulations of Azotobacter chroococcum (ABA-1) were prepared from the Jensen’s broth having initial count 1010. Their in vitro survival study on solid Jensen’s medium as well as on bajra seeds and their in vivo efficacy testing on growth parameters of Pearl millet cv GHB-558 were conducted by seed and soil application in pots in Net house, Department of Microbiology, B.A.C.A, Anand Agricultural University, Anand, during 2006-07.ThesisItem Open Access BIODIVERSITY OF PLASTIC ADORING MICROORGANISMS AT ANAND AND THEIR APPRAISAL ON BIODEGRADABLE POLYETHYLENE(Anand Agricultural University, 2008) Kushwah Poonam Ajaysingh; Dr. R.V. VyasStudies on isolation of native plastic adoring beneficial microorganisms from landfills surrounding Anand in three different geographical niches were carried out following testing of their ability to degrade biodegradable polyethylene in laboratory by liquid flask culture and solid plate techniques. Bio-efficacy of microorganisms was also studied in pots under net house condition on biodegradable plastic and amylase activity of proven microorganisms in laboratory at Anand Agricultural University, Anand, during 2005 through 2007.The salient findings of the research are as followsThesisItem Open Access Characterization of indigenous Rhizobium with their PGPR qualities and effect on green gram (Vigna radiate L.)(AAU, 2014) Bhatt, Shraddha Bipinbhai; Dr. R. V. VyasThesisItem Open Access “CHARACTERIZATION OF NATIVE Bacillus thuringiensis FROM SOILS OF MIDDLE GUJARAT(Anand Agricultural University, Anand, 2007) H.K.Patel; Dr. H. G.VyasBacillus thuringiensis (Bt) is a gram-positive, spore forming, soil bacterium. Various strains of Bacillus thuringiensis produce different insecticidal crystal proteins which are encoded by cry genes. The present investigation was undertaken with the aim of characterizing native Bacillus thuringiensis isolates from Middle Gujarat with respect to identify the cry genes present in the native population and bioassay of the most potent isolateThesisItem Open Access CONSORTIUM DEVELOPMENT FROM PHYLLOSPHERIC AND RHIZOSPHERIC METHYLOTROPHIC BACTERIA OF PADDY AS LIQUID PLANT PROBIOTICS AND ITS EFFICACY ON CV. GURJARI(DEPARTMENT OF AGRICULTURAL MICROBIOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND, 2017) Prajapati Ronakkumar Rameshbhai; Dr. R. V. VyasPhyllospheric methylotrophic bacteria were isolated from leaf surface of paddy from Anand, Thasra and Nawagam Tehshil on NMS medium supplemented with methanol (1%) by Leaf imprinting technique. Three native phyllospheric efficient methylotrophic bacterial isolates (M 3, M 10 and M 15) selected for detailed studies and found compatible with three rhizospheric methylotrophic bacteria (AAU reference strains) viz. Bacillus aerius AAU M 8, Panibacillus illinoisensis AAU M 17 and B. megaterium AAU M 29, and were used for consortium development to test its efficacy on paddy cv. Gurjari. Screening and characterization through 16S rRNA sequencing was carried out to identify three selected isolates.ThesisItem Open Access CONSORTIUM DEVELOPMENT FROM PHYLLOSPHERIC AND RHIZOSPHERIC METHYLOTROPHIC BACTERIA OF PADDY AS LIQUID PLANT PROBIOTICS AND ITS EFFICACY ON CV. GURJARI(AAU, Anand, 2017) PRAJAPATI RONAKKUMAR RAMESHBHAI; Dr. R. V. VyasPhyllospheric methylotrophic bacteria were isolated from leaf surface of paddy from Anand, Thasra and Nawagam Tehshil on NMS medium supplemented with methanol (1%) by Leaf imprinting technique. Three native phyllospheric efficient methylotrophic bacterial isolates (M 3, M 10 and M 15) selected for detailed studies and found compatible with three rhizospheric methylotrophic bacteria (AAU reference strains) viz. Bacillus aerius AAU M 8, Panibacillus illinoisensis AAU M 17 and B. megaterium AAU M 29, and were used for consortium development to test its efficacy on paddy cv. Gurjari. Screening and characterization through 16S rRNA sequencing was