Browsing by Author "Reena, D"
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ArticleItem Open Access Effect of Hyaluronan Enriched Transfer Medium on Conception Rate in Bovine Embryo Transfer(2020-04) Reena, D; Thirupathi, R; Manokaran, S; Rangasamy, S; et al.; TANUVASThe present study was conducted to assess the effect of hyaluronan (HA) supplementation in the embryo transfer medium of in vivo produced, vitrified and warmed embryos on conception rate in bovines. The study was conducted in 12 recipient cows which were randomly divided into two groups viz. Group I (embryo transfer media with hyaluronan, experimental group) and Group II (embryo transfer media without hyaluronan, control group). The vitrified and warmed embryos were suspended in the embryo transfer medium according to the group, cultured and transferred to the synchronized recipients. The pregnancy diagnosis after 60 days revealed 50 and 33.33 per cent conception rate in Group I and II recipients, respectively. From the study it is evident that the enrichment of hyaluronan in the embryo transfer medium has beneficial effect on enhancing the conception rate in bovines.ArticleItem Open Access Effect of L-proline Supplementation in Vitrification Media on Post Thaw Survival and Embryonic Development of Sheep Oocytes(2021) Reena, D; Lakshmi, G; Kurunchi Malar, S; Manokaran, S; TANUVASThe study was conducted to explore the cryoprotective effect of L-proline on sheep oocyte vitrification. In vitro matured sheep oocytes (n=836) were vitrified by open pulled straw method in vitrification solution consisting of 15 per cent (v/v) DMSO+ 15 per cent (v/v) EG + sucrose (0.5M) + 20 per cent FBS in TCMh (Group I) and 7.5 per cent (v/v) DMSO + 10 per cent (v/v) EG + sucrose (0.5M) + 20 per cent FBS in TCMh supplemented with 2M L-proline (Group II). The oocytes were warmed and observed for viability by tryphan blue staining. The mean ± SE live oocyte percentage was significantly higher in group II than in group I (59.80±1.02 vs. 44.99±1.34). The vitrified thawed oocytes were activated parthenogenitically using ionomycin and 6-DMAP and developmental stages of embryos were analysed. In parthenogenetically activated embryos, there was no significant difference was observed in cleavage rate (47.56±2.09 vs/ 42.45±3.44) and morula (14.90±1.68 vs. 12.95±2.25) percentage between L-proline and control group. From the study it is concluded that L-proline could be used as a cryoprotectant with high efficiency in sheep oocyte vitrification.OtherItem Open Access Effect of Long-Term vs Short-Term Neem Feeding on Semen Quality of Tellicherry Bucks(TANUVAS, 2010) Muralidharam, J; Ranganathan, V; Reena, D; Thiruvenkadan, A.K.; Karunanithi, KArticleItem Open Access Effect of Two Different Culture Media on Developmental Rate of Bovine Embryos invitro(2020-06) Sanjeeva Kumar, GSR; Reena, D; Manokaran, S; Balasubramanian, S; TANUVASIn the present study, effect of two different culture media on the developmental competence of bovine oocytes was studied. For the study a total of 1110 oocytes were collected using ovum pick-up (OPU) technique and the average oocyte yield was 8.34 ± 0.23 per OPU session per animal. The oocytes were graded as A, B, C and D grades based on the layers of compact cumulus cells surrounding the zona pellucida. Among this only A, B and C grade oocytes were used for in vitro maturation. The oocytes were matured in TCM 199 media supplemented with 10 per cent FBS, 1 g/ml Folltropin (Bioniche, Canada), 0.02 IU/ml LH, 1 g/ml estradiol with addition of 100 μM cysteamine, 10 ng/ml EGF, 100 ng/ml IGF, 10 μg/ml insulin, 5.5 μg/ml transferrin and 5 μg/ml selenium and the overall maturation rate was 91.30± 1.27 per cent. The presumptive zygotes were cultured in two different culture media viz. i) two step sequential synthetic oviduct fluid (SOF) media and ii). single step potassium simplex optimised medium (KSOM). The mean (± SE) cleavage, 4 cell, 8 cell and morula development rate was 73.04 ± 1.50 and 79.01 ± 1.34 per cent, 54.88 ± 1.76 and 60.69 ± 1.89 per cent, 35.89 ± 1.57 and 44.67 ± 1.62 per cent and 19.18 ± 1.10 and 25.36 ± 1.37 per cent in SOF media and KSOM, respectively. Based on the cleavage and embryo development observed, it was concluded that KSOM could be better than SOF media for in vitro production of bovine embryos.ThesisItem Restricted EXPRESSING THE INFLUENCE OF EPIDERMAL GROWTH FACTOR AND LEUKEMIA INHIBITORY FACTOR IN THE DEVELOPMENT OF BOVINE PREIMPLANTATION EMBRYOS(2018) Vinayak, Sadekar Gautami; Meenambigai, TV; Mangalagowri, A; Reena, D; TANUVASAn array of growth factors and cytokines are associated in the development of embryos under in vivo and in vitro conditions. These growth factors and cytokines have autocrine and paracrine