Browsing by Author "Shilpa, P."
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ArticleItem Open Access Assessment of cellular and mucosal immune responses in chicks to Newcastle disease oral pellet vaccine (D58 strain) using qPCR(2014-10) Shilpa, P.; Kirubaharan, J. John; Chandran, N. Daniel Joy; Gnanapriya, N.; TANUVASTo assess the cell mediated and mucosal immune responses in chicks to Newcastle disease vaccine, expression levels of certain genes encoding cytokines and chemokines were quantified by q-PCR. The utility of cytokine and chemokine gene expression profile in estimating the cell mediated and humoral immune response has been established. The cell mediated immune response was assessed by quantifying the IFN-c gene expression in splenocytes and compared with colorimetric blastogenesis assay. The mucosal immune response was assessed by quantifying the expression of IL-8, IL1-b, MIP1-b, K60 and K203 in the intestinal cells and compared with IgA ELISA. On 14th day post vaccination, the expression of IFN-c was upregulated by 12-folds in the Group I, which have received oral pellet vaccine and fourfolds in the Group II where birds have received live thermostable vaccine as occulonasal instillation. 3 and 7 days after receiving booster, the same cytokine gene was upregulated by 12-folds and 27-folds respectively in the Group III, where birds have received live thermostable ND vaccine as priming vaccine and oral pellet vaccine as booster. On 21st day post vaccination the expression of IL-8 was upregulated by 42.8-folds in Group I and 3.3-folds in the Group II. The expression of IL-1b was upregulated by eightfolds on 3rd day post vaccination and 23-folds on 21st day post vaccination in Group I. The expression of macrophage inflammatory protein-1b (MIP-1b) was upregulated by 16-folds in Group I and 70-folds in Group II on 14th day post vaccination. No significant change in expression of chemokine genes K60 and K203 in vaccinated birds. The results were comparable with the results of conventional tests and proved the utility of qPCR in estimating the cellular and mucosal immune responses.ArticleItem Open Access Assessment of cellular and mucosal immune responses in chicks to Newcastle disease oral pellet vaccine (D58 strain) using qPCR(2014-10) Shilpa, P.; Kirubaharan, J. John; Chandran, N. Daniel Joy; Gnanapriya, N.; TANUVASTo assess the cell mediated and mucosal immune responses in chicks to Newcastle disease vaccine, expression levels of certain genes encoding cytokines and chemokines were quantified by q-PCR. The utility of cytokine and chemokine gene expression profile in estimating the cell mediated and humoral immune response has been established.ArticleItem Open Access CHARACTERISATION OF INFECTIOUS BRONCHITIS VIRUS ISOLATE FROM COMMERCIAL BROILER CHICKEN(Indian Veterinary Association Kerala, 2015-11) Shilpa, P.; Kirubaharan, J. John; Aishwarya, S.; Chandran, Daniel Joy; TANUVASInfectious bronchitis (IB) is an economically important viral disease of chickens, caused by infectious bronchitis virus (IBV) belonging to the family Coronal/jrjdae. The single stranded RNA genome of this virus has enormous capacity to change both by spontaneous mutation and by genetic recombination resulting in emergence of variants. The present study involved isolation and charac- terisation of avian infectious bronchitis from broiler chicken with nephropathological lesions. Kidney sample was inoculated into 9-10 day old embryonated chicken eggs by allantoic route. Typical lesions of IB like curling and dwarfing of embryos was evident after three passages. The presence of virus was confirmed by reverse transcription PCR of 3’UTR region. Complete spike protein was sequenced and the sequence analysis revealed that the isolate belong to M41genetic groupThesisItem Open Access RECOVERY OF AVIAN INFECTIOUS BRONCHITIS VIRUS FROM C DNA (B17 STRAIN)(TANUVAS, Chennai, 2017) Shilpa, P.; Kirubaharan, J. John; TANUVAS; Thangavelu, A.; Kumanan, K.; Ganesan, P.I.