Browsing by Author "Sinha, Bhav Kumar"
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ThesisItem Open Access Bio-prospecting of selenium fortification in Mustard (Brassica juncea L.)(Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, 2022-11) Shah, Suhail Ashraf; Sinha, Bhav KumarThe present study entitled, “Bio-Prospecting of Selenium Fortification in Mustard (Brassica juncea L.)” was carried out to study the effect of Selenium on germination and morpho-physiological growth of mustard(Brassica juncea L.)andthe effect of Se for development of resistance against drought grown under pot conditions and effect of Se on morphological, biochemical and yield parameters. The experiment was carried at Division of Plant Physiology, Faculty of Basic Sciences SKUAST Jammu. Pusa bold variety of Brassica juncea L. was taken under study. The experiment was laid under completely Randomized Block Design with four replications and fifteen treatments. The foliar application of Se was given in vegetative and reproductive stage. From the study it was demonstrated that the effect of different levels of Se on plant height, number of leaves/plant and number of branches/plant significantly higher in 4 ppm Se with 7 days WOW(178.6, 77.65, 12.37, respectively). Similarly fresh weight leaves, stem, root and silique(5.09, 38.40, 7.88, 36.92 g) and dry weight of leaves, stem, root and silique (2.94, 29.14, 5.80 and 31.94 g), respectively was observed in 4 ppm Se with 7 days WOW. However in case of biochemical parameters like chlorophyll content, chl a, chl b, total chlorophyll and total carotenoid, obtained maximum value (47.25, 1.31, 0.73, 1.94, 0.46), respectively in treatment 30 ppm Se with 7days of WOW. Expect that of pollen viability (63.45%), total soluble sugar (3.93 mg/g) and total starch (180.26 mg/g) which was obtained significantly highest in T15, T2 and T6,respectively. But In case of proline in 60 DAS (2.22 mg/g FW) and 90 DAS (1.81 mg/g FW) was obtained highest in T13. However in case of relative stress injury the highest was obtained in T8. In case of growth parameters like RWLC (85.00 %) the highest value was obtained in control. Whereas, CGR (2.49 g/m/day), RGR (0.190 g/g/day), SLA (1351 cm2/g), SLW (0.0023 g/cm2) and LAD (87.44 cm2/day) maximum value was obtained in 4ppm Se with holding of water for 7 days. For yield and quality parameters like Number of silique/plant(115.37), seed yield (42.60 g) and test weight (5.70 g) were found to be maximum in 4 ppm Se with 7 days WOW. Fatty acid analysis was found to be significantly highest in 30 ppm Se with 7 days WOW. However the Se content in seed (0.63 mg/kg) and Se content in soil (1.63 mg/kg) were found to be significantly highest in 30 ppm Se with 7 days WOW. Thus, it can be concluded that treatment T2 (4 ppm Se with 7 days WOW) resulted in significantly higher growth, yield attributes and yield. Besides this, Se also enhanced the chlorophyll, proline content significantly. Therefore, treatment T2 (4 ppm Se with 7 days WOW), can be recommended as an effective approach of Se fortification for enhancing growth, and yield and quality of crop.ThesisItem Open Access Impact of Bioregulators on Rooting of Stem Cutting in Passion Fruit (Passiflora edulis flavicarpa Deg.)(Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu, 2023-11-14) Kalsi, Jyotsana; Sinha, Bhav KumarDetailed systemic studies were conducted on impact of bioregulators on rooting of stem cutting in Passion fruit (PassifloraedulisflavicarpaDeg.) in the Division of Plant Physiology, Faculty of Basic Sciences, SKUAST- Jammu. Yellow passion fruits were taken as experimental material. The stem cuttings of about 15-20 cm length with 3-4 nodes were collected from mother plant of passion fruit and then treated with various concentration of IBAand PBZ. Cuttings are then, planted in polybags containing