cell-cell signaling actions that contribute to yielding healthier embryos. Their actions in the embryo include modulation of cell gene expression and metabolic function that in turn influence cell survival and differentiation, ultimately impacting embryo implantation competence and post-implantation development. The effect of Epidermal Growth Factor (EGF) and Leukemia Inhibitory Factor (LIF) in the development of IVM/IVF bovine oocytes /embryos and their gene expression analysis were investigated in this study.ArticleItem Open Access Intriguing Role of Lysophosphatidic Acid (LPA) and its Receptor Mediated Signaling during Implantation: A Review(2020-06) Madhumitha, B; Reena, D; Manokaran, S; TANUVASRegardless of major advances in medical technologies many Assisted Reproductive Technology (ART) experience recurrent implantation failures (RIF). Lysophosphatidic acid (LPA) signaling cross talks between the mother and the implanted embryo at a very early stage of gestation to enhance endometrium receptivity. LPA is a simple water soluble phospholipid that arbitrates varied biological functions like proliferation, migration and activation of various intracellular signaling pathways in diverse cell types. LPA also has a chief role to play in human and animal reproductive processes including luteolysis, endometrium and ovarian function, estrous cycle regulation, embryo development and implantation, placentation and decasualization with the help of receptor (LPAR1-6) mediated LPA signaling. Further, autotaxin a LPA producing enzyme is found to be upregulated during decasualization of human endometrial stromal cells (HESC). LPA aids in the maintenance of endometrium receptivity by invigorating the expression of prostaglandin endoperoxide synthase 2 (PTGS2). LPA also increases progesterone (P4) and prostaglandin E2 (PGE2) secretion and elevation in PGE2/PGF2α ratio. This review highlights and discusses about the current advancement in receptor mediated LPA signaling in human reproduction, ruminant reproduction pointing to bovine and sheep models, and even in porcine reproduction models and correlating it with human reproductive function.Book chapterItem Open Access NUTRITION Vs REPRODUCTION - MYTH AND REALITY(2014) Asokan, SA; Reena, D; Sankaran, VM; Vennila, C; Senthilkumar, T; TANUVASThe Indian bovine population is 304.42 million out of which 4.34% (13.20 million) are reared in Tamil Nadu (18th Livestock census, Animal Husbandry Department, 2007). Among the southern state of India, Tamil Nadu has emerged as a forerunner in milk production producing around 68.34 lakh tonnes of milk. This is primarily due to the high crossbred dairy cattle in total dairy cattle population has increased from 28.67 per cent in 1997 to 62.95 per cent in 2007 (Policy note, 2012-13).ArticleItem Open Access Osteopontin ameliorates sodium nitroprusside induced free radical damage on sperm motility of frozen thawed buffalo semen(2020) Viswam, Visakh; Loganathasamy, K; Gomathy, VS; Reena, D; TANUVASThe experiment was undertaken to study sodium nitroprusside (SNP) induced free radical damage on sperm motility of frozen thawed buffalo semen and the ameliorative effects of osteopontin (OPN) supplementation on sperm motility. Buffalo frozen semen straws from 8 ejaculates of 6 bulls were procured from Central Frozen Semen Production and Training Institute, Hessarghatta, Banglore and stored at Semen Bank, Madras Veterinary College, Chennai. The semen straws were thawed and seminal plasma and semen extender were removed from spermatozoa by centrifugation. Spermatozoa were suspended in 1mL capacitation medium (control), with the addition of 100μg/mL of OPN (treatment I) or 100μM/mL of SNP (treatment II) or 100μg/mL of OPN + 100μM/mL of SNP (treatment III). Semen with capacitation medium alone without any supplementation served as control. The contents were incubated at 37ºC for 4 h and the post capacitation sperm motility was observed under bright field microscopy. The post capacitation sperm motility was significantly (P<0.05) higher in treatment I (71.80% ± 0.04) as compared to control (61.71% ± 0.03), treatment II (24.82% ± 0.07) and treatment III (46.64% ± 0.05). But, the post capacitation motility in treatment II and III were significantly (P<0.05) lower than control. The post capacitation motility in treatment III was significantly (P<0.05) higher than treatment II. The study indicated that addition of SNP alone in the capacitation medium has detrimental effects on the sperm motility and addition of OPN alone in the capacitation medium exerts beneficial effects on sperm motility. When both OPN and SNP were added in the capacitation medium, OPN partially ameliorated the toxic effects of SNP on the sperm motility of frozen thawed buffalo semen.