Browsing by Author "Yadav, Asha"
Now showing 1 - 4 of 4
Results Per Page
Sort Options
ThesisItem Open Access Effect of Heat Stress on the Development and Gene Expression of In Vitro Produced Buffalo (Bubalus bubalis) Embryos.(NDRI, Karnal, 2012) Yadav, Asha; Chouhan, M.S.ThesisItem Open Access Genotypic and phenotypic analysis of elite inbred lines in pearl millet (Pennisetum glaucum (L.) R. Br.)(CCSHAU, 2016) Yadav, Asha; Dalal, M.SPhenotypic and genotypic diversity in pearl millet [Pennisetum glaucum (L.) R. Br.] was studied in a set of 40 inbred lines using 28 morphological traits (20 qualitative and 8 quantitative) and 16 SSR markers. Mean squares were found to be significant for all the characters studied except for the panicle diameter which indicated substantial amount of variation in the selected lines. Grain yield per plant (g), dry fodder yield per plant (g) and productive tillers (nos.) had high PCV, GCV, heritability and genetic advance as percent of mean. The correlation coefficients at genotypic level had higher magnitude than their corresponding correlation coefficients at phenotypic level, thereby, revealing strong inherent association of 1000 grain weight (g), dry fodder yield per plant (g), plant height (cm), panicle length (cm) and productive tillers (no.). Path coefficient analysis revealed that dry fodder yield per plant (g), productive tillers (nos.), panicle length (cm) and 1000 grain weight (g) had high direct effect towards grain yield per plant. Low values of Residual effect (0.88) indicated upto 92 percent contribution of independent characters included in the study. D2 analysis led to formation of six clusters. Maximum intra-cluster distance was observed for cluster 4 (3.105). Genetic analysis with 16 polymorphic SSR markers led to formation of 8 groups. Six out of 16 polymorphic markers showed more than 0.70 PIC values.Cluster 3 and 6 showed maximum inter cluster distance of (8.26), followed by cluster 4 and 6 (7.15) which indicated that elite inbred lines included in these clusters are genetically diverse and can be used as promising parents for further crop improvement programmes.. The confirmation of immense diversity among the selected elite inbreds may be of paramount importance for further crop improvement programme in pearl millet.ThesisItem Open Access Genotypic diversity and stability analysis in chickpea (Cicer arietinum L.)(CCSHAU, 2010) Yadav, Asha; Yadav, C.K.Fifty genotypes of chickpea were evaluated in three different environments for the nature of variability and stability for eleven traits viz., days to 50% flowering, days to maturity, plant height , number of branches per plant, number of pods per plant, number of seeds per pod, biological yield, seed yield per plant, harvest index (%), 100 seed weight and protein content ( %). Analysis of variance revealed significant differences among the genotypes for all the characters studied. The highest GCV and PCV were observed for number of branches per plant followed by 100 seed weight, number of pods per plant, seed yield per plant, biological yield per plant. The highest heritability value was registered for protein content (%), plant height, days to 50% flowering, seed yield per plant, biological yield per plant. Genetic advance as % of mean was highest for 100 seed weight in E1 and E2 followed by seed yield per plant for number of pods per plant in E3 followed by number of branches per plant, harvest index and seed yield per plant. In the present study high heritability estimates accompanied with high genetic advance were observed for 100 seed weight in all the three environments. These genotypes were grouped into eight clusters each in first two environments (E1, E2) and into nine clusters in environment three (E3). On overall basis genotypes HC-3, HK-3, HK06-169, HK06-151, H05-10, H06-07, H06-32 and H07-23 were observed quite divergent in all the environments and could be used in hybridization programme for obtaining superior recombinants. Pooled analysis indicated wide differences between environments and differential behaviour of genotypes in different environments. Stability analysis showed that a major portion of genotype × environment (G × E) interaction was accompanied by linear component for days to 50% flowering, days to maturity, number of branches per plant, number of pods per plant, number of seeds per pod, biological yield, seed yield per plant, 100 seed weight, whereas, non-linear portion predominantly contributed towards plant height, number of branches per plant, harvest index and protein content (%). Environment two (E2) was observed to be best for most of the yield attributing traits. Based on stability parameters genotypes H06-79, H04-31, HK05-151, HK06-162, HK06-170, HK06-171, HK-2, HK-3, H06-32 were found stable in better environmental conditions, genotype HC-3 for poor environment and genotypes H05-10, HK06-152, HK06-155 across the environments.ThesisItem Open Access In vitro mass multiplication and variability in stevia (Stevia rebaudiana bertoni)(CCSHAU, 2013) Yadav, Asha; Yadav, I.S.The present investigation was undertaken with the objectives like (i) to develop an efficient and reproducible mass multiplication protocol in stevia (ii) to evaluate the genetic variability among regenerated plants of Stevia at molecular level (iii) to compare various agronomic traits between tissue culture raised and conventionally grown plants of stevia. The shoot-tip and nodal segments were used as explants. Among the different sterilizing agents used, the maximum (100%) survival was recorded on when nodal explants were treated with HgCl2 (0.1%) for 3 minutes along with bavistin (0.2%) and streptocyclin (0.2%) for 45 minutes. The nodal and shoot-tip explants were best regenerated on MS medium+ BAP 2.0mg/l. Among the different media used, MS medium supplemented with 2.0 mg/l BAP showed the maximum shoot induction (98.0%) in 4.7 days and (74.0%) in 7.1 days using nodal and shoot tip explants, respectively.MS medium supplemented with BAP 2.0 mg/l was found to be most effective for establishment of both the explants. The best shoot multiplication medium was MS medium supplemented with BAP (0.3mg/l) + Kinetin (0.3 mg/l+0.1 mg/l NAA+ 15 mg/l PEG), on which an average MS basal medium supplemented with 0.3mg/l BAP + 0.3 mg/l Kin + 0.1 mg/l NAA+ 15 mg/l PEG was proved to be the best with 40.56 shoots/ explant developed from nodal and shoot-tip explants after 30 days on culture medium. Maximum rooting (90±7.75%) was observed on the half MS medium supplemented with 0.5 mg/l NAA with 21.2 roots per shoot in 7.4 days. The survival of in vitro raised plantlets was reported to be 100% in potting mixture containing sand + soil + FYM (1:1:1) and 100% in sand + soil + vermi compost (1:1:1) under green house conditions. For genetic fidelity/ variability, each primer generated a unique set of amplification products ranging in size from 100 to 1100 bps. All the bands in all the primers were same and no polymorphism was detected during the RAPD analysis of in vitro raised plants, which showed that all the plants raised through micropropagation were true to type or identical to the mother plant. Comparison of various agronomic traits between tissue culture raised and conventionally grown plants of stevia was carried out in the field. Analysis of variance indicated that in vitro raised plants were significantly superior for all agronomic traits over conventionally grown plants of stevia. The survival of tissue culture raised plants was observed to be 90% under field conditions, while 100% survival was reported in the green house.