potting mixture (soil: sand: vermicompost: cocopeat) in equal proportion. Sprouting of cuttings was observed after 30-35 days after planting (DAP). The experiment was laid out in Completely Randomized Design (CRD) with seven different treatments viz., T1 (control), T2 (IBA300 ppm), T3 (IBA 500 ppm), T4 (IBA700 ppm), T5 (PBZ 20 ppm), T6 (PBZ 40 ppm) and T7 (PBZ60 ppm). The results showed that, number of sprouted cuttings and sprouting percentage was significantly increased in 60 ppm PBZ treated cuttings(5.67 and 56.7 %) as compared to control (3.00 and 30.0 %). Length of longest sprout 90 DAP and shoot diameter was recorded highest in 700 ppm IBA (14.3) and60 ppm PBZ (1.83) and lowest in control (4.47 and 0.85 cm) respectively. The significant increase was observed in number of shoots, leaves per cutting and leaf area when cuttings were subjected to 60 ppm PBZ (1.77, 7.33 and 17.4 cm2) in comparison to the control (0.50, 2.33 and 14.2 cm2). Length and diameter of roots and survival percentage was significantly increased in PBZ treated cuttings @ 60 ppm (13.6 cm, 1.79 cm and 43.3%) in comparison to control (4.50 cm, 0.57cm and 26.7 %). As far as physiological parameters i.e. fresh and dry weight of leaves, stemsand roots and partitioning coefficient of leaves and stems was significantly increased in cuttings treated with60 ppm PBZ (28.4, 2.48, 5.99, 0.57g and 8.73 %) as compared to control (19.6, 1.07, 2.15, 0.06 g and 2.56 %) respectively while significant decrease in partitioning coefficient of roots was observed in 60 ppm PBZ(91.3 %) as compared to control (97.5 %). Similarly, biochemical parameters i.e. chlorophyll content, carotenoid and total soluble sugar (TSS)was also increased significantly in 60 ppm PBZ treated cuttings(5.27, 1.06and3.07 mg/g F.W) as compared to control (2.98, 0.46 and 2.57 mg/g F.W) respectively. Benefit: Cost ratio (B:C) was reported maximum in PBZ @ 60 ppm (1: 2.06) as compared to control (1: 1.28).Our results clearly indicated that, 60 ppm PBZ was most effective treatment in rooting of stem cutting in passion fruit and enhance morphological, physiological and biochemical parameters.ThesisItem Open Access Influence of Bioregulators on Seed Germination of Weedy Rice in Jammu Region(Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu, 2023-11-29) Malpotra, Sofia; Sinha, Bhav KumarPresent study entitled,“Influence of bioregulators on seed germination of weedy rice in Jammu region”was carried out in the Division of Plant Physiology, Faculty of Basic Sciences, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu. Survey has been done in the farmer’s field of Jammu region and experimental field of SKUAST-Jammu. Three different genotypes of weedy rice were identified. Seeds of weedy rice were used for observation of germination percentage in the Laboratory of Division of Plant Physiology, SKUAST–J Chatha. After cleaning seeds of weedy rice grown in petri-dishes using germination paper and treated with various concentration of IAA and GA. The experiment was laid out in Completely Randomized Design (CRD) with eleven different treatments viz., T1 (IAA @ 0.1ppm), T2 (IAA @ 0.5ppm), T3 (IAA @ 1.0ppm), T4 (IAA @ 2.0ppm), T5 (IAA @ 2.5ppm), T6 (GA @ 05ppm), T7 (GA @ 10ppm), T8 (GA @ 15ppm), T9 (GA @ 20ppm), T10 (GA @ 25ppm) and T11(Control). Plant height (cm), number of leaves, productive and unproductive tillers was noticed significantly higher in Awnless brown husk (150.66 cm, 56.33, 19.0, 17.0 and 2.33) followed by Awns brown husk (146.33 cm, 52.0, 16.33, 14.66 and 2.0) in comparison to Awnless dark brown husk (129.0 cm, 42.0, 14.33, 13.0 and 2.0). Number of grains/panicle, grain yield/ plant, leaf fresh, leaf dry and test weight (g) was also increased significantly in Awnless brown husk (225.0, 6.41 g, 47.29 g, 11.53 g and 26.20 g) followed by Awns brown husk (206.0, 5.49 g, 46.66 