Browsing by Author "Palanisamy, A."
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PresentationItem Open Access Accessory Corpus Luteum: Induction and Characterisation in Crossbred Cattle(TANUVAS, Chennai, 2007-08) Satheskumar, S.; Palanisamy, A.; Prasad, Ch. Srinivasa; Nainar, A. MahalingaThesisItem Open Access Blood Biochemical Characteristics of Canine Dermatitis(TANUVAS, 2003) Zarina, Aziz; TANUVAS; Thangavel, A.; Ahamed, N. Mushtaq; Palanisamy, A.ArticleItem Open Access Characterization of Blastomeres derived from different stages of in vitro produced buffalo (Bubalus bubalis) embryos to determine the Stemness/ Pluripotential ability(2012-10) Chigurupati, Srinivasa Prasad; Palanisamy, A.; Satheshkumar, S.; Gomathy, V.S.; Raj, G. Dhinakar; Thangavel, A.; TANUVASThe present study was carried out to study the expression of genes for pluripotency Oct-4 and Sox-2 in blastomeres, derived from various stages of in vitro produced buffalo embryos, for determination of the stem cell characteristics or pluripotential ability. 0.5 per cent pronase was used for zona lysis and subsequent isolation of embryonic stem (ES) cells. RT-PCR was performed by using reverse specific primer (3’ primer) for the first strand synthesis. Strong Oct-4 and Sox-2 expression were consistently observed in cells, obtained from 16-cell stage embryos, morula and blastocyst, whereas in cells from 2, 4 and 8-cell embryos (pre ZGA) Oct-4 and Sox-2 expression was low and variable. Out of eight trials, in three trials the blastomeres of 2-cell, 4-cell and 8-cell stage embryos (Pre ZGA) did not express Oct-4 gene and in five trials a faint band was observed. With regard to Sox-2 gene expression, in four of six trials the blastomeres of 2-cell, 4-cell and 8-cell stage embryos (Pre ZGA) a faint band was observed, but in two trials Sox-2 gene was not expressed, eventhough they were believed to be totipotent. Low and variable expression of Oct-4 and Sox-2 genes in early stage embryos (pre-ZGA) might be related to the exhaustion of maternally-generated Oct-4 and Sox-2 transcripts and then their recovery appears to be via expression of zygotic transcripts, which takes place in buffalo embryos from 16 cell stages onwards. Epigenetic mechanisms might be the cause of the low levels of Oct -4 and Sox-2 expression after fertilization. The sequence results for Oct-4 (NCBI Ac. No: EU661360) and Sox-2 (NCBI Ac. No: EU661361) were analyzed by BLAST and it was found that Oct-4 had 88% - 97% homology and Sox-2 - 93% to 99% homology across the phylogeny with mouse being distant relative.ArticleItem Open Access Clinico-Haematological Evaluation of two Anaesthetic Regimens for Embryo Transfer in Goats(Indian Journal of Animal Research, 1994) William, B.J.; Kathiresan, D.; Leela, V.; Palanisamy, A.; Rao, G.D.; Nachimuthu, K.; TANUVASSurgical embryo transfer in goats require an ideal anaesthetic state which will facilitate maximum embryo recovery from donors and better pregnancy rate and kidding rate in recipients.PresentationItem Open Access Cloning and Expression Bovine Interferon Gamma(TANUVAS, Chennai, 2007-08) Thamizharasan, A.; Palanisamy, A.; Raj, G. Dhinakar; Sivaselvam, S.N.; Nainar, A. MahalingaThesisItem Open Access Cloning and Expression of Bovine Interferon ý Gene(TANUVAS, 2007) Thamizharasan, A.; TANUVAS; Palanisamy, A.; Dhinakarraj, G.; Sivaselvam, S.N.ArticleItem Open Access Cloning and expression of Vp2 protein of infectious bursal disease virus(2020) Kumari, C Sworna; Meenambigai, TV; Mangalagowri, A.; Palanisamy, A.; Sarathchandra, G.; TANUVASThe VP2 protein of highly virulent infectious bursal disease virus (hvIBDV) was cloned and expressed in pRSET A vector with high level of protein expression. VP2 is the host protective protein with major neutralizing epitopes was cloned and expressed in E. coli expression system BL21- DE3. The protein expression was evaluated by induction studies with IPTg and characterized by SDS PAGE and western blotting analysis. Thus, the present study focusing on expression of the immunodominant VP2 protein that may be used as a protein boost in DNA vaccine