Browsing by Author "Rathor, Ashok Singh"

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    STUDIES ON CLINICO-HAEMATOBIOCHEMICAL AND THERAPEUTIC EFFICACY OF POLYHERBAL FORMULATION AGAINST RESISTANT GASTRO INTESTINAL NEMATODES IN GOATS
    (G.B. Pant University of Agriculture and Technology, Pantnagar-263145, 2023-08-01) Rathor, Ashok Singh; Bhatt, Prakash
    The present study was conducted at goat farms/herds of Tarai region around Pantnagar. Total 146 goats exhibiting FAMACHA scores ranging from 3 to 5, were examined to determine the prevalence of GI nematodosis. Out of 146 goats, 103 were diagnosed with GI nematodosis. From these 103 goats, 24 goats of either sex, aged between 9 months to 5 years, were selected with egg counts exceeding 200. They were randomly assigned to four equal groups. Group I received oral fenbendazole treatment at 5mg/kg body weight, Group II received oral ivermectin at 0.2mg/kg body weight, Group III received an oral polyherbal formulation at 6.5g/30 kg body weight, and Group IV served as the untreated control. A pilot study was also conducted earlier on three goats to rule out any adverse effects of the polyherbal formulation used in the experimental study. The haematological, biochemical, and oxidative stress related parameters were investigated at intervals of 0, 7, 14, and 21 days. Faecal samples were collected directly from rectum on 0 day (Pre-Treatment), 7th day, 14th day and 21st Day (post-treatment) for calculation of EPG and FECRT. There was significant improvement in the mean values of Red Blood Cells, Haemoglobin, and Packed Cell Volume after treatment with polyherbal formulation and ivermectin, as compared to control. ALP, AST, and ALT levels within Groups I, II, and III reduced significantly by the 21st day, aligning with the healthy goat's standard range. Whereas the values in Group IV showed a steady rise. The increased enzyme activity in affected animals aligns with detrimental effects caused by traumatic abomasal and intestinal damage, directly linked to parasitic infestation. Serum concentrations of GSH-Px, SOD, and CAT were notably elevated on the 21st day post-treatment in goats treated with the polyherbal formulation, ivermectin, and fenbendazole, along with decreased MDA levels, indicating decreased oxidative stress after treatment. It was revealed that levels of GSH-Px, SOD and CAT were low during stress caused by GI nematodosis whereas MDA levels were high. Assessment of EPG and FECRT (%) values on the 21st day post-treatment revealed efficacy rates of 85.07% for ivermectin, 90.62% for the polyherbal formulation, and 59.05% for fenbendazole. Significant improvement in the mean EPG in both treatment groups G II and G III was seen on 14th day post treatment compared to G I thus indicating resistance to fenbendazole. Coproculture on the 14th day post-treatment indicated that 97% of identified species were H. contortus, establishing it as the primary cause of GI nematodosis in goats from the Tarai region of Uttarakhand.