carried out to identify three selected isolates. Beneficial native phyllospheric methylotrophic bacteria have additional ability to promote plant growth through one or more mechanisms (PGPR traits) viz. phosphorus and potash solubilization; ii production of phytohormones like indole-3-acetic acid (IAA) and siderophores production; tolerance to high pH and protection against pathogenic fungi as antagonist and possessing nifH gene for nitrogen fixation. Production of useful enzymes by isolates like methane degradation sMMO and MDH, as well as lipase and protease were confirmed in the laboratory. Plant growth promotion activity of phyllospheric methylotrophic bacterial isolates was studied in vitro through seed treatment of rice seeds were found to improve germination with better root and shoot. The experiment laid out in Randomized block design with four replication at Agricultural Research Station for irrigated crops, Thasra during 2014 and 2015. The treatments comprised of (T1: 100% RDF, T2: 80% RDF, T3: 60% RDF, T4: 80% RDF + consortium, T5: 60% RDF + consortium, T6: Only consortium and T7: Absolute control). The results indicated that T4 treatment showed significantly higher growth parameters viz., plant height, chlorophyll content, number of effective tiller/plant, root length, shoot fresh weight, shoot dry weight, yield attributing component viz. number of penicle/hill, length of penicle, number of grain/penicle, grain weight, grain yield, straw yield and higher methylobacterium count with better net return and saving of NPK chemical fertilizers (20%) followed by T1, T2 and T5 treatments over control.ThesisItem Open Access CONSORTIUM DEVELOPMENT FROM PHYLLOSPHERIC AND RHIZOSPHERIC METHYLOTROPHIC BACTERIA OF PADDY AS LIQUID PLANT PROBIOTICS AND ITS EFFICACY ON CV. GURJARI(AAU, Anand, 2017) PRAJAPATI RONAKKUMAR RAMESHBHAI; Dr. R. V. VyasPhyllospheric methylotrophic bacteria were isolated from leaf surface of paddy from Anand, Thasra and Nawagam Tehshil on NMS medium supplemented with methanol (1%) by Leaf imprinting technique. Three native phyllospheric efficient methylotrophic bacterial isolates (M 3, M 10 and M 15) selected for detailed studies and found compatible with three rhizospheric methylotrophic bacteria (AAU reference strains) viz. Bacillus aerius AAU M 8, Panibacillus illinoisensis AAU M 17 and B. megaterium AAU M 29, and were used for consortium development to test its efficacy on paddy cv. Gurjari. Screening and characterization through 16S rRNA sequencing was carried out to identify three selected isolates. Beneficial native phyllospheric methylotrophic bacteria have additional ability to promote plant growth through one or more mechanisms (PGPR traits) viz. phosphorus and potash solubilization; ii production of phytohormones like indole-3-acetic acid (IAA) and siderophores production; tolerance to high pH and protection against pathogenic fungi as antagonist and possessing nifH gene for nitrogen fixation. Production of useful enzymes by isolates like methane degradation sMMO and MDH, as well as lipase and protease were confirmed in the laboratory. Plant growth promotion activity of phyllospheric methylotrophic bacterial isolates was studied in vitro through seed treatment of rice seeds were found to improve germination with better root and shoot. The experiment laid out in Randomized block design with four replication at Agricultural Research Station for irrigated crops, Thasra during 2014 and 2015. The treatments comprised of (T1: 100% RDF, T2: 80% RDF, T3: 60% RDF, T4: 80% RDF + consortium, T5: 60% RDF + consortium, T6: Only consortium and T7: Absolute control). The results indicated that T4 treatment showed significantly higher growth parameters viz., plant height, chlorophyll content, number of effective tiller/plant, root length, shoot fresh weight, shoot dry weight, yield attributing component viz. number of penicle/hill, length of