Browsing by Author "Sukumar, Bharathy"
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ArticleItem Open Access Goat milk as a non-invasive sample for confirmation of Mycobacterium avium subspecies paratuberculosis by IS900 PCR(2014) Sukumar, Bharathy; Gunaseelan, Lakshmanasami; Porteen, Kannan; Prabu, Karuppanasamy; TANUVASMycobacterium avium subsp. paratuberculosis (MAP) causes Johne's disease (JD) in cattle, sheep, goats and other ruminants, and Crohn’s disease in humans. MAPs are shed to external environment through feces and milk. The present study was aimed to evaluate the utility of milk as a non-invasive sample in stage II MAP infections in goats using IS900 polymerase chain reaction (PCR) and sequencing analysis. A total of 32 milk samples from lactating does were collected. Within these 32 milk samples, 15 were collected from pre-confirmed JD positive goats. By IS900 PCR, all the 15 (100%) known JD positive goat milk samples revealed the presence of MAP. However, no unknown goat was identified as MAP positive. The results of this study established the usefulness of milk as a non-invasive sample in screening and confirmation of stage II MAP infection in goats.ArticleItem Open Access Milk Ring Test for spot identification of Brucella abortus infection in single cow herds(2014) Mohamand, Najibullah; Gunaseelan, Lakshmanasami; Sukumar, Bharathy; Porteen, Kannan; TANUVASIn this study, milk samples were collected from 109 dairy cows to detect antibodies against Brucella (B.) using Milk Ring Test (MRT). Overall, 18.35% (n=20/109) of the milk samples were positive by MRT. The cows were divided into three groups based on lactation number viz., 1st, 2nd to 4th and ≥5th lactations; the prevalence of brucellosis in the groups were found to be 0.92% (n=1/109), 15.60% (n=17/109) and 1.83% (n=2/109), respectively. Considering simplicity and cost effectiveness, the MRT can be used for the preliminary screening of B. abortus infection especially in single cow herds.