production strategy for enhanced immunity against IBDV infection in chickens in the near future.ArticleItem Open Access Comparative Analysis of Follicular and Luteal Dynamics in Oestrous Cycles of Buffaloes And Crossbred Cattle(2011-06) Satheshkumar, S.; Palanisamy, A.; Rangasamy, S.; Kathiresan, D.; Kumanan, K.; TANUVASThe ovarian follicular and luteal developmental patterns of Jersey crossbred cattle (n = 6) and Murrah graded buffaloes (n = 6) were compared to test the hypothesis that the reduced reproductive effi ciency in buffaloes when compared with crossbred cattle might be due to variations in follicular and luteal dynamics.ThesisItem Open Access Comparison of Slow Freezing and Open Pulled Straw Vitrification Methods for Cryopreservation of Bovine Embryos(TANUVAS, 2008) Ajmal, C.H.; TANUVAS; Rajasundaram, R.C.; Subramanian, A.; Palanisamy, A.PresentationItem Open Access Comparison of TCM-199 and SOF Media on In vitro Development of Ovine Preimplantation Embryos(TANUVAS, Chennai, 2007-08) Rangasamy, S.; Satheskumar, S.; Prasad, Ch. Srinivasa; Palanisamy, A.; Nainar, A. MahalingaThesisItem Open Access Early Transcriptional Pattern of HSP 70.1 Gene in In vitro Produced Buffalo Embryos(TANUVAS, 2008) Indira, R. Lilly; TANUVAS; Palanisamy, A.; Ramaswamy, V.; Raj, G. DhinakarArticleItem Open Access Effect of Cysteamine Supplementation in Semi Defined Media on In vitro Production of Buffalo Embryos(Indian Journal of Animal Reproduction, 2009-12) Palanisamy, A.; Rangasamy, S.; Satheshkumar, S.; Kumanan, K.; TANUVASArticleItem Open Access Effect of Cysteamine Supplementation in SOF Medium on in vitro Culture of Buffalo Embryos(Indian Veterinary Journal, 2011-08) Indira, R. Lilly; Palanisamy, A.; Meenambigai, T.V.; Ramaswami, V.; Brindha, K.; Kumanan, K.; TANUVASIn the present study, oocytes were collected from slaughter house ovaries, sliced, in vitro matured and fertilized with buffalo sperms processed by swim up technique. The presumptive zygotes were culture in SOF medium in control group and SOF with cysteamine in treatment group.ArticleItem Open Access The Effect of Different Proteolytic Enzymes on the Dissolution of the Zona Pellucida of In vitro Produced Buffalo (Bubalus Bubalis) Embryos(Buffalo Bulletin, 2010-03) Prasad, Ch. Srinivasa; Gomathy, V.S.; Palanisamy, A.; Raj, G. Dhinakar; Thangavel, A.; Kumar, S. Sathesh; TANUVASArticleItem Open Access Effect of GnRH Injection at Different Stages of Estrus Cycle on Fertility in Repeat Breeding Cows(Indian Veterinary Journal, 2009-12) Prabaharan, V.; Kulasekar, K.; Devanathan, T.; Palanisamy, A.; TANUVASRepeat breeder cows divided into three treatment groups (I to III) were treated with 10 µg Buserlin at the time of insemination, on day 5 and on day 11 post insemination respectively. The conception rate was 46.15, 23.07, 15.38 and 23.07 per cent in groups I, II, III and IV (control) group respectively. It is inferred that a single injection of 10 µg Buserelin at the time of artificial insemination improved the conception rates in repeat breeding cows.ThesisItem Open Access Effect Of Synchronization Of Follicular Wave Emeregence On Superovulation And Embryo Yield In Crossbred Cows(Tamil Nadu Veterinary and Animal Sciences University, 2009) Satheshkumar, S.; TANUVAS; Subramanian, A.; Veerapandian, C; Palanisamy, A.The present study was aimed at assessing the effect of GnRH analogue in synchronizing the follicular wave emergence in Jersey crossbred cattle and to study the effect of initiating FSH treatment (normal and reduced doses) after synchronizing the emergence of follicular wave on superovulatory response and embryo yield. Six healthy, non-lactating and regularly cycling Jersey crossbred cows aged between 5-6 yrs were utilized for the study. Initially, as a control study, the normal follicular wave pattern was ultrasonographically investigated in all the six cows. In experimental group, all the animals were injected with GnRH analogue (Buserelin acetate; 10 μg i.m.) on Day 6 of the cycle (Day 0 – oestrum) and follicular wave pattern was studied. All the six animals were subjected for four superovulatory