Browsing by Author "Ramya, R"
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ThesisItem Open Access A COMPARATIVE STUDY OF CONENTIONAL SUTURE AND STAPLER ANASTOMOSIS FOR GASTRO-INTESTINAL SURGERY IN DOGS(TANUVAS, 2016) Ramya, R; Jayaprakash, R; TANUVAS; Ravi Sundar George; Vairamuthu, SThe study was carried out on 12 clinical cases of dogs that were brought to Madras Veterinary College Teaching Hospital, with a history of gastro-intestinal affections requiring surgical intervention. The cases were selected based upon inclusive and exclusive criteria respectively. Twelve dogs selected for the study were randomly divided into two groups of 6 each. Conventional suture method in group l animals and anastomotic stapler in group II animals with gastro-intestinal obstructions were performed.ThesisItem Open Access Design and development of artificial pollinizer for pollinating tropical vegetables under protected cultivation(Department of Farm Machinery and Power Engineering Kelappaji College of Agricultural Engineering and Technology, Tavanur, 2018) Ramya, R; KAU; Sureshkumar, P KArtificial pollination is a process that is highly require in vegetable crops grown under protected cultivation since, protected environment offers barriers to natural agents for pollination such as insects, wind or water. A study was undertaken to design and develop an artificial pollinizer for pollinating tropical vegetable crops under protected cultivation. To obtain preliminary data required for the design, floral and physical characteristics of flower, anther and pollen of selected tropical vegetable crops were studied in laboratory conditions. Accordingly a conceptual design was evolved and two models of artificial pollinizers were developed. Suitability of the developed pollinizer was evaluated in laboratory as well as field conditions. Results of the evaluation have been presented and analyzed. Six tropical vegetable crops were selected for the preliminary studies like floral characteristics, physical and dimensional measurements of flowers, anther and pollen grains were conducted in the laboratory. Sizes of pollen grains were measured by using Olympus Bx43 light microscope and the measurements were taken using the software Ultrascope version 9.1. Shape was determined from standard shape charts suggested by UPOV, 2007. The shape of pollen grains of tomato, pumpkin and ash guard are found to be „circular‟ with a Length/Width ratio ranging from 1: 1 to 1: 1.1. Pollen grain of chilli is „broad deltate‟ with L/W ratio of 1: 1.2. Pollen grains of water melon and cucumber are „narrow oblate‟ with a L/W ratio of 1: 1.13 to 1:1.15. Based on the preliminary studies, functional requirements and conceptual designs two models of the artificial pollinizers were designed and developed. Artificial pollinizer Model – I uses air as the medium for collection of pollen from flowers. Suction pressure developed by a vacuum pump suck the pollen grains from the male flower to a pollen collection chamber. The pollen collection unit is provided with a brush tip which detach the pollen grains from the flower and sucked by a vacuum pump are deposited in a pollen collection chamber. A screen mesh filter of 15 μm aperture is used in the chamber to prevent the pollen grains from carry away. Components of artificial pollinizer model – I includes pollen collection unit, pollen collection tube, adaptor for pollen collection chamber, pollen collection chamber, air tube, air tube adaptor, a connector and a vacuum pump. An electric powered air blower of 220 V, 50 Hz and 0.55kW is used as vacuum pump. The blower produces a suction velocity of 20-22 ms-1 at 13,000 rpm. Artificial pollinizer Model – II uses water as the medium for collection of pollen from male flowers. Water is sprayed from a nozzle to the male flower kept carefully in a pollen collection chamber. Water spray produced by a pneumatic hand pump from the nozzle wash out the pollen grains from the flower and is collected in the water tight container. Components of artificial pollinizer Model – II includes a spray nozzle, pneumatic hand sprayer, water container and pollen collecting chamber. Collected pollen can be sucked by a syringe for spraying to the female flower for artificial pollination. Dry pollen collected by Model – I is also mixed with water and used for artificial pollination using a syringe. Evaluation of artificial pollinizer was done by accessing pollen viability and fruit set efficiency. Viability of pollen was