Browsing by Author "Sharma, Sudhir"
Now showing 1 - 3 of 3
Results Per Page
Sort Options
ThesisItem Open Access Heterosis and combining a hility analysis in maize (Zeamays L.)(College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 2001) Sharma, Sudhir; Narwal, M. SThesisItem Open Access Phenotypic and molecular characterisation of backcross generations for salt tolerance in wheat (Triticum aestivum L. em.Thell)(CCSHAU, 2017) Sharma, Sudhir; Vikram SinghSoil salinity is a major abiotic stress having adverse effects on all growth stages of wheat. In present study, HD 2967 x Kharchia 65 derived F3, BC1F2 (initial salt stress) and BC2F1 (under and field conditions) generations were evaluated for various morpho-physiological traits. A total of 430 seeds out of 750 BC1F2 seeds (57.33%) and 456 out of 700 F3 seeds (65.14%) germinated under salt stress. In all the three generations, ample variation was observed for all traits under study. Biological yield per plant, number of effective tillers per plant, spike length and plant height showed positively significant correlation with grain yield per plant across the generations. Path analysis of all three generations revealed that biological yield and harvest index had high direct effect on grain yield per plant while other traits viz. number of effective tillers per plant, plant height and spike length had significant indirect effect on grain yield per plant through biological yield per plant and harvest index. Marker assisted selection was done for Nax loci; responsible for salt tolerance in wheat. Thirty-seven plants, comprising of 29 BC1F2, 2 F3 and 6 BC2F1 showed amplification for both Nax1 and Nax2 locus. SSR (33) were used to generate allelic profile of selected plants. Molecular analysis showed that average recurrent parent recovery in BC1F2, BC2F1 and F3 plants was 83.18, 81.82 and 86.24%, respectively. Cluster tree analysis showed that HD 2967 and Kharchia 65 were clustered separately and plants in all three generations were clustered near recurrent parent HD 2967. Nine plants selected on the basis of high yield and the presence of Nax loci viz. plant number 50, 11 and 39 of F3, 16, 68 and 95 of BC1F2, and 21, 16 and 11 of BC2F1were advanced in the next generation and will be further backcrossed with the recurrent parent HD 2967 to develop salt tolerant wheat lines.ThesisItem Open Access Studies on genetic variability and association analysis for various morphological and quality traits in sugarcane (Saccharum spp.Complex) clones(CCSHAU, Hisar, 2021-09) Hardeep; Sharma, SudhirIn present investigation, 50 sugarcane genotypes were evaluated in randomized block design with three replications at the research farm of CCS HAU, RRS, Uchani, Karnal during Spring 2020. This study was intended to determine genetic variability, correlation and path analysis for seventeen characters among fifty early maturing sugarcane clones. Analysis of variance showed that mean sum of squares due to genotypes were highly significant for all the characters studied, indicating the presence of sufficient genetic variability. Higher estimates of PCV than corresponding GCV, thereby, revealing that there is slight influence of environment on different traits. The narrow difference between the GCV and PCV values indicated the greater role of genetic factors on the expression of characters than the environmental factors. High heritability in association with high genetic advance as percentage of mean was observed for number of tillers at 120 DAP, number of shoots at 240 DAP, single cane weight, cane length, cane yield and CCS (t/ha) revealed importance of additive gene action and and selection for these characters will be effective for further improvement in cane yield. Cane yield showed significant and positive association with CCS (t/ha), single cane weight, cane girth, cane length, number of millable canes at harvest, number of tillers at 120 DAP and number of shoots at 240 DAP whereas, CCS (t/ha) exhibited positive and significant correlation with cane yield, single cane weight, cane girth, cane length, number of millable canes at harvest, number of tillers at 120 DAP, number of shoots at 240 DAP, brix % at 8th and 10th month, pol % at 8th month and CCS % at 8th month suggests that for improving cane yield and sucrose content direct selection of clones based on positively associated characters will be more fruitful while planning any sugarcane breeding programme. The results of path analysis disclosed that the characters viz., CCS % at 10th month, CCS (t/ha) ,CCS % at 8th month, brix % at 8th month, number of millable canes at harvest, single cane weight, cane girth and number of tillers at 120 DAP recorded positive direct effects on dependent variable i.e. cane yield and the other traits also showed their indirect positive effects on cane yield through these characters, indicating that these characters are the major contributing traits to cane yield in sugarcane. Some sugarcane clones viz. 15-365, 15-1305, 10-31 and 15-1784 were identified in present investigation. These were significantly or numerically superior over best check Co 0238 in terms of cane yield (t/ha), commercial cane sugar (t/ha), pol % at 10th month and CCS % at 10th month. These sugarcane clones needs to be rigorously tested in mutilocational trials to identify suitable genotype to sustain sugarcane productivity.