Browsing by Author "Walia, Priyanka"
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ThesisItem Open Access Bioprospection of medicinal and aromatic plant resources of CSKHPKV premises(CSKHPKV, Palampur, 2010) Walia, Priyanka; Singh, AnitaThesisItem Open Access Linkage mapping of QTLs for grain iron content and evaluation of PAU201 x Palman 579 (iron rich) segregating population(s) in rice (Oryza sativa L.)(CCSHAU, 2015) Walia, Priyanka; Jain, Rajinder KumarLinkage mapping and marker assisted selection can greatly accelerate the efficacy of breeding programs to improve mineral (iron and zinc) content in rice grains. A F2 population developed from the cross between PAU201 (high yielding indica) and Palman 579 (iron rich indica) was used to map the quantitative trait loci (QTL) associated with grain mineral content; the population consisted of 50 plants displaying wide variation in grain mineral content. As many as 82/327 polymorphic microsatellite (SSR) markers were used for linkage mapping. A total of 187 alleles and 18 new recombinant alleles (different that those in parent rice varieties) were identified. NTSYS-pc based analysis (cluster tree analysis, 2D-PCA and 3D-PCA) showed dispersion of the F2 population between the two distinct parental genotypes. Composite interval mapping (CIM) analysis by WinQTL cartographer 2.5 revealed a total of nineteen QTL for mineral content in grains on chromosome 2 and 10. Eight QTL for iron content were mapped on chromosome 2 (qFE1, qFE2, qFE3, qFE4, qFE5, qFE6, qFE7 and qFE8 at map positions 34.8, 54.2, 63.5, 70.0, 90.1, 152.1, 160.0 and 182.6 cM respectively) and four on chromosome 10 (qFE9, qFE10 qFE11 and qFE12 at map positions 19.4, 37.4, 54.9 and 72.8 cM respectively). Seven QTL for zinc content were mapped on chromosome 2 (qZN1, qZN2, qZn3 and qZN4 at map positions 88.3, 93.6, 152.5 and 158.5 cM respectively) and chromosome 10 (qZN5, qZN6 and qZN7, at map positions 36.2, 45.5 and 53.8 respectively). A notable aspect of this study is that QTL qFE5, qFE6 and qFE7 (90.1, 152.1 and 158.5 cM) for iron content and qZN1, qZN3 and qZN 4 (88.3, 152.5 and 158.5 cM) for zinc content on chromosome 2 are in close proximity, respectively, with the same flanking markers. Similarly, on chromosome 10, qFE10 and qFE11 (37.3 and 54.9 cM) are in close proximity of qZN5and qZN7 (36.2 and 53.8 cM), respectively, thus indicating that these QTL for iron and zinc content may in fact be linked. PAU201/Palman 579 F 3 and F 4 populations were evaluated for various agronomic traits and mineral content which displayed large variation for plant height (55.5 - 158.0 cm), effective number of tillers per plant (1 - 31), panicle length (15.2 - 30.6 cm), grain yield per plant (1.6 - 66.6 g), 1000 grain weight (10.5 - 33.3 g) and grain length/breadth ratio (2.72 - 4.83), Fe content (4.7 - 508.8 µg/g) and Zn content (1.2 - 130.4 µg/g) . Notably, nine F3 plants and eleven F 4 plants had iron content more than iron-rich donor parent, Palman 579. Grain iron content showed significant positive correlation (r=0.829 and 0.335) with grain zinc content in both F 3 and F 4 populations indicating the feasibility of improving Fe and Zn levels simultaneously in rice grains. As many as 50 promising F4 plants with grains rich in iron, zinc or both iron and zinc were selected for the future breeding programs.ThesisItem Open Access Micropropagation studies in elite cultivars of gerbera (Gerbera jamesonii Bolus)(CCSHAU, 2009) Walia, Priyanka; Chowdhury, V.K.The present study was undertaken to develop an efficient protocol for micropropagation in Gerbera (Gerbera jamesonii Bolus) cultivars Doni and Danna Ellen. Capitulum, basal cutting and shoot-tip were used as explants in both the cultivars. Capitilum explants were surface sterilized with 0.4% bavistin for 30 min followed by treatment of 0.05% streptocyclin for 5 min and 0.1% (citric acid + ascorbic acid) treatment for 5 min which was followed by 0.1% -viii- HgCl2 treatment for 2.5 min which resulted in 80% sterilization of explants in both the cultivars. Shoot-tip explants were treated with 0.1% citric acid + 0.1% ascorbic acid for 10 min and then surface sterilized with 0.4% bavistin for 15 min, 0.05% streptocyclin for 5 min and then explants were washed with 70% ethanol for 30 sec followed by treatment with 0.1% HgCl2 for 2 min which resulted in 75% sterilization of explants in both the cultivars. Different sterilization protocols were tried for surface sterilization of basal cutting explants in both the cultivars. But none of the sterilization protocol was found to be effective to avoid contamination of basal cutting explants in both the cultivars. Maximum number of shoot buds (32) per capitulum explant was obtained on MS basal medium supplemented with 5.0 mg/l BAP and 0.1 mg/l IAA in cv. Doni, followed by cv. Danna Ellen with 28 shoot buds per explant on the same medium. Shoot bud induction was not observed in both the cultivars on the medium containing 1.0 mg/l BAP and 0.1 mg/l IAA. The shoot buds regenerated from capitulum explants in both the cultivars were transferred on various media combinations for shoot regeneration. Maximum number of shoots (9.0) was found to be regenerated in both the cultivars per shoot bud clump cultured on MS basal medium containing 1.0 mg/l TDZ and 0.1 mg/l IAA. Maximum number of shoots (on an average of 3.6 shoots) per shoot-tip explant was obtained in cv. Doni followed by cv. Danna Ellen (3.0 shoots/explant) on the MS basal medium supplemented with 5.0 mg/l BAP and 0.1 mg/l IAA. Shoot regeneration was not observed from basal cutting explants in both the cultivars as contamination was not controlled inspite of trying different sterilization protocols. In vitro flowering was observed in both the cultivars when capitulum was used as explant. Rooting (80%) was observed from aseptically regenerated shoots from shoot-tip explants on ½ MS basal medium containing 0.5 mg/l IAA in both the cultivars.