Browsing by Author "Sureshkumar, V"
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ArticleItem Open Access Evaluation of stability of enrofloxacin and chicken tissues exposed to various cooking processes ciprofloxacin antibiotic residues in broiler(2022) Sureshkumar, V; Sarathchandra, G; TANUVASThe effect of temperature on stability of drug residues in foods is imperative from consumer safety point of view. Hence, the effect of various cooking processes like boiling, roasting, microwaving and grilling on enrofloxacin and its metabolite ciprofloxacin residues in broiler chicken tissues was evaluated. Enrofloxacin and ciprofloxacin residues in control, raw and cooked tissues were analysed by a validated High performance thin layer chromatography-Fluorescent densitometry method. All the cooking processes resulted in significant reduction (p<0.01) in total enrofloxacin residues when compared to that of raw tissues. The highest decrease in residue levels was observed in boiled tissues; however the lost residues were detected in boiling fluid used for cooking. Among other cooking methods, roasting showed better reduction in residues followed by grilling and microwaving. In spite of that, the residue levels remained high in all the cooked tissues exceeding the maximum residue limits prescribed by European Union and Japan. The present study suggests that various cooking processes cannot completely deplete the enrofloxacin residues but it can only decrease their amounts.ArticleItem Open Access Evaluation of stability of enrofloxacin and chicken tissues exposed to various cooking processes ciprofloxacin antibiotic residues in broiler(2022) Sureshkumar, V; Sarathchandra, G; TANUVASThe effect of temperature on stability of drug residues in foods is imperative from consumer safety point of view. Hence, the effect of various cooking processes like boiling, roasting, microwaving and grilling on enrofloxacin and its metabolite ciprofloxacin residues in broiler chicken tissues was evaluated. Enrofloxacin and ciprofloxacin residues in control, raw and cooked tissues were analysed by a validated High performance thin layer chromatography-Fluorescent densitometry method. All the cooking processes resulted in significant reduction (p<0.01) in total enrofloxacin residues when compared to that of raw tissues. The highest decrease in residue levels was observed in boiled tissues; however the lost residues were detected in boiling fluid used for cooking. Among other cooking methods, roasting showed better reduction in residues followed by grilling and microwaving. In spite of that, the residue levels remained high in all the cooked tissues exceeding the maximum residue limits prescribed by European Union and Japan. The present study suggests that various cooking processes cannot completely deplete the enrofloxacin residues but it can only decrease their amounts.ArticleItem Open Access Excretion of enrofloxacin residues in poultry droppings after pulse water medication in broiler chicken – an environmental concern(2020) Sureshkumar, V; Sarathchandra, Ghadevaru; TANUVASThe presence of antibiotics in manures can represent an environmental and human health concern. Residues of antibiotics excreted into animal manures enter the environment either by spreading of livestock wastes onto agricultural fields as fertilizer or in the form of sludge after manure collection and storage. Livestock manure is a major source of veterinary antibiotics residues in agricultural fields as well as in the overall environment; hence analysis of these antibiotics is obviously important. Twelve one-day old broiler chicks were randomly divided into control (6 nos) and treatment group (6 nos). Treatment group received enrofloxacin @ 10 mg/Kg body weight, through drinking water for five consecutive days from 43rd to 47th day of age, whereas control group received non-medicated water. Dropping samples of control and treatment group were collected at different time points during the withdrawal period at 48 hours interval on day 1, 3, 5, 7 and 9 post treatments. Enrofloxacin and ciprofloxacin residues in poultry dropping samples were analysed by a validated High Performance Thin Layer Chromatography-Fluorescent Densitometry method. Enrofloxacin residues could be detected in droppings even up to 7th day after treatment. Owing to the presence of antibiotics in droppings even after cessation of the administration of enrofloxacin, it warrants environmental concern. These antibiotic residues in animal faeces may potentially bring ecological risks. The present study stresses the need for stringent regulation for the use of antimicrobial drugs in the poultry industry.ArticleItem Open Access Phytochemical Screening and Thin Layer Chromatography Profiling of Various Extracts of Achyranthes aspera and Cissus quadrangularis(2021) Sureshkumar, V; TANUVASAchyranthes aspera and Cissus quadrangularis are important medicinal plants possessing wide biological activity. The natural products obtained from plants provides an unique opportunity for the development of new drugs but due to their complex nature there is a need to isolate and purify the bioactive compounds from plant extracts by separation techniques. Our earlier findings revealed that methanol extracts of A. aspera and C. quadrangularis and aqueous extract of C. quadrangularis exhibited antibacterial activity. This prompted to take up further research to reveal these plants’ potential valuable phytochemicals and therefore an attempt has been made to separate the phytochemical constituents of methanol and aqueous extracts of A. aspera and C. quadrangularis by thin layer chromatography. Phytochemical screening of both plant extracts revealed more concentrated phytochemicals in methanol extracts than in aqueous extracts. The TLC profiling showed that methanol extract of A. aspera and C. quadrangularis are rich in flavonoids and phenolic compounds. Flavonoids were separated with n-butanol:ethyl acetate:water (1:2:3) solvent system at Rf value of 0.66 and was confirmed by post-development derivatization with 3% boric acid and 10% oxalic acid spray. After derivatization, the flavonoid spot could be further visualized under UV light at 254nm. Phenolic compounds were separated with methanol:water (2:1) solvent system at Rf value of 0.88 and confirmed with 10% ferric chloride spray. From the present study, suitable mobile phase for separation of flavonoids and phenolic compound fraction from methanol and aqueous extracts of both plants and TLC profiling have been developed.Book chapterItem Open Access மரபுசார் மூலிகை மருத்துவத்தின் மூலம் செம்மறி ஆடுகளில் குடற்புழு நீக்கம்(2017) Ramakrishnan, V; Punniyamurthy, N; Jagadeeswaran, A; Sureshkumar, V; Anna, T; Meenakshi Sundaram, A; TANUVASBook chapterItem Open Access