Browsing by Author "Prabhavathy, H"
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OtherItem Open Access ABILITY OF INDIAN STREET RABIES VIRUS ISOLATES TO INDUCE APOPTOSIS BY IN- VITRO(2015-01) Prabhavathy, H; Palanivel, KM; TANUVASAn attempt had been made in this study to know the ability of Indian Street rabies virus isolates to induce apoptosis in murine neuroblastoma cells. Thirty Indian street virus isolates were tested by FAT among which 20 samples were positive which were used to infect MNA cells. Four street rabies virus isolates and CVS were used in this study to show their ability to induce apoptosis. CVS used as control in the amplification as well as in the demonstration of apoptosis. The expression of Caspase- 1, Bad and glycoprotein genes in the MNA cells in comparision with CVS was measured by using densitometer. Street virus isolates expressed higher levels of Caspase-1 and Bad in comparison with CVS. Further TUNEL staining method showed higher apoptotic index when compared to CVS by Flow Cytometer reading. This study is revealed that Indian rabies street virus isolates can induce apoptosis in murine neuroblastoma cells.ArticleItem Open Access Ability of Indian street rabies virus isolates to induce apoptosis by in-vitro(2015) Prabhavathy, H; Palanivel, KM; TANUVASAn attempt had been made in this study to know the ability of Indian street rabies virus isolates to induce apoptosis in murine neuroblastoma cells. Thirty Indian street virus isolates were tested by fluorescent antibody technique (FAT) among which 20 samples was positive. These positive samples were used to infect murine neuroblastoma (MNA) cells. Four street rabies virus isolates and challenge virus strain (CVS) were used in this study to show their ability to induce apoptosis. Total RNA was isolated and cDNA was synthesized by using gene specific primers pertaining to Caspase-1, BAD (Proapoptotic gene) and Glycoprotein gene of rabies virus. Challenge Virus Standard has been used as control in the amplification as well as in the demonstration of apoptosis. The expression of Caspase-1, BAD and glycoprotein genes in the murine neuroblastoma cells in comparision with CVS was measured by using densitometer. Street virus isolates expressed higher levels of Caspase-1 and BAD in comparison with CVS. Further TUNEL staining method showed higher apoptotic index when compared to CVS. This study is an initial attempt to exhibit the ability of Indian rabies street virus isolates to induce apoptosis in murine neuroblastoma cells (in vitro).ArticleItem Open Access Clinical Pathology and Management of Canine Ehrlichiosis(2019) Prabhavathy, H; Joseph, Cecilia; TANUVASThirty five (35) ehrlichiosis affected dogs exhibited symptoms of fever (90%), epistaxis (50%), popliteal lymph node enlargement (60%), respiratory distress (80%), anorexia (95%), anemia (71%), dullness (80%), depression (70%) with tick infestation or previous history of tick infestation. Examination of Giemsa stained peripheral thin blood smears revealed 16.12% (35/35) positivity for morulae of Ehrlichia canis. Hematological parameters showed monocytosis (91.42%) and thrombocytopenia, activated platelets (17.14%) and hypochromasia (42.85%). All dogs were treated with Doxycycline for twenty one days along with supportive therapy. Complete recovery was noticed in all after a month of treatment and recurrence of infection was also noticed in 2/35 (5 percent) within six months after therapy.ArticleItem Open Access A Study on Prevalence of Canine Leptospirosis in Chennai(TANUVAS, 2018) Prabhavathy, H; Joseph, Cecilia; TANUVASLeptospirosis, a zoonotic bacterial disease prevalent in Chennai was taken for the study with 200 serum samples were collected from vaccinated and non-vaccinated dogs. Microscopic agglutination test showed 95 percent of the samples positive results for Leptospira of which 52.63 percent cases showed positive agglutination to one serovar and 47.36 percent cases showed positive agglutination to more than one serovar. Though the dogs were vaccinated with strains of serovars of L. canicola, L. icterohaemorrhagiae, L. pomona and L. grippotyphosa only. This study also showed that the non-vaccinated strains of L. autumnalis and L. australis showed high prevalence as compared to other serovars in vaccinated and un-vaccinated dogs.