Browsing by Author "Mishra, Santosh Kumar"
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ArticleItem Open Access Atorvastatin restores arsenic-induced vascular dysfunction in rats: Modulation of nitric oxide signaling and inflammatory mediators(Elsevier, 2014) Kesavan, Manickam; Sasindran Sarath, Thengumpallil; Kannan, Kandasamy; Suresh, Subramaniyam; Gupta, Priyanka; Vijayakaran, Karunakaran; Sankar, Palanisamy; Kurade, Nitin Pandurang; Mishra, Santosh Kumar; Sarkar, Souvendra Nath; TANUVASWe evaluated whether atorvastatin, an extensively prescribed statin for reducing the risks of cardiovascular diseases, can reduce the risk of arsenic-induced vascular dysfunction and inflammation in rats and whether the modulation could be linked to improvement in vascular NO signaling. Rats were exposed to sodium arsenite (100 ppm)through drinkingwater for 90 consecutive days. Atorvastatin (10 mg/kgbw, orally)was administered once daily during the last 30 days of arsenic exposure. On the 91st day, blood was collected for measuring serum C-reactive protein. Thoracic aorta was isolated for assessing reactivity to phenylephrine, sodium nitroprusside and acetylcholine; evaluating eNOS and iNOS mRNA expression and measuring NO production,while abdominal aorta was used for ELISA of cytokines, chemokine and vascular cell adhesionmolecules. Histopathologywas done in aortic arches. Arsenic did not alter phenylephrine-elicited contraction. Atorvastatin inhibited Emax of phenylephrine, but it augmented the contractile response in aortic rings from arsenic-exposed animals. Sodium nitroprusside-induced relaxation was not altered with any treatment. However, arsenic reduced acetylcholineinduced relaxation and affected aortic eNOS at the levels of mRNA expression, protein concentration, phosphorylation and NO production. Further, it increased aortic iNOS mRNA expression, iNOS-derived NO synthesis, production of pro-inflammatory mediators (IL-1β, IL-6, MCP-1, VCAM, sICAM) and serum C-reactive protein and aortic vasculopathic lesions. Atorvastatin attenuated these arsenic-mediated functional, biochemical and structural alterations. Results show that atorvastatin has the potential to ameliorate arsenic-induced vascular dysfunction and inflammation by restoring endothelial function with improvement in NO signaling and attenuating production of pro-inflammatory mediators and cell adhesion molecules.ThesisItem Open Access Comparative evaluation of quality of yoghurt prepared from cow, buffalo and goat milk(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 2017) Rima; Mishra, Santosh KumarThe objectives of the present study were to assess and compare the chemical, microbiological and sensory quality of yoghurt made from milk across the species (cow, buffalo and goat) as well as investigating the effect of refrigerated storage on the shelf life of the manufactured yoghurt. Cow milk was found to be salty and sweet in taste with bland and distinctive flavor; buffalo milk was rich, creamy, mild and slightly sweet whereas goat milk was slightly sweeter than cow milk with slight goaty flavor. There was significant difference noticed among all the three species in fat, lactose, total solid, ash, specific gravity, SPC, yeast and mold count(p<0.01), while there was no significant difference observed between protein, acidity and coliform count. After initial screening with different yoghurt starter cultures, NCDC-144 culture was finally selected for optimization. Optimum conditions were found at inoculation rate 3%, temperature 42°C with curd incubation time 6, 5, 5 hours for cow, buffalo and goat milk yoghurt to set, respectively. There was significant difference found in physico-chemical, instrumental texture and colour analysis of the products. Textural properties in terms of firmness and consistency of buffalo milk yoghurt were higher when compared to other species. In SDS-PAGE, extra band was obtained in goat milk yoghurt. During storage, S. thermophilus count showed no significant difference whereas L. bulgaricus count showed significant difference among all the varieties of yoghurt. All the three types of yoghurt were found acceptable after 15 days of storage.ThesisItem Open Access Economics of production and marketing of Paddy in Pratapgarh district of Eastern Uttar Pradesh(ANDUAT, Kumarganj, Ayodhya, 2017) Mishra, Santosh Kumar; Singh, R.A.AddThesisItem Open Access Isolation and characterization of reuterin producing strains of lactobacillus reuteri for their potential use.(NDRI, Karnal, 2012) Mishra, Santosh Kumar; Malik, R.K.ThesisItem Open Access STUDY ON GENDER DIMENSIONS IN RICE BASED FARMING SYSTEM.(Indira Gandhi Krishi Vishwavidyalaya Raipur, 2000) Mishra, Santosh Kumar; Sharma, M.L.; Sharma, P.N.; Rathore, A.L.; Rai, A.L.