Browsing by Author "Pawar, Rahul Mohanchandra"
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ArticleItem Open Access Complete Genome Sequences of Lineage IV Peste des Petits Ruminants Viruses from the Indian Subcontitent(GenomeA, 2015-09) Masdooq, Aliyu Abdullah; Pawar, Rahul Mohanchandra; Parthiban, R. Aravindh Babu; Ragavendhar, K; Sundarapandian, G; Thangavelu, A; Raj, G. Dhinakar; TANUVASPresentationItem Open Access Correlation Between Signaling Lymphocyte Activation Molecule Expression and Peste Des Petits Ruminants Virus Replication in Mononuclear Cells of Different Species of Animals(TANUVAS, Chennai, 2007-08) Pawar, Rahul Mohanchandra; Raj, G. Dhinakar; Raja, A.; Thangavelu, A.; Balachandran, C.ArticleItem Open Access Detection and molecular characterization of ascarid nematode infection (Toxascaris leonina and Toxocara cati) in captive Asiatic lions (Panthera leo persica)(2012) Pawar, Rahul Mohanchandra; Lakshmikantan, Uthandaraman; Hasan, Shakir; Poornachandar, Anantula; Shivaji, Sisinthy; TANUVASThe objective of this study was to investigate the ascarid infection in Asiatic lions using scat samples, based on microscopic analysis, PCR amplification of the ITS-2 region of ribosomal DNA and sequence analysis of the amplicons. Microscopic analysis indicated the presence of eggs of Toxascaris leonina in eleven of the sixteen scat samples analysed and in one of these eleven scats eggs of Toxocara cati were also detected. In five of the scats eggs were not detectable. The presence of T. leonina in all the infected samples was also confirmed by PCR amplification of the ITS-2 of ribosomal RNA gene and five of these also showed amplicons corresponding to T. cati, respectively. Toxocara canis infection was not observed in any of the scat samples. Nucleotide sequence analysis of the ITS-2 region indicated 97% to 99% similarity with T. leonina and T. cati, respectively. To our knowledge, this is the first molecular characterization of ascarid infection in captive Asiatic lions from a zoological garden of India. This study also indicates that Asiatic lions are more prone to infection either with T. leonina or T. cati and the parasite is not host specific.ArticleItem Open Access Effect of siRNA Mediated Suppression of Signaling Lymphocyte Activation Molecule on Replication of Peste Des Petits Ruminants Virus in Vitro(Virus Research, 2008) Pawar, Rahul Mohanchandra; Raj, G. Dhinakar; Kumar, T.M.A. Senthil; Raja, A.; Balachandran, C.; TANUVASOtherItem Open Access Effect of Small Interfering RNA- Mediated Inhibition of Signalling Lymphocyte Activation Molecule on Peste Des Petits Ruminants Virus Replication in In-vitro(TANUVAS, Chennai, 2007-08) Pawar, Rahul Mohanchandra; Raj, G. Dhinakar; Senthilkumar, T.M.A.; Nainar, A. MahalingaArticleItem Open Access Expression Profile of Toll Like Receptors in a Range of Water Buffalo Tissues (Bubalus bubalis)(Veterinary Immunology and Immunopathology, 2008) Vahanan, B. Mayil; Raj, G. Dhinakar; Pawar, Rahul Mohanchandra; Gopinath, V.P.; Raja, A.; Thangavelu, A.; TANUVASArticleItem Open Access Isolation and Molecular Characterization of Canine Distemper Virus from India(Trop Anim Health Prod, 2011) Pawar, Rahul Mohanchandra; Raj, G. Dhinakar; Gopinath, V.P.; Ashok, A.; Raja, A.; TANUVASArticleItem Open Access Molecular Characterization of Hepatozoon spp. infection in Endangered Indian wild felids and Canids(2011) Pawar, Rahul Mohanchandra; Poornachandar, Anantula; Srinivas, Pasham; Rao, Kancharapu Ramachandra; Lakshmikantan, Uthandaraman; Shivaji, Sisinthy; TANUVASHepatozoon species are parasites that infect a wide variety of domestic and wild animals. The objective of this study was to perform the molecular detection and characterization of Hepatozoon spp. in Asiatic lion, Indian tiger, Indian leopard, Indian wild dog, Indian domestic dog and cat based on partial 18S rRNA gene sequences from Hepatozoon spp. in the naturally infected animals. Hepatozoon spp. could be detected in blood samples of 5 out of 9 Asiatic lions, 2 out of 5 Indian tigers, 2 out of 4 Indian leopards and 2 out of 2 Indian wild dogs and, 2 out of 4 domestic cats and 2 out of 3 domestic dog samples by PCR. Sequencing of PCR amplicon and BLAST analysis of partial 18S rRNA gene sequences indicated that the Hepatozoon spp. in Asiatic lion, Bengal tiger, Indian leopard and domestic cat was Hepatozoon felis (98–99% similarity) and in the Indian wild and domestic dog the phylogenetic neighbour was Hepatozoon canis (97–100% similarity). Presence of H. felis and H. canis in both domestic and wild animals suggested that they are not host specific and the same parasite causes infection in domestic and wild felids and canids in India and from different parts of the world. To our knowledge, this is the first report on detection and molecular characterization of H. felis infection in Asiatic lions, Indian tigers, Indian leopards and H. canis in Indian wild dog. Hepatozoon spp. may be a potential pathogen and an opportunistic parasite in immuno-compromised animals and could thus represent a threat to endangered Indian wild felids and canids.