treatments. Treatment1-Conventional: FSH (400 mg - Folltropin-V) treatment was initiated on Day 10; Treatment II – Gn-D8-400: FSH treatment (400 mg) was initiated on the day of GnRH synchronized follicular wave emergence (Day 8); Treatment III – Gn-D10-400: FSH treatment (400 mg) was initiated two days after synchronized follicular wave emergence (Day 10) and Treatment IV- Gn-D10-200: Similar to Gn-D10-400 group, but superstimulated with 200 mg. Monitoring the normal follicular wave pattern revealed that 77.8, 16.7 and 5.6 per cent of oestrous cycles exhibited three, two and four follicular waves respectively. The first wave dominant follicles (DF) were in a growth phase during Days 0.8 – 6.67 of the oestrous cycle and got deviated from the subordinate follicles by 3.30 – 3.67 days after emergence irrespective of the number of follicular waves succeeding the first one. Thus characteristics of the first wave DF was much predictable than that of the subsequent waves. Three peaks of FSH levels could be appreciated in three-wavecycles preceding the emergence of each follicular wave. When GnRH was administered on Day 6 of the cycle, the DF (10.83 ± 0.38 mm) ovulated in all the animals (100 %) in a mean interval of 27.67 ± 0.21 h and a synchronized homogenous group of follicles emerged after two days (Day of 8.00 ± 0.0). With the formation of an additional luteal structure (ACL), the progesterone concentration (10.57 ± 0.61 ng / ml) was significantly (P<0.01) increased than normal cycle (5.58 ± 0.45 ng / ml) during the mid luteal phase. In Conventional group, all the animals (100%) responded for FSH, with 13.67 ± 1.80 CL and 2.00 ± 0.37 AF. However, the superovulatory response varied widely (9 - 21ovulations). In Gn-D10-400 group, the animals responded with 11.00 ± 0.63 (10 – 14) CL and 3.50 ± 0.22 (3 – 4) AF comparable to the Conventional group. In both Conventional and Gn-D10-400 groups, almost all the Class III follicles on the day of superovulatory oestrus ovulated indicating that these follicles were healthy enough to respond to endogenous LH surge. In Gn-D8-400 group, the mean number of CL and AFs were 5.00 ± 1.77 (1 – 11) and 12.83 ± 4.65 (2 - 30) respectively. More number of Class II follicles and suprabasal progesterone levels (due to incomplete luteolysis) on the day of oestrus was correlated positively with increased number of AFs. The embryo recovery rate (53.89 %) in Gn-D10-400 was comparable to that of Conventional group (55.49 ± 9.70 %), but the recovery rate (36.57 ± 16.44 %) was substantially affected in Gn-D8-400 group. In Gn-D10-400 group, a higher percentage (87.24 %) of transferable quality (Grade 1 and 2) embryos and lower incidence of arrested / degenerated embryos (3.33 %) and UFO (1.85 %) was recorded than the Conventional (79.49%, 6.72 % and 6.25 % respectively) group. However, in Gn-D8- 400 group, arrested/degenerated and UFO (62.50%) and Grade 4 embryos (20.84%) constituted the major proportion of ova recovered and transferable quality embryos accounted for only 8.34 per cent. Increased anovulatory follicular population from the day of oestrus to the day of embryo collection and thus an increased concentration of oestradiol would have resulted in a poor embryo recovery / quality in Gn-D8 group. With the reduced dose of FSH (200 mg) in Gn-D10 group, all the animals responded with a mean superovulatory response of 6.33 ± 0.99 CL (4-11) and an average of 44.24 per cent embryos / ova were recovered. When compared with Gn- D8-400 group, a non-significant increase in superovulatory response and embryo recovery rate was recorded in Gn-D10-200 group.PresentationItem Open Access Embryonic Stem Cell Isolation and Characterization to Cellular Lineages(TANUVAS, Chennai, 2007-08) Gowri, A. Mangala; Nainar, A. Mahalinga; Balachandran, C.; Raj, G. Dhinakar; Palanisamy, A.ArticleItem Open Access Expression of Heat Shock Protein 70.1 Gene in In Vitro Developed Buffalo Embryos(Indian Vet. J., 2012-12) Indira, R. Lilly; Palanisamy, A.; Raj, G. Dhinakar; Meenambigai, T.V.; Kumanan, K.; TANUVAS
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