Browsing by Author "PRABHU, K. N."
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ThesisItem Open Access DOCUMENTATION AND MOLECULAR CHARACTERIZATION OF WILD MUSHROOMS FROM WESTERN GHATS OF KARNATAKA(UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2017-09-04) PRABHU, K. N.; Ramanjini Gowda, P. H.Western Ghats of Karnataka is one of the world’s biodiversity hot spot and has four major forest types, 23 floristic types and unique high altitude grasslands. The cool and humid climate make it ideal habitat for different kinds of mushrooms. In the present study, ethno-mycological survey was made in and around Chikkamagaluru, Dakshina Kannada, Hassan and Shivamogga districts of Karnataka for mushroom flora during June to October (2014-2016) with the help of local peoples and Adivasi tribes. Totally fifty mushrooms collected during the above period and identified. Out of fifty mushrooms, ten mushrooms were identified by morphological characters and forty mushrooms were identified by using Internal Transcribed Sequence (ITS) homology available in the National Centre for Biotechnology Information (NCBI) USA. The sequence alignment was made with NCBI data base revealed 85-100 % homology. The mushrooms viz., Schizophyllum commune, Tremella fuciformis, Lentinus sajor-caju (WGM-35), Lentinus sajor-caju (WGM-36), Lentinus polychrous, Lentinus strigosus, Termitomyces eurrhizus, Pleurotus pulmonarius, Pluteus petasatus and Polyporus tenuiculus were cultured using stipe tissue on Potato dextrose agar (PDA) and the Lentinus sajor-caju was cultivated on paddy straw and sawdust as substrate as it exhibited good mycelial growth. The highest bio-efficiency (65.25 %) was recorded in 50 % each Paddy straw and sawdust mixed substrate. The least bio-efficiency (38 %) was recorded in paddy straw substrate alone. The protein, fat and fiber content were in the range of 22.33-34.46 %, 1.8-4.0 % and 5-12 % respectively. Minerals viz., Ca, Mg, Fe, Zn and P were in the order of 48.45-143.96 μg/g, 14.78-59.49 μg/g, 8.44-33.32 μg/g, 0.58-3.4 μg/g and 107.33-505.66 μg/g respectively.