observed under laboratory condition and fruit set efficiency was observed under field conditions. From the studies it is observed that, pollen viability is decreasing with increase in storage period for both the crops. Maximum pollen viability is observed when artificial pollination was done with fresh pollen grains. Also, the viability is found higher for pollen stored in 1 % sucrose solution than pollens stored in plain water in both crops. Study on fruit set efficiency is also seems to be decreasing with increase in storage period for both the crops. Maximum fruit set efficiency is observed when artificial pollination was done with fresh pollen grains. Fruit set efficiency is higher for pollen stored in 1 % sucrose solution than pollens stored in plain water in both crops. Also the fruit set efficiency values are on par with manual pollination. Stages of fruit development in cucumber and watermelon crops are also observed on the 3 rd, 7th and 15th days after artificial pollination and found that the artificial pollination done using the artificial pollinizer was successful.ArticleItem Open Access EVALUATION OF A NEW ‘ON FARM TEST’ FOR DETECTION OF BOVINE SUBCLINICAL MASTITIS(2021-04) Ramya, R; Madhanmohan, M; TANUVASDiagnosis of subclinical mastitis is problematic as the milk and udder appears normal but generally has an elevated somatic cell count. In the present study, a new ‘on farm test’ kit “TANUCHEK SCC kit” was evaluated to diagnose subclinical mastitis. Efficiency of this ‘on farm test’ kit was initially compared with California mastitis test (CMT). Further, the results of these indirect tests (TANUCHEK SCC kit and CMT) were compared with the direct test i.e. direct microscopic somatic cell count which was used as reference. Milk samples (n=610) were simultaneously subjected to direct microscopic somatic cell count, CMT and TANUCHEK SCC kit for the presence of subclinical mastitis. The prevalence of subclinical mastitis was 80.33%, 72.95% and 68.85% for the direct test, CMT and TANUCHEK SCC kit, respectively. Kappa statistics was used to compare the results. The TANUCHEK SCC kit had showed substantial agreement (k = 0.8612) and moderate agreement (k=0.5749) with CMT and the reference direct microscopic somatic cell count, respectively. Results of this study revealed that the TANUCHEK SCC kit can be a simple, easily adoptable, on farm screening test to diagnose SCM in field condition.ThesisItem Open Access Influence of micro meteorological factors on flowering in vanilla (Vanila Planifolia Andrews)(College of Horticulture, Vellanikkara, 2008) Ramya, R; KAU; Shylaja, M RInvestigations on “Influence of micro meteorological factors on flowering in vanilla” were carried out at the Department of Plantation Crops and Spices, College of Horticulture, Kerala Agricultural University during 2005-2007. The objective of the study was to find out the effect of soil moisture stress and micro - meteorological factors on flowering in vanilla. The studies were carried out in five year old vanilla plants maintained in Department of Plantation Crops and Spices farm and in a selected farmer’s field at Thrissur district. Moisture stress was induced in vanilla gardens by withholding irrigation at four levels viz. one month, 1½ month, two months and 2½ months. The influence of soil moisture stress on flowering in vanilla and the changes in physiological and biochemical parameters in vanilla due to moisture stress and influence of micro meteorological parameters on flowering in vanilla were studied in the present investigations. Soil moisture stress induced flowering in vanilla. Maximum flowering of 80 per cent was observed in plants stressed for moisture for a period of one month followed by 60 per cent flowering in 1½ month stress period. Hence soil moisture stress for a period of 1-1½ month during November-December is sufficient to induce flowering in vanilla. Soil moisture stress altered various physiological and biochemical parameters in vanilla. Leaf thickness, relative leaf water content, membrane stability, soluble protein and total chlorophyll content decreased due to soil moisture stress. The content of epicuticular wax, total free amino acids, accumulation of proline, activity of peroxidase enzyme, total sugar content and K concentration in tissues increased due to stress. The changes in physiological and biochemical parameters were more pronounced as the intensity of stress increased. The micro-meteorological parameters of the garden also