Browsing by Author "SURENDER SINGH"
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ThesisItem Open Access Cellulase Production from Agro-residues(2008) SURENDER SINGHThe present investigation deals with the production of cellulase enzyme using agricultural residues (Paddy straw and sugarcane) as substrates which are available in plenty in agrarian country like India. Four microorganisms viz. Aspergillus awamori, Phanerochaete chrysosporium, Trichoderma reesei and Streptomyces aburaviensis were screened for their potential to produce hydrolytic enzymes especially cellulase on paddy straw and sugarcane bagasse using solid state fermentation. Solid state fermentation parameters like temperature, pH, inoculum size, moisture content and incubation time were optimized using most promising combination of the agro-residue and microorganisms for maximum cellulase production and finally the cellulase was purified from the crude filtrate and characterized. Aspergillus awamori among microorganisms and paddy straw between the two substrates showed significantly higher activities for most of the enzymes studied specially cellulases. Aspergillus awamori showed maximum cellulase enzyme production at pH 5.5 with paddy straw of 1.0 mm particle size at 35 °C after 8 days of incubation with 30 % inoculum when substrate: water ratio of 1:2 was used for moistening of substrate. Endoglucanase from Aspergillus awamori was purified employing a two steps process; ammonium sulphate precipitation followed by DEAE-Anion exchange chromatography. The purified monocomponent cellulase produced in the study comparatively has lower Km value, active over broad range of pH (4-9) and is thermostable which indicates its potential for commercial use in detergent industry and textile industry where monocomponent enzyme formulations of fungal endoglucanases are preferred. lkjka'k izLrqr v/;;u] Hkkjr tSls