Browsing by Author "Kumari Basamma"
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ThesisItem Open Access Conventional and Molecular Approaches in Breeding for High Yield and Disease Resistance in Urdbean (Vigna mungo (L.) Hepper)(UAS Dharwad, 2011) Kumari Basamma; P.M. SalimathA study was conducted to understand the inheritance of resistance to powdery mildew and mungbean yellow mosaic virus (MYMV) in urdbean during 2008 to 2010. TAU-1, a high yielding but powdery mildew susceptible variety was crossed to LBG-17 which is resistant to powdery mildew. The F1, F2 and F3 populations were evaluated along with parents for resistance to powdery mildew under artificial condition. The study indicated that two independent dominant genes together control the resistance reaction in the host plant. Further, attempts were made to identify SSR markers inked to powdery mildew disease following the bulk segregants analysis in F2 population of this cross. Out of 469 SSR primers used for screening parental polymorphism, 32 primers could differentiate the two parents and two were found to be closely linked to powdery mildew disease resistance. Similarly inheritance of resistance to MYMV was studied by crossing TAU-1, (susceptible to MYMV disease) with BDU-4, a resistant genotype. The evaluation of F1, F2 and F3 and parental lines indicated the role of a dominant gene in governing the inheritance of resistance to MYMV. Attempts to identify the marker linked to MYMV did not give satisfactory result with 469 primers used for the study. Since TAU-1 is a high yielding cultivar, the F2 and F3 populations developed using this as one of the parents for inheritance study for diseases, were also evaluated for productivity and its component traits. F2 evaluation revealed high variability and also transgressive segregation. Similarly, evaluation of F3 families further based on selections made in F2 confirmed the superiority of few families in both the populations. Twelve families showing resistance to powdery mildew and high yield and nine F3 families showing resistance to MYMV and high yield were identified. It is suggested to carry forward these promising selections further to develop high yielding and disease resistant lines.ThesisItem Open Access Conventional and Molecular Approaches in Breeding for High Yield and Disease Resistance in Urdbean (Vigna mungo (L.) Hepper)(UAS, Dharwad, 2011) Kumari Basamma; P.M. SalimathA study was conducted to understand the inheritance of resistance to powdery mildew and mungbean yellow mosaic virus (MYMV) in urdbean during 2008 to 2010. TAU-1, a high yielding but powdery mildew susceptible variety was crossed to LBG-17 which is resistant to powdery mildew. The F1, F2 and F3 populations were evaluated along with parents for resistance to powdery mildew under artificial condition. The study indicated that two independent dominant genes together control the resistance reaction in the host plant. Further, attempts were made to identify SSR markers inked to powdery mildew disease following the bulk segregants analysis in F2 population of this cross. Out of 469 SSR primers used for screening parental polymorphism, 32 primers could differentiate the two parents and two were found to be closely linked to powdery mildew disease resistance. Similarly inheritance of resistance to MYMV was studied by crossing TAU-1, (susceptible to MYMV disease) with BDU-4, a resistant genotype. The evaluation of F1, F2 and F3 and parental lines indicated the role of a dominant gene in governing the inheritance of resistance to MYMV. Attempts to identify the marker linked to MYMV did not give satisfactory result with 469 primers used for the study. Since TAU-1 is a high yielding cultivar, the F2 and F3 populations developed using this as one of the parents for inheritance study for diseases, were also evaluated for productivity and its component traits. F2 evaluation revealed high variability and also transgressive segregation. Similarly, evaluation of F3 families further based on selections made in F2 confirmed the superiority of few families in both the populations. Twelve families showing resistance to powdery mildew and high yield and nine F3 families showing resistance to MYMV and high yield were identified. It is suggested to carry forward these promising selections further to develop high yielding and disease resistant lines.ThesisItem Open Access Genetic Variability in selected F2 populations of desi cotton(UAS, Dharwad, 2007) Kumari Basamma; S.T.KajjidoniAn investigation was carried out during kharif 2005 to assess the variability generated by hybridization and to study the association pattern among the component traits of seed cotton yield. Five crosses involving seven genotypes viz., DD-8NLE, RDC-88, 9749, Blach- 1, MDL 2582, MDL 2601 and DLSA-17 were selected based on their F1 performance for components of seed cotton yield and fibre equality traits. The segregating populations thus obtained were evaluated for eight quantitative traits on 100 randomly selected plants in each cross. There was increase in mean and variance in F2 populations for majority of traits except for seed index and lint index as compared to the parents. A wide range was observed for boll number per plant, lint yield per plant, boll weight, ginning out turn and seed cotton yield per plant. The GCV, heritability and GAM estimates were higher in F2s for seed cotton yield per plant, number of bolls per plant, lint yield per plant. High heritability coupled with high genetic advances were estimated for boll number, lint yield and seed cotton yield per plant in all the segregating populations. Seed cotton yield recorded significant positive correlation with number of bolls, lint yield in all the segregating populations and with boll weight in three segregating populations indicating that more emphasis should be given for number of bolls, boll weight and lint yield for improvement in desi cotton yield through selection. Path coefficient analysis of seed cotton yield across the different populations revealed the maximum direct contribution of number of bolls per plant, boll weight and lint yield and further the same trait had maximum indirect contribution through lint yield per plant confirming these traits as important seed cotton yield attribute. More number of transgressive segregants were recorded for boll weight followed by lint index and seed index traits.