Browsing by Author "TARANNUM"
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ThesisItem Open Access IN VITRO AND IN VIVO MUTAGENESIS IN CROSSANDRA (Crossandra infundibuliformis L. Nees)(UNIVERSITY OF HORTICULTURAL SCIENCES, BAGALKOT, 2015-05) TARANNUM; RAO, MANJUNATHA T.; B., FAKRUDDIN; M.V., DHANANJAYA; SAHIJRAM, LEELA; S., SRIRAM; S., JAGANATH; R., VENUGOPALANCrossandra, a popular flower in demand in southern India and foot rot is a limiting factor in its commercial cultivation. Five varieties were evaluated for resistance against Phytophthora nicotianae. ‘Arka Ambara’ and ‘Arka Shreeya’ were found to be resistant. Highest infection was recorded in ‘Local’ variety. For standardizing protocols for micropropagation and in vitro mutagenesis, ‘Arka Kanaka’ and ‘Arka Shravya’ were used. Highest number of multiple shoots was obtained on MS medium with Kn 0.5 mg l-1+BAP 0.5 mg l-1 or Kn 0.25 mg l-1+BAP 0.25 mg l-1 subcultured onto BAP 0.25 mg l-1 in ‘Arka Shravya’. In ‘Arka Kanaka,’ multiple shoots were maximum in Kn 0.25 mg l-1 subcultured onto BAP 0.25 mg l-1. Per cent rooting was low on MS medium, whereas WPM supplemented with cytokinin/auxin induced 100 % rooting on Kn 0.25 mg l-1+IBA 3 mg l-1. EMS mutagen in vitro gave shorter axillary shoots and lower rooting rate at increasing concentrations and duration of treatment. LD50 in ‘Arka Shravya’ was obtained on 1 % EMS for 45 min. and in ‘Arka Kanaka’ at 0.25 % EMS for 60 min. In vivo mutagenesis in rooted cuttings with 60Co gamma irradiation showed lower growth in all the varieties irradiated with 30 Gy, 40 Gy or 50 Gy. Leaf abnormality, delayed flowering, severe reduction in plant height, plant spread, leaf length and leaf width was observed at 40 Gy and 50 Gy in ‘Arka Ambara’, ‘Arka Shreeya’ and ‘Arka Shravya’ at 45 days after irradiation. Molecular profiling with RAPD analysis revealed that 24 polymorphic primers in ‘Arka Ambara’, 14 in ‘Arka Shreeya’ and 19 in ‘Arka Shravya’ indicating variation in DNA of control and irradiated plants in all varieties. The resistant varieties can be recommended for commercial cultivation. LD50 value will be useful for obtaining novel types. These studies can help to develop resistant/novel varieties using in vitro/in vivo techniques.ThesisItem Open Access PERFORMANCE OF CARNATION (Dianthus caryophyllus L.) GENOTYPES UNDER PROTECTED CULTIVATION(University of Horticultural Sciences, Bagalkot (COLLEGE OF HORTICULTURE, MUDIGERE ), 2012-07) TARANNUM; B., HEMLA NAIK; V., SRINIVASA; G., JANARDHAN; V.B., SANATH KUMAR; Y.S., RAMESHA; S.Y., CHANDRASHEKARThe Divine flower Carnation in the modern times has become one of the most important and highly remunerative flower crops grown under polyhouse, occupies an esteem position among top ten flower crops in the International trade mainly for its cut flowers. It is also used for bedding, pots, rock gardens, window boxes and edging. There is always demand for novel types with high yielding genotypes. Hence, the present study was conducted to identify the suitable Carnation genotypes under naturally ventilated polyhouse in hill zone during 2011-12, with respect to yield and quality cut flowers and also to find out the best chemical preservative for enhancing the vase life in the Department of Floriculture and Landscape Architecture, College of Horticulture, Mudigere. Among the eight genotypes studied there were wide and highly significant variations observed in performance with respect to growth, floral, cut flower yield and quality parameters. The cv. Soto produced maximum number of quality cut flowers and was found to be significantly superior over others. Dona and White Dona were next superior types. Soto produced the longest stalk length, bigger sized flowers with maximum number of petals whereas; stalk girth was more in White Dona followed by Soto and Dona. The genotype Soto, Golem and White Dona were early in flowering. Most of the morphological parameters viz., plant height, plant spread, number of branches, internodes and leaves; leaf length and width, leaf area, dry matter production, chlorophyll content etc., were found superior in cv. Soto followed by White Dona, Dona and Harish. The genotype ‘Soto’ realized maximum net return and B:C ratio ( 3,50,483; 2.50) in 560m2 area followed by Dona ( 2,80,343; 2.00, respectively) and White Dona ( 2,59,343;1.85) compared to other genotypes studied. In the vase life study, among the chemical preservatives (viz., citric acid, cobalt chloride and 8-HQS) tried, citric acid @ 200ppm recorded maximum vase life of Carnation (12 days) followed by citric acid @ 150 ppm (11 days ).