Browsing by Author "Verma, O. P."
Now showing 1 - 10 of 10
Results Per Page
Sort Options
ThesisItem Open Access Alkaline Protease Production Using Submerged Fermentation from Different Species of Bacillus(Sam Higginbottom Institute of Agriculture, Technology & Sciences (SHIATS), 2016) Idbeaa, Insherah Omar Ali; Verma, O. P.Alkaline protease is the most important group of protease enzymes utilized commercially in various arenas of industries, such as food, detergent, leather, dairy, pharmaceutical, diagnostics, and waste management. Microorganisms of specially those belonging to Bacillus genera serve as a vast repository of diverse set industrially important enzymes and utilized for the large scale enzyme production using fermentation technology. Bacteria were isolated from soil samples from Lucknow in India using serial dilution method. Different isolates were then screened for their alkaline protease producing capability using skim milk hydrolysis test..Isolates showing positives result for the skim milk hydrolysis test were identified based on their morphology by staining and their biochemical properties. DNA was extracted from all the isolate using Phenol:chloroform method. Quantification of the isolated DNA was performed by UV-Vis double beam spectrophotometer. The high yield DNA samples were amplified using 16S rRNA primers and based on bioinformatic analysis thus identified five different Bacillus species (Bacillus megaterium, Bacillus pumilus, Bacillus subtilis ,Bacillus chonii and Bacillus cereus) were identified. Eight fermentation media were used for production of Alkaline protease for identifying the media in which yield is maximum for the Alkaline protease enzyme production.Media (MD8) produced highest amounts of alkaline protease (62.5 U/mL). The study also aimed at determining the most optimized conditions for Alkaline protease production and activity from five different species of Bacillus like pH, metal ions, nitrogen source, carbon source and NaCl. The most optimized media and culture conditions will help to enhance the protease production which could play major role in various detergent industries.ThesisItem Open Access ALKALINE PROTEASE PRODUCTION USING SUBMERGED FERMENTATION FROM DIFFERENT SPECIES OF BACILLUS(Department of Molecular and Cellular Engineering Jacob School of Biotechnology and Bioengineering Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad-211007, 2016) ALI IDBEAA, INSHERAH OMAR; Verma, O. P.Alkaline protease is the most important group of protease enzymes utilized commercially in various arenas of industries, such as food, detergent, leather, dairy, pharmaceutical, diagnostics, and waste management. Microorganisms of specially those belonging to Bacillus genera serve as a vast repository of diverse set industrially important enzymes and utilized for the large scale enzyme production using fermentation technology. Bacteria were isolated from soil samples from Lucknow in India using serial dilution method. Different isolates were then screened for their alkaline protease producing capability using skim milk hydrolysis test..Isolates showing positives result for the skim milk hydrolysis test were identified based on their morphology by staining and their biochemical properties. DNA was extracted from all the isolate using Phenol:chloroform method. Quantification of the isolated DNA was performed by UV-Vis double beam spectrophotometer. The high yield DNA samples were amplified using 16S rRNA primers and based on bioinformatic analysis thus identified five different Bacillus species (Bacillus megaterium, Bacillus pumilus, Bacillus subtilis ,Bacillus chonii and Bacillus cereus) were identified. Eight fermentation media were used for production of Alkaline protease for identifying the media in which yield is maximum for the Alkaline protease enzyme production.Media (MD8) produced highest amounts of alkaline protease (62.5 U/mL). The study also aimed at determining the most optimized conditions for Alkaline protease production and activity from five different species of Bacillus like pH, metal ions, nitrogen source, carbon source and NaCl. The most optimized media and culture conditions will help to enhance the protease production which could play major role in various detergent industries.ReportItem Open Access Annual Report 2012-2013(Directorate of Water Management (ICAR), Bhubaneswar, Odisha, 2013) Kumar, Ashwani; Roy Chowdhury, S; Kar, Gouranga; Ghosh, Souvik; Nayak, A. K.; Verma, O. P.Introduction, Research Achievements: Rainwater management, Canal water management, Groundwater management, Waterlogged area management, On-farm Research, Extension and Training, Training organised for Women Empowerment, AICRP's , List of Publications, Awards/ Honours/Recognitions, RAC/IRC/IMC/AICRP Meetings, List of Completed/Ongoing/New In-house projects, Human