influenced flowering in vanilla. Flower opening in vanilla was found positively correlated with maximum temperature and negatively with relative humidity and minimum temperature. Flower opening showed highly significant positive correlation with light received from North and South directions and percentage of light infiltration. Soil moisture stress for a period of 1-1 ½ month during November- December is sufficient to induce flowering in vanilla. Changes in physiological parameters due to moisture stress could be used to visually assess the extent of moisture stress in the garden. The biochemical parameters recorded at ideal stress period could be used as indices to assess the extent of moisture stress in plants more precisely in high tech / precision farming systems. Manipulation of microclimate with respect to temperature, light and relative humidity is essential for getting proper flowering in vanilla.ArticleItem Open Access NITRATE POISONING IN DUCKS(2016-12) Premalatha, N; Ramya, R; TANUVASA study was carried out to determine the cause of sudden death of ducks at Mettukolathur farm, Kancheepuram district. To explore the cause of mortality; history and clinical signs were recorded. Post-mortem examination was carried out and samples were collected for laboratory diagnosis. The nitrate content of water was found to be 100mg/L. History along with clinical symptoms, tissue changes and laboratory analysis suggested nitrate poisoning. There may be possible relationship with flooding and toxic levels of nitrate since the mortality occurred immediate after floods in the area.ArticleItem Open Access Rabies Prophylactic Approaches(2018-03) Ramya, R; Madhanmohan, M; Nagarajan, T; TANUVASRabies is a fatal zoonotic disease of serious public health and economic significance around the globe. It is caused by a bullet-shaped virus belonging to the genus Lyssavirus of family Rhabdoviridae. India is one of the few rabies endemic countries that continue to face problems associated with large number of human deaths due to rabies. In the developed countries, human rabies has dramatically declined during the past 50 years as a direct consequence of routine vaccination of pet animals. However, wildlife rabies has emerged as a major threat. Therefore, rabies is preventable by controlling rabies in both wildlife and domestic animal populations. Vaccination remains the only viable alternative for prevention, control and eradication of rabies in both developed and developing countries. Since Louis Pasteur’s first attempt to produce rabies vaccines, a number of approaches have been evolved from the usage of nervous tissue vaccines to the novel recombinant vaccines. However, to date Rabies remains a global health threat despite it being a vaccine-preventable disease. This status clearly indicates a demand for more effective and economic rabies vaccines. The protein subunit based vaccines consisting the immunogenic components of a virus can be a methodology to produce the affordable, safe and immunogenic rabies vaccines without the necessity of handling live rabies virus.ArticleItem Open Access Therapeutic Management of Intranasal Transmissible Venereal Tumor in a Dog(2016-07) Sankar, P; Ramya, R; Mohamed Ali, MG; TANUVASA Mongrel dog was presented with history of persistent bilateral epistaxis and dyspnea. Physical examination revealed characteristic hard mass irregularly distributed in nasal cavity. Blood samples were collected for haematology and biochemistry examinations and found within normal limits. Based on clinical examination and fine needle aspiration cytology, the case was diagnosed as transmissible veneral tumor with abundant vacuolated cytoplasm containing fine nuclear chromatin pattern, large round cells with round nuclei. The dog was treated with intravenous administration of Vincristine sulphate in four weekly doses leading to complete regression of lesions.Book chapterItem Open Access கிராமப்பிரியா மற்றும் அசீல் கோழிகளில் இராணிகெட் நோய்க்கான வாய் வழி குருணை வடிவ தடுப்பு மருந்து பற்றிய ஓர் கள ஆய்வு(2017) Ramya, R; Premalatha, N; TANUVASBook chapterItem Open Access நாட்டுக்கோழிகளில் அஸ்கரிடியா உருளைப்புழு தாக்கமும் மற்றும் கோலிசெப்டிசீமியா உடன்வுறை நிகழ்வு(2017) Premalatha, N; Ramya, R; TANUVASBook chapterItem Open Access பூனைக் குட்டிகளில் ஐசோஸ்போரியாசிஸ் பற்றிய ஓர் ஆய்வு அறிக்கை(2017) Premalatha, N; Ramya, R; TANUVASBook chapterItem Open Access மடிநோய் கண்டறியும் மாஸ்டி டெஸ்ட் ஏபிஎஸ்டி களப்பரிசோதனை கிட்(2017) Ramya, R; Premalatha, N; TANUVAS