Browsing by Author "Raja, S. Satheesh"
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ArticleItem Open Access Detection of Campylobacter Jejuni in Chicken Meat by Polymerase Chain Reaction(Indian Veterinary Journal, 2017-02) Raja, S. Satheesh; Rao, V. Appa; Babu, R. Narendra; Raja, A.; Abraham, Robinson J.J.; Thamizhannal, M.; TANUVASA study was conducted to develop a PCR protocol for detection of Campylobacter jejuni in chicken meat. DNA was extracted by using commercial DNA extraction kit, Phenol Chloroform and boiling method. Samples with OD ratio (260:280) between 1.7 and 1.9 were considered good in terms of concentration and purity and were used for PCR amplication using the genus specic primer was designed by targeting the Hyp gene of C.jejuni. Electrophoresis of amplied PCR products and gel documentation revealed 500 bp in 2% Agarose. The PCR technique was standardized using the reference strains and the minimum detection level (sensitivity) by PCR for Campylobacter jejuni was found to be 0.2 ng and it can detect C.jejuni upto 3 x 105 of meat homogenate. Hence, it is one of the rapid screening method for detection of C.jejuni in chicken meat within 24 hours.ThesisItem Open Access Detection of Emerging Food Pathogens in Chicken Meat Using Multiplex Polymerase Chain Reaction(TANUVAS, 2012) Raja, S. Satheesh; TANUVAS; Rao, V. Appa; Babu, R. Narendra; Raja, A.OtherItem Open Access Development of Biodegradable Packaging Material for Chicken Meat Packaging Using Coconut Shell Powder(TANUVAS, 2016-02) Thamizhnnal, M.; Rao, V. Appa; Abraham, Robinson, J.J.; Palanidorai, R.; Ruban, Wilfred; Raja, S. SatheeshArticleItem Open Access Development Of PCR Based Protocol For Detection Of Listeria Monocytogenes From Chicken Meat(2016) Raja, S. Satheesh; Rao, V. Appa; Thamizhannal, M.; Babu, R. Narendra; TANUVASA study was undertaken to develop a PCR protocol for detection of Listeria monocytogenes in chicken meat. Samples with OD ratio (260:280) between 1.7 and 1.9 were considered good in terms of concentration and purity and were used for PCR amplification using genus-specific primers were designed by targeting the prfA gene for Listeria monocytogenes. Electrophoresis of amplified PCR products and gel documentation revealed 290 bp in 2% Agarose. The PCR technique was standardized using the reference strains and the minimum detection level (sensitivity) by PCR for Listeria monocytogenes was found to be 0.1 ng. The developed PCR technique could detect Listeria monocytogenes upto 3 x 104 CFU/ml of meat homogenate. Thus the developed PCR technique can be used as a rapid screening test for detection of Listeria monocytogenes from chicken meat within 24 hours.ArticleItem Open Access Evaluation of Mechanical Properties of Biodegradable Packaging Materials(2016-08) Thamizhannal, M.; Rao, V. Appa; Raja, S. Satheesh; Ramakrishnan, C.; TANUVASA study was conducted to evaluate the mechanical properties of biodegradable packaging materials. The different packaging materials viz. Areca Sheath trays (T1) and Coconut shell trays (T2) and commercially available Styrofoam trays (control) were used and the mechanical properties of packaging materials (Tensile strength (MPa), Maximum Force (%), Elongation at Maximum (%), Elongation (%) were assessed.ArticleItem Open Access Screening Of Chicken Meat For Listeria Monocytogenes By Polymerase Chain Reaction In Chennai(2016-08) Raja, S. Satheesh; Rao, V. Appa; Babu, R. Narendra; Aravinth, A.; Thamizhanna, M.; TANUVASA study was conducted to identify the presence of L.monocytogenes in chicken meat by polymerase chain reaction. 40 samples were collected from different areas of chennai and were tested for the presence of L.monocytogenes by targeting prfA gene with 290bp by PCR. None of the samples were shown to be positive for L.monocytogenes. The test reveals that the processing can be performed hygienicallyArticleItem Open Access Shelf Life of Chicken Drumsticks Stored in Biodegradable Packaging Materials(Indian Veterinary Journal, 2017-01) Thamizhannal, M.; Rao, V. Appa; Abraham, Robinson J.J.; Palanidorai, R.; Raja, S. Satheesh; TANUVASA study was conducted to evaluate the Shelf life of chicken drumsticks stored in biodegradable packaging materials at 4±1C°. The pH, Extract release volume (ERV) and Total viable count (TVC) were assessed periodically at 0, 1, 3 and 5 days interval. The analysis of variance revealed a significant (p<0.05) increase in pH and Total viable count, but significant (p<0.05) decrease in Extract release volume with increase in storage period.