Browsing by Author "PARUL SHARMA"

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    IN VITRO ROOT REGENERATION IN KIWIBERRY [Actinidia arguta (Siebold & Zucc.) Planch. ex. Miq. var. Arguta]
    (UHF,NAUNI, 2023-12-30) KRITIKA KHAGTA; PARUL SHARMA
    ABSTRACT During the present investigation, the root regeneration in four cultivars viz. Annanasya, Dumbarton Oaks, Kens Red and Meader Male of kiwiberry (Actinidia arguta) via two approaches i.e., in vitro and ex vitro were followed. In vitro root regeneration was studied using two methods (onestep and two-step). In the former method, the best response for root regeneration was recorded in treatment RMO4 (1.00 mg/L IAA) as it produced callus free, good and healthy roots in all the four kiwiberry cultivars.The hardening of plantlets raised from one-step method was best in potting mixture, PMO5 [cocopeat + perlite + vermiculite in (3:1:1)] with respect to all the recorded parameters in case of all the four kiwiberry cultivars. In the two-step method, the best treatment for root regeneration was RMT2 (100.00 mg/L IBA) as it produced highest average number of roots per shoot in all the four kiwiberry cultivars. Meanwhile, for the hardening of plantlets raised from twostep method again the potting mixture PMO5 was the best with respect to all the recorded parameters in case of all the four kiwiberry cultivars. On the other hand, in the second root regeneration approach i.e., ex vitro, the treatment RME2 (100.00 mg/L IAA) resulted in maximum average per cent root regeneration and average per cent survival of the plantlets in all the four kiwiberry cultivars, respectively. Out of two root regeneration approaches followed, in vitro (one-step method) was proved better than ex vitro as it resulted in maximum average per cent root regeneration and maximum average per cent survival of plantlets. Overall, cv. Dumbarton Oaks performed well for root regeneration followed by cv. Meader Male. Thus, the present study developed an efficient and reliable method for rooting and hardening of in vitro raised microshoots of kiwiberry (Actinidia arguta).