Browsing by Author "Kulkarni, Shripad"
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ThesisItem Open Access Isolation, Characterization, Screening for Bioefficacy and Mass Multiplication of Fluorescent Pseudomonads(University of Agricultural Science, Dharwad, 2016-08) Lamani, Beeresh; Kulkarni, ShripadA random survey conducted during kharif 2015 in Belagavi, Dharwad and Haveri districts of northern Karnataka revealed that black rot of cabbage is a major disease in all the districts surveyed. Maximum disease index of 23.36 per cent was noticed in Belagavi district followed by Dharwad (20.17 %) and the least disease index was observed in Haveri (18.65 %). Fluorescent pseudomonads were observed in all districts surveyed and eight fluorescent pseudomonads were isolated and identified based on their morphological characters, physiological and biochemical tests. Isolates were circular and creamy white in colour. They were showed positive for oxidase, catalase, gelatin liquefaction and fluorescence tests. In vitro studies revealed that isolate KKL-FP showed maximum inhibition of Xanthomonas campestris pv. campestris by producing 18.33 mm inhibition zone. In the pot culture experiment, fluorescent pseudomonad (KKL- FP) was tested for its bioefficacy against black rot of cabbage. In seed treatment method, 10 g/kg and in seedling dip method, 20 g/ l produced maximum number of leaves and head weight per plant. Seed treatment with hot water and KKL- FP (10 g/ kg) + seedling dip (20 g/l) + 2 sprays (10 g/l) was the best treatment to reduce black rot incidence in integrated disease management. Fungicides such as carbendazim, copper hydroxide, copper oxychloride and hexaconazole were compatible at 0.1 and 0.2 per cent concentrations. Bactericides such as Bacterinashak, Bacterinol and Streptocycline were non compatible at 100, 250, 500 and 750 ppm concentrations except Bronip was compatible up to 750 ppm concentrations. The growth of fluorescent pseudomonad (KKL- FP) strain was good in King’s B broth. Temperature of 25-30 0C and 7-8 pH favoured the maximum growth and multiplication. Talc as a carrier material maintained viable cfu count (22.33 × 107 cfu/g) even up to six months of storage under normal condition.ThesisItem Open Access Studies on Epidemiology and Management of Cucumber Powdery Mildew(University of Agricultural Science, Dharwad, 2017-08) Parameshwar Naik H.; Kulkarni, ShripadThe present investigation involving roving survey in 4 districts, epidemiological studies, in vitro evaluations, field screening of 23 genotypes, molecular characterization of pathogen and management was carried out at the University of Agricultural Sciences Dharwad during the year 2016-17. In survey maximum disease severity was recorded in Dharwad (33.06 PDI) and least in Haveri (27.21 PDI). Under protected cultivation maximum disease severity (64.01 PDI) was recorded in Dharwad and least was recorded in Haveri (40.07 PDI). The rDNA-ITS sequencing of E. cichoracearum indicated that Dharwad isolate had 96 and 94 per cent homology with Berkely isolate and Australian isolate. Highest conidial germination (48.52 %) was observed at 250 C and 85 per cent relative humidity. Sowing during second fortnight of August recorded maximum severity and maximum temperature showed positive correlation whereas, rainfall and number of rainy days were significantly negatively correlated. Azoxystrobin showed maximum conidial germination inhibition (94.51 %) among the combiproducts, tebuconazole 50 % + trifloxystrobin 25 % resulted in maximum inhibition (87.78 %). In bioagents tested maximum inhibition observed with Bacillus subtilis (55.74 %), Leaf extract of Reynotriu sachalensis resulted in 100 per cent inhibition. 15, five and three genotypes were found to be moderately resistant, moderately susceptible and highly susceptible respectively. Field evaluation of fungicides revealed that two sprays of tebuconazole 50% + trifloxystrobin 25% with least PDI (6.73) and yield up to 11.42 t/ha with B:C ratio of 1:3.60 which was significantly superior followed by two sprays of Azoxystrobin (14.66 PDI) with an yield of 10.16 t/ha.