g, 10.80 g and 22.90 g). Chlorophyll (SPAD value), chlorophyll ‘a’, chlorophyll ‘b’ and total chlorophyllwas observed significantly higher in Awnless brown husk (67.63, 3.47 mg/g F.W, 0.62 mg/g F.Wand 4.09 mg/g F.W) in comparison to Awnless dark brown husk (52.26, 2.42 mg/g F.W, 0.50 mg/g F.W, and 2.92 mg/g F.W). Where as in experiment 2, as far as morpho-physiological parameters days required for seed germination of 1%, 50% and 100% was noticed significantly lower when plants treated with GA@ 25 ppm (2.66 , 5.33 and 10.77) days in comparison to control (4.77, 8.0 and 13.0) days. Seedling length (cm) was maximum in plants treated with GA @ 25 ppm (9.81 cm) as compared to control (7.44 cm). Therefore, it has been concluded that GA @25 ppm is responsible for early germination of weedy rice in the field and can manage weedy rice population in the farmer’s field by cultural activity and is responsible for increasing the production and productivity of rice crop.ThesisItem Open Access Physiological Studies On Photosynthesis And 14C Translocation Under Water Logging And High Temperature Stress In Indian Mustard (Brassica Juncea (L.) Czern And Coss.)(Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2004) Sinha, Bhav Kumar; Chhabra, M.L.ThesisItem Open Access Study on Growth Retardants for Ameliorating the Effect of Drought Stress in Mustard (Brassica juncea L)(Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, 2023-05) Banoo, Muneeba; Sinha, Bhav KumarDetailed systemic studies were conducted on the study of growth retardants for ameliorating the effect of drought stress in mustard (Brassica juncea L.).RH-30, RSPR-01 and Pusa bold varieties were taken as an experimental material. The foliar spray of growth regulators Paclobutrazol (PBZ) and Chlormequat chloride (CCC) were given at vegetative stage. PBZ and CCCwere applied at different concentration (100 ppm, 150 ppm, 200 ppm and 250 ppm) and (500 ppm, 800 ppm, 1100 ppm and 1400 ppm) respectively. PBZ and CCCwere used as biochemical hormone to reduce the amount of water requirements and increase crop water use efficiency and to study their effect on lodging and pod shattering inmustard crop. The results revealed that PBZ and CCC application induced themorphological, physiological and biochemical responses that minimized the effect of drought stress in Brassica juncea genotypes grown under rainfed condition. In relation to morphological responses, a significant reduction in plant height was recorded inCCC treated plants @ 1100 ppm (77.4 cm, 70.6 and 66.4 cm) followed by PBZ @ 200 ppm (80.4, 75.7 and 70.4 cm) in comparison to control (134.6 cm,125.4 cm and 112.8 cm) in varieties RH-30, RSPR-01 and Pusa bold respectively. Highestrelativewater content was recorded in plants treated with CCC @ 1100 ppm (92.31, 84.44 and 79.33 %) followed by PBZ @ 200 ppm (89.38, 79.83 and 77.12 %) and lowest was observed in control (74.29, 65.42 and 60.23 %) in varieties RH-30, RSPR-01 and Pusa bold respectively.Maximum relative stress injury was observed in control plants in comparison to treated plants. Maximum photosynthetic rate (μmol CO2 /m2 /s) was observed in CCC treated plants @ 1100 ppm and PBZ @ 200 ppm in comparison to control. Highest yield/plant (g) was observed in CCC treated plants @ 1100 ppm (23.2 g) followed by PBZ @ 200 ppm (20.9) in comparison to control (9.2 g).A marked increase in cellulose and lignin content (g) were recorded in CCC treated plants @ 1100 ppm and PBZ @ 200 ppm in comparison to control. Results of all morphological, physiological and biochemical responses indicated that CCC @1100 ppm and PBZ @200 ppm causing reduced height and increased stem diameter thus leading to smaller plants with relatively better performances under water limiting condition. It also plays a significant role in tolerance tolodging and pod shattering in mustard crops.