penicle, number of grain/penicle, grain weight, grain yield, straw yield and higher methylobacterium count with better net return and saving of NPK chemical fertilizers (20%) followed by T1, T2 and T5 treatments over control.ThesisItem Open Access “DETECTION OF nifH GENE, PRELIMINARY CHARACTERIZATION BY ARDRA AND PROTEIN PROFILING OF NATIVE DIAZOTROPHS AZOTOBACTER, ACETOBACTER AND AZOSPIRILLUM”(Anand Agricultural University, Anand, 2012) PRAJAPATI RONAKKUMAR RAMESHBHAI; Dr. R. V. VyasThe native diazotrophic bacterial isolates (ABA-1, ABA- 10, ABA-14, ABA-2010, ACG-2 and ASA-1) and four reference strains (MTCC-446 and MTCC-124 (Azotobacter chroococcum), MTCC-1226 (Acetobacter diazotrophicus) and MTCC-2306(Azospirillum lipofarum) were used for the study. Bacterial culture were revived on their specific nitrogen free medium. All diazotrophic bacteria were characterized by Amplified ribosomal DNA restriction analysis (ARDRA), detection of nifH gene by polymerase chain reaction (PCR) and whole cell protein profiling by SDS-PAGE. Phylogenetic analysis of 16S rDNA indicated that these diazotrophic bacterial isolates belonged to respective genus Azotobacter, Acetobacter and Azospirillum and closely related to standard strains. Whole cell protein profiling by SDS-PAGE showed the specific polypeptide band of Fe protein (60 kDa) responsible for nitrogenase enzyme in all native nitrogen fixing bacterial isolates as well as standard cultures viz., (MTCC-446 and 124), (MTCC-1226) and (MTCC-2306) at threeThesisItem Open Access DEVELOPMENT OF AZOLLA BASED PGPB BIOGRANULES AND ITS EFFICACY IN OKRA(DEPARTMENT OF AGRICULTURAL MICROBIOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND, 2018) Meghaben Rajeshbhai Patel; Mrs. H. N. ShelatPresent investigation was carried out during 2016 to 2017 with an aim to develop Azolla based PGPB biogranules and ascertain their shelf life followed by efficacy in okra cv Gujarat Okra 5 (Abelmoschus esculentus (L.) Moench). Mass production of Azolla was studied in various growth media viz.cow dung, H40, IRRI, RP, SSP etc. with / without soil and it was observed that addition of soil in various nutrient media was beneficial registering higher biomass. In comparison to all media, cow dung with soil was found better for long term higher yield of Azolla.ThesisItem Open Access Development of Azospirillum liquid bio-inoculants fortified with micronutrients and their assessment on tomato (Lycopersicon esculentum L.)(AAU, Anand, 2013) GUPTA, DIPMALA GOPALBHAI; VYAS, R. V.Present research was carried out to isolate efficient Azospirillum cultures from soil samples of two different locations practicing organic farming viz. Kathwada (Ahmedabad) and Ravipura (Anand). Three strains were screened out from two locations soil. All three native Azospirillum isolates were characterized based on their morphological, biochemical and physiological nature. Isolates As-1, As-2 and As-3 were found gram negative, curved motile rods with optimum pH range 4 to 8 and salt tolerance up to 5 % NaCl concentration. In vitro compatibility of Azospirillum isolates with micronutrients was assessed through qualitative 100 ppm and quantitative tests recommendation dose concentration of micronutrients indicated compatibility with all isolates. In vitro studies on PGPR traits of native potential Azospirillum isolates demonstrated that nitrogen fixing potentiality of these isolates were ranged from 11.1 to 25.0 mg N2 fixed/g of sucrose consumed. Estimation of phosphate solubilization capacity in the liquid medium revealed that all the strains released P from tri calcium phosphate (TCP) and isolate As-3 recorded maximum soluble phosphorous (20.8 µg/ml) at 3 DAI. Among isolates, As-1 and ASA-1 (standard strain) are able to solubilize zinc oxide. lAA production was recorded by all three isolates of Azospirillum at 5 DAI, ranged from 0.30 to 25.85 µg/ml lAA in tryptophan supplemented