Browsing by Author "Kaur, Manpreet"
Now showing 1 - 7 of 7
Results Per Page
Sort Options
ThesisItem Open Access Genetical studies in parthenocarpic cucumber (Cucumis sativus L.)(palampur, 2022-05-21) Kaur, Manpreet; Sharma, ParveenThe present investigation entitled “Genetical studies in parthenocarpic cucumber (Cucumis sativus L.)” was carried out at the Experimental Farm, Department of Vegetable Science and Floriculture, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur to gather information on combining ability, gene action and magnitude of heterosis in parthenocarpic cucumber. Eight parental lines were used to develop F1 hybrids through half diallel mating design (excluding reciprocals). All parental lines and hybrids were evaluated along with standard check Kian in RBD with three replications under modified naturally ventilated polyhouses during spring-summer seasons of 2020 and 2021. Genetic diversity of eight parental lines was assessed using SSR markers to identify the diverse parents. PCA revealed 54.04 per cent variation as justified by first three principal components and maximum genetic variation (21.10%) was contributed by PC1. Eight cucumber genotypes were grouped into two clusters and both the clusters were divided into 2 sub clusters (NTSYS software) and DARwin software exhibited three clusters out of which cluster II was further divided into two sub clusters and was also confirmed by population structure. The analysis of variance indicated significant differences among genotypes (G) for fruit yield and other component traits in all the environments. Significant differences for environment (E) and G × E interaction for most of the traits indicated the role of environment on the performance of genotypes/crosses. Combined analysis of variance also indicated that mean squares due to parents, hybrids and parents vs. hybrids were significant for most of the traits. Variances due to GCA, SCA and their interaction with environment were significant for most of the traits, indicating the importance of testing parents as well as hybrids across environments. The top cross combinations identified on the basis per se performance were DDPCG1 × DDPCG2, DDPCG1 × DDPCG5, DDPCG1 × PPC-2 and DDPCG1 × PPC-3 for fruit yield and its component traits under protected environment. Parents DDPCG1, DDPCG4, PPC-2 and DDPCG5 were observed to be good general combiners for earliness, fruit yield and its component traits. Cross combinations viz., DDPCG4 × DDPCG5, DDPCG1 × PPC-2, DDPCG1 × DDPCG5, PK-1 × PPC-3 and DDPCG1 × DDPCW1 were the most promising crosses on the basis of SCA effects for yield and its related traits. For most of the traits, there was preponderance of non-additive gene action which reaffirms the importance of hybrids in cucumber. A wide variation in magnitude and direction of heterosis was noticed for majority of the traits with maximum manifestation for number of fruits