Browsing by Author "Viswanathan, Kaliyaperumal"
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ArticleItem Open Access Chlorhexidine-calcium phosphate nanoparticles — Polymer mixer based wound healing cream and their applications(2016) Viswanathan, Kaliyaperumal; Monisha, P; Srinivasan, M; TANUVASIn this work, we developed a wound healing cream composed of two different polymers, namely chitosan and gelatin with chlorhexidine along with calcium phosphate nanoparticles. The physicochemical properties of the prepared cream were investigated based on SEM, EDX, Raman, FTIR and the results indicated that the cream contained gelatin, chitosan, calcium phosphate nanoparticles and chlorhexidine. The maximum swelling ratio studies indicated that the ratio was around of 52 ± 2.2 at pH 7.4 and the value was increased in acidic and alkaline pH. The antimicrobial activity was tested against bacteria and the results indicated that, both chlorhexidine and the hybrid cream devoid of chlorhexidine exhibited antimicrobial activity but the chlorhexidine impregnated cream showed three fold higher antimicrobial activity than without chlorhexidine. In vivo wound healing promoting activities of hybrid cream containing 0.4 mg/L chlorhexidine were evaluated on surgically induced dermal wounds in mice. The results indicated that the cream with incorporated chlorhexidine significantly enhanced healing compared with the control samples. For the field validations, the veterinary clinical animals were treated with the cream and showed enhanced healing capacity. In conclusion, a simple and efficient method for design of a novel wound healing cream has been developed for veterinary applications.ArticleItem Open Access Development of antibiotic selection kit towards veterinary applications using glycine passivated magnetic particles(Biosensors and Bioelectronics, 2014) Viswanathan, Kaliyaperumal; Raj, G. Dhinakar; Vadivoo, V. Senthil; Kumanan, K.; Prabakaran, Rajamanickam; TANUVASArticleItem Open Access Formulation of Newcastle disease virus coupled calcium phosphate nanoparticles: An effective strategy for oculonasal delivery to chicken(ElSevier, 2014) Viswanathan, Kaliyaperumal; Gopinath, Vadivel Ponnuswamy; Raj, G. Dhinakar; TANUVASArticleItem Open Access In ovo delivery of Newcastle disease virus conjugated hybrid calcium phosphate nanoparticle and to study the cytokine profile induction(ElSevier, 2014) Viswanathan, Kaliyaperumal; Ratish, P; Gopinath, V.P.; Janice, R; Raj, G. Dhinakar; TANUVASArticleItem Open Access Ketoconazole-conjugated ZnO nanoparticles based semi-solid formulation and study their impacts on skin disease(2018) Viswanathan, Kaliyaperumal; Vaiyamalai, Rajasekar; Babu, Diviya Bharathi; TANUVASIn this study, the ketoconazole-conjugated zinc oxide (ZnO) nanoparticles were prepared in a single-step approach using dextrose as an intermediate compound. The physical parameters confirmed the drug conjugation with ZnO and their size was around 70–75 nm. The drug loading and in vivo drug release studies indicated that the –CHO group from the dextrose increase the drug loading up to 65% and their release kinetics were also studied. The anti-fungal studies indicated that the prepared nanoparticles exhibit strong anti-fungal activity and the minimum concentration needed is 10 mg/ml. The nanoparticles loaded semi-solid gel was prepared using carbopol, methylparaben, propyl paraben and propylene glycol. The in vitro penetration of the ketoconazole-conjugated nanoparticles was studied using the skin. The results indicated that the semi-solid gel preparations influenced the penetration and also favoured the accumulation into the skin membrane. The veterinary clinical studies indicated that the prepared gel is highly suitable for treatment of Malassezia.ArticleItem Open Access Rapid determination of hydrogen peroxide produced by Lactobacillus using enzyme coupled rhodamine isocyanide/calcium phosphate nanoparticles(ElSevier, 2014) Viswanathan, Kaliyaperumal; Vadivoo, V.S.; Raj, G. Dhinakar; TANUVASArticleItem Open Access Rapid determinationofhydrogenperoxideproducedby Lactobacillus using enzymecoupledrhodamineisocyanide/calcium phosphatenanoparticles(2014) Viswanathan, Kaliyaperumal; Vadivoo, VS; Dhinakar Raj, G; TANUVASA sensitive method for detecting hydrogen peroxide (H202) using rhodamine isocyanide incorporated calcium phosphate nanoparticles (Rho/CaP) was developed. The synthesized nanoparticles were characterized based on transmission electron microscopy (TEM), scanning electron microscopy (SEM). Fourier transformed infrared spectroscopy (FTIR). Raman spectroscopy, energy»dispersive X-ray spectro- scopy (EDX) and X-ray diffraction patterns (XRD). To study the application, the nanoparticles were functionalized with horse radish peroxidase (HRP) based on aminopropyl triethoxy silane (APTES) and used as tools to detect H202. The detection strategy was based on fluorescence quenching or colorimetric detection.ArticleItem Open Access Studies on antimicrobial and wound healing applications of gauze coated with CHX–Ag hybrid NPs(2020) Viswanathan, Kaliyaperumal; Babu, Diviya Bharathi; Karuppannan, Chitra, et al.,; TANUVASIn this study, chlorhexidine (CHX)–silver (Ag) hybrid nanoparticles (NPs) coated gauze was developed, and their bactericidal effect and in vivo wound healing capacities were tested. A new method was developed to synthesise the NPs, wherein Ag nitrate mixed with sodium (Na) metaphosphate and reduced using Na borohydride. Finally, CHX digluconate was added to form the hybrid NPs. To study the antibacterial efficacy of particles, the minimal inhibition concentration and biofilm degradation capacity against Gram-positive and Gram-negative bacteria was studied using Escherichia coli and Staphylococcus aureus. The results indicated that the NP inhibited biofilm formation and was bactericidal as well. The gauze was doped with NPs, and its wound healing property was evaluated using mice model. Results indicated that the wound healing process was fastened by using the NPs gauze doped with NPs without the administration of antibiotics.