Resource Development, List of Sponsored /Collaborative/ Consultancy Projects, Events organized, ....ReportItem Open Access Annual Report 2013-2014(Directorate of Water Management (ICAR), Bhubaneswar, Odisha, 2014) Kumar, Ashwani; Roy Chowdhury, S; Kar, Gouranga; Thakur, A. K.; Brahmanand, P. S.; Nayak, Ashok K.; Verma, O. P.Research Achievements : Rainwater Management, Canal Water Management, Groundwater Management, Waterlogged area Management, On-farm Research and Technology Dissemination, Events/ Training organised for Women Empowerment, AICRPs, List of Publications, Awards/ Honours/ Recognitions, RAC/ IRC/ AICRP Meetings, List of Completed/ Ongoing/ New In-house Projects, Human Resource Development, List of Sponsored / Collaborative/ Consultancy Projects, Events Organized, Weather Summaary, Promotion/Transfer/ Retirement, Personnel, FinanceReportItem Open Access Annual Report 2014-2015(ICAR-Indian Institute of Water Management (ICAR), Bhubaneswar (Odisha), 2015) Ambast, S. K.; Roy Chowdhury, S; Thakur, A. K.; Mandal, K. G.; Panigrahi, P; Sinha, M. K.; Verma, O. P.Research achievements, Events/Trainings Organized for Women Empowerment, AICRP, List of publications, Awards/Honours/Recognitions, RAC/IRC/IMC/AICRPs Meeting, List of Sponsored/Collaborative/Consultancy Projects, Events Organixed, Joining/ Promotion/Transfer/Retirement, Personnel, FinanceInstitutional PublicationsItem Open Access ICAR - IIWM Annual Report 2015-16(ICAR-Indian Institute of Water Management (ICAR), Bhubaneswar (Odisha), 2016) Ambast, S. K.; Thakur, A. K.; Mandal, K.G.; Panigrahi, P; Verma, O. P.; Rautaray, S. K.; Panda, R. K.; Mishra, A; RoyChowdhury, S; Das, Mstrategies for efficient management of on-farm water resources for sustainable agricultural productivity, coordinate research for generating location-specific technologies for efficient use of water resources. centre for training in agricultural water managementInstitutional PublicationsItem Open Access ICAR-IIWM News(ICAR-Indian Institute of Water Management, Bhubaneswar, Odisha, 2017) Ambast, S. K.; Thakur, A. K.; Mandal, K. G.; Panigrahi, P; Verma, O. P.; Dubey, RachanaInstitutional PublicationsItem Open Access Krushijala parichalana - eka drustipata(iCAR -Indian Institute of Water Management, Bhubaneswar, 2016) Panda, Rabindra Kumar; Sethi, Ranu Rani; Panigrahi, Prabhukalyana; Verma, O. P.; Ambast, Sunil KumarInstitutional PublicationsItem Open Access Technology Brochure(Directorate of Water Management (ICAR), Bhubaneswar-751023,Odisha, 2014) Das, Madhumita; Das, Madhumita; Verma, O. P.; Kumar, AThesisItem Open Access Validation of Transcription Factors Responsible for Concerted Expression of IE genes of BHV-1(Sam Higginbottom Institute of Agriculture, Technology & Sciences (SHIATS), 2016) Pokhriyal, Mayank; Verma, O. P.Bovine herpesvirus-1 (BoHV-1) is an important member of the Herpesviridae family. The lytic cycle of BoHV-1 progress through three stages of viral transcription. The first stage, immediate-early (IE), does not require prior viral gene expression and is essential for the second, early (E), and third, late (L), stages of the cycle. Like other herpesviruses, BoHV-1 creates a lifelong latency in sensory neurons of trigeminal ganglia. Reactivation from latency leads to triggers of the transcription cascade. IE genes are important class of genes for the BoHV-1 replication. IE genes which are transcribed from two different immediate early transcription units; immediate early transcription unit I (IETU1) encoding BICP0 and BICP4 sharing common promoter and transcription unit II(IETU2) encoding BICP 22. Contrary to this we found that the transcripts of BICP0 and BICP4 are transcribed by two different TATA less promoters.The BICP0 and BICP4 also show different levels of expression. Realtime PCR revealed that expression level of BICP4 continuously declines after attaining a peak level at 1hr while BICP0 show biphasic expression supporting the earlier observation that it is expressed from two different promoters. These promoters of immediate early genes are well characterized but still the transcription factors involved in the concerted expression of immediate early genes of BoHV-1 are unknown. Evidence is provided for the existence of three other immediate early genes i.e. UL21, UL33 and UL 34 in BoHV-1. These genes are expressed in the presence of cycloheximide and show a similar expression profile as BICP0, BICP22 and BICP4. The promoters of these genes have TATA box at optimal site, lack OCT-1 factor but show similar spatial placement of C/ EBPα, DPE and Inr elements. The TATA box present in optimal position make the basal promoter of UL21, UL33 and UL34 stronger than the promoters of BICP0, BICP4 and BICP22. Also role of OCT-1, is not essential in the coordinated expression of IE genes in BoHV-1 infection as it is for HSV-1.