Browsing by Author "SHRUTHI, B. T."
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ThesisItem Open Access CO-EXPRESSION OF REGULATORY GENES OsAlfin1, PgHSF4, PDH45 AND PsAKR1 TO IMPROVE ROOT GROWTH AND ABIOTIC STRESS TOLERANCE IN AEROBIC RICE(UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2015-07-14) SHRUTHI, B. T.; chandrasekara, ReddyDrought stress is one of the major limitations for sustained crop productivity. Plants evolved two distinct mechanisms under stress. The first one is maintaining cell turgor by improved water mining which also facilitates positive carbon balance. The other complex mechanism is to maintain cellular level tolerance under decreased cell turgor. The school of thought is that these two mechanisms are complementary and crucial to achieve desired level of tolerance. This can be achieved by pyramiding of genes regulating these traits. In this context, an attempt has been made to manipulate developing transgenics to improve adaptation under abiotic stress condition by coexpressing OsAlfin1 involved in root growth and PgHSF4, PDH45, PsAKR1 involved in protein synthesis and protection. The three genes were initially cloned into a suitable binary vector pi12GW harbouring PsAKR1 using gateway cloning strategy. Initially, In planta transformation method was used to develop transgenics. T0 seeds were stringently screened for glyphosate resistance, the positive transformants were advanced to T1. Subsets of T1 seeds were screened against salinity, temperature, moisture stress and selectable marker glyphosate. Putative transformants were identified by all screening approaches after confirming integration of all genes by PCR analysis. The PCR positive transgenic plants showed increased root growth under normal condition, showed less membrane electrolyte leakage against MV stress and maintained higher chlorophyll stability under salinity stress and control condition compared to wild type. These data suggest that co-expression of OsAlfin1, PgHSF4, PDH45 and PsAKR1 can give tolerance to abiotic stress; indicating its significance in stress tolerance.