media. Isolates As-2 and standard ASA-1 showed ACC deaminase enzyme production. Isolate As-1 and As-3 produced yellow-orange color zone on CAS agar plate showing siderophore production. Among the tested isolates, As-2 found to possess cellulase, lipase and chitinase enzyme, while isolate As-1 was found to produce cellulase and lipase enzyme, whereas As-3 showed lipase and chitinase enzyme and among standard strain ASA-1 showed lipase, protease and chitinase activity. Isolate As-1 showed inhibition of Aspergillus and Fusarium and As-2 showed inhibition of all tested plant pathogenic fungi and isolate As-3 inhibited M. phaseolina and standard strain ASA-1 showed inhibition of Fusarium and M. phaseolina. All isolates showed varied results for PGPR activity. Isolate As-1 showed 69% PGPR activity, As-2 has 77% and As-3 has 54% and standard strain ASA-1 has 77 % activity. Cyst based formulation of Azospirillum isolates fortified with micronutrients at desired concentrations showed self-life up to 12 months of storage with 10 to power 8 cell cfu/ml. In laboratory assay on tomato, all native Azospirillum isolates and standard checks showed better growth over un-inoculated control. In pot experiment isolate As-2 along with micronutrient + 75 % RDF was found better to promote different growth parameters of tomato by seed and soil application methods during Kharif 2011 and 2012. Among the application methods, seed application showed better performance. In nursery experiment, also the isolate As-2 along with micronutrient + 75% RDF was found better to promote different growth parameters of tomato during Kharif 2011 and 2012. Micronutrient analysis of plant showed that, mobilization of micronutrient occurred in tomato seedlings when treated with Azospirillum + micronutrients and also showed 25 % possible saving of 'N' fertilizer. Entire consequence of experimentations signifies that, Azospirillum isolate As-2 possesses multiple plant growth promotion traits with best potency on tomato pots and nursery and also found to mobilize micronutrients in tomato seedlings.ThesisItem Open Access DIAZOTROPH ISOLATION AND nifH GENE EXPRESSION FROM RHIZOSPHERE OF RICE (Oryza sativa L.)(Anand Agricultural University, Anand, 2016) ARMI R. PATEL; Dr. R. V. VyasThe study of free-living nitrogen-fixing diazotrophs in soil is important in respect to the diversity of soil microbial communities. The present investigation was carried out to isolate efficient diazotrophic cultures from rhizospheric soil of organically and conventionally grown rice fields of Main Rice Research Station, Anand Agricultural University, Nawagam and study of nifH expression of the diazotrophs followed by microbial diversity studiesThesisItem Open Access Diversity of native rhizospheric fluorescent Pseudomonas and their biocontrol potential against some soil borne fungal pathogens(AAU, Anand, 2014) PANPATTE; Harsha N. ShelatPseudomonas fluorescens is an effective bacterial antagonists for the management of soil borne fungal pathogens because of its ability to rapidly colonize the roots of host plants and thus compete with soil borne pathogens for space and food. Production of secondary metabolites like antibiotics, Fe chelating siderophores and cell wall degrading enzymes are most often associated with suppression of fungal pathogens by P. fluorescens in the rhizosphere of several crops.ThesisItem Open Access EFFICACY OF NATIVE PGPR CONSORTIUM AND FORTIFIED FORMULATIONS BY PHYTOEXTRACTS FOR MANAGEMENT OF SOIL BORNE DISEASE COMPLEX WITH SPECIAL EMPHASIS ON ROOT KNOT NEMATODE IN CUCUMBER (CUCUMIS SATIVUS L.)(DEPARTMENT OF AGRICULTURAL MICROBIOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND, 2017) Panpatte Deepak Gopalrao; Mrs. H. N. ShelatResearch was carried out to develop fortified formulation comprising of bacterial strains Providencia vermicola AAU PR1 (NCBI ACCN: KJ161325), Pseudomonas putida AAU PR2 (NCBI ACCN: KJ161326) and Pseudomonas fluorescens AAU PR3 (NCBI ACCN: KJ161327) with extracts of botanicals viz. A. indica (neem), I. carnea (besharam) and B. juncea (mustard cake) having biocontrol activity. Individual bacterial strains were found compatible with each other as well as phyto-extracts up to 10% aqueous concentration; rather there was an increase in population of bacterial count in presence of phyto-extracts. Fortified PGPR consortium was prepared having initial bacterial count 1.3 x109 and its longevity studied for 12 months maintaining count up to 7.3 x 107 cfu/ml.ThesisItem Open Access EFFICACY OF PESTICIDE DEGRADING NATIVE BACTERIA AS PGPR IN MAIZE (Zea mays L.)(DEPARTMENT OF AGRICULTURAL MICROBIOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND, 2021) Patel Tasvirkumar Manubhai; Dr. H. K. PatelThe study was conducted with the aim to isolate and characterize the plant growth promotion activity of chlorpyrifos, profenofos and atrazine degrading native bacteria. Soil samples were collected from different research farms of Anand Agricultural University, Anand having history of pesticide application. Total 21 bacterial isolates were obtained following enrichment isolation technique from soil samples and screened for their pesticide degradation efficiency. Total 6 isolates were found effective for chlorpyrifos degradation, 6 for profenofos and 9 were found effective for the degradation of atrazine. Among these isolates, 2 best isolates were selected for each pesticide on the basis of their ability to utilize respective pesticide at higher concentration as sole source of carbon and energy. Based on results of the polyphasic characterization isolate CN1 was identified as Pseudomonas nitroreducens AAUCP 1 and CN 3 as Pseudomonas alcaligenes AAU CP 3 fond to degrade chlorpyrifos effectively; profenofos degrader isolate PN3 was identified as Pseudomonas aeruginosa AAUPF 3 and P3 as Pseudomonas stutzeri AAUPF 2; atrazine degrading isolates A2 identified and designated as Paenarthrobacter ureafaciens AAUATR 2 and isolate AN4 as Pseudomonas taiwanensis AAU ATR 4.ThesisItem Open Access FIELD EFFICACY OF LIQUID FORMULATION OF Azotobacter chroococcum AND Azospirillum lipoferum ON BRINJAL (Solanum melongena L.)(Anand Agricultural University, Anand, 2007) M.M.Saiyed; Dr. M. S. VoraThe present investigation was carried out with a view to examine field efficacy of liquid nitrogenous biofetilizers on growth, quality and yield of brinjal. Field experiment was laid out in Randomized Block Design with three factors based various treatment combinations i.e. liquid inoculants (I), nitrogen levels (N) and method of application (M). Plots having no inoculants were taken as controlThesisItem Open Access FUNGAL BIOFERTILIZERS FOR PREPARATION OF P AND K RICH ORGANIC MINERAL MANURES AND THEIR EFFICACY IN MAIZE(DEPARTMENT OF AGRICULTURAL MICROBIOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND, 2021) Solanki Jayvirsinh Pratapsinh; Dr. R. V. VyasThe nutrient pool and soil organisms are highly affected due to declining soil health. Reduction of fertilizer use efficiency is the reflection of injudicious use of agrochemicals for crop nutrition and protection, such activity lead to lower organic carbon content in soil. Restoration of soil health, lowering down chemical fertilizer requirement and enhancement of microbial activity was thought and attempted in present study using native plant growth promoting fungal isolates (PGPF). Fungal cultures were isolated from different AAU farms based on their abilities to solubilize P and K mineral forms. They were further screened for pathogenicity symptoms on seed germination and seedling growth parameters. Two non-pathogenic fungi were identified as isolate T-3, Aspergillus flavipes (NCBI Access No. MW88450) and T-17, Trichoderma aggressivum (NCBI Access No. MW88453).
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