Browsing by Author "Suresh, Ashitha"
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ArticleItem Open Access Simulated physiological oocyte maturation (SPOM) improves developmental competence of in vitro produced goat embryos(2021) Suresh, Ashitha; Shukla, MK; Kumar, Dharmendra, et al.,; TANUVASThe effect of simulated physiological oocyte maturation on the developmental competence, reactive oxygen species production and apoptosis rate of in vitro produced goat embryos were studied in the present experiment. Oocytes and spermatozoa were recovered from ovaries and epididymis, respectively, procured from a local small animal abattoir. The oocytes aspirated from the ovaries were allocated into two groups, control (subjected to routine in vitro maturation, fertilization and culture) and simulated physiological oocyte maturation (SPOM) group (subjected to prematuration, followed by routine in vitro maturation, fertilization and culture). The SPOM group showed a significantly (p < 0.05) higher maturation and blastocyst rates (90.60 ± 0.46% and 29.09 ± 2.59%, respectively) as compared to the control group (85.29 ± 0.98% and 24.09 ± 1.08%). The intensity of reactive oxygen species of the embryos in the control group (14.98 ± 0.83 pixels/embryo) was significantly (p < 0.05) higher than the SPOM group (9.60 ± 0.76 pixels/embryo). The apoptosis rate was also significantly (p < 0.05) higher in the embryos of the control group (9.18 ± 1.07%) as compared to the SPOM group (5.71 ± 0.90%). In conclusion, the simulated physiological oocyte maturation system significantly increases the developmental competence of the oocytes and decreases the intensity of reactive oxygen species and embryonic apoptosis in abattoir derived goat embryos.ArticleItem Open Access Surgical Management of Dystocia Due to Unilateral Uterine Torsion in a Domestic Shorthair Cat(2021) Ali, M; Suresh, Ashitha; Sarath, T, et al.,; TANUVASA 2-year-old pluriparous domestic shorthair queen was brought to the Madras Veterinary College Teaching hospital with a history of full-term pregnancy, straining for the past 24 hours and brown vaginal discharge. Radiography of the abdomen showed presence of 3 fetal skeletons, and ultrasonography revealed no fetal heartbeat. An emergency caesarean section was performed, and a 720° torsion of the right uterine horn was revealed. Three dead kittens were delivered, 2 from the right horn and 1 from the left horn. An ovariohysterectomy was also performed.ArticleItem Open Access Surgical Management of Dystocia Due to Unilateral Uterine Torsion in a Domestic Shorthair Cat(2021) Ali, M; Suresh, Ashitha; Sarath, T, et al.,; TANUVASA 2-year-old pluriparous domestic shorthair queen was brought to the Madras Veterinary College Teaching hospital with a history of full-term pregnancy, straining for the past 24 hours and brown vaginal discharge. Radiography of the abdomen showed presence of 3 fetal skeletons, and ultrasonography revealed no fetal heartbeat. An emergency caesarean section was performed, and a 720° torsion of the right uterine horn was revealed. Three dead kittens were delivered, 2 from the right horn and 1 from the left horn. An ovariohysterectomy was also performed.