Browsing by Author "Sumit Karwa"
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ThesisItem Open Access Osteopontin Gene Polymorphism and its Association with Milk Traits in Dangi Cattle(MAFSU, NAGPUR, 2023-04-03) Sumit KarwaThe current research was carried out at the Genetic Investigation Laboratory, Department of Animal Genetics and Breeding and the Department of Livestock Product Technology, Mumbai Veterinary College, Mumbai. The main goal of the current research was to pinpoint the polymorphism of the bovine Osteopontin gene’s intron-4 and its relationship to various milk characteristics, including milk fat percentage, protein percentage, SNF, and lactose content. For the current study, a total of 30 lactating Dangi cows from the Cattle Breeding Farm and Dangi Cow Research Station, Igatpuri, Dist. Nashik, were chosen. Every fortnightly, milk samples from each cow were collected and processed to estimate milk traits, and simultaneously blood samples were taken to extract genomic DNA. Blood samples were used to extract the genomic DNA, which were then quantified and evaluated for purity. With an O.D. ratio of 1.8 to 2, all DNA samples were determined to be of good quality. The bovine Osteopontin gene’s intron-4 was amplified using PCR and an appropriate primer. At a touch-down PCR annealing temperature range 63ºC to 50ºC (-2ºC/cycle), the amplified PCR product of size 290 bp was produced. Two band patterns were generated in Dangi cows by restriction digestion with BsrI enzyme at 65 ºC for 4.5 hours. The genotypes of the cows were determined using the BsrI-RFLP analysis pattern as TT (290 bp) and CT (290, 200, 90 bp). It was found that the studied group of Dangi cattle lacked the CC genotype. In 30 Dangi cows, the genotypic frequencies of TT (29 cows) and CT (01 cow) were 0.97 and 0.03, respectively, and allelic frequencies for T and C were 0.98 and 0.02, respectively. It confirms the polymorphic nature of OPN gene and proves the predominance of ‘T’ allele in Dangi cattle population under study. The average estimated values for milk fat, milk protein, SNF and lactose percentage were 3.85±0.18, 3.15±0.03, 8.55±0.10 and 4.70±0.05, respectively in Dangi cows. In the present study, the association between the OPN gene polymorphism and milk traits could not be established due to insufficient data. Therefore, it is advised that the said study be continued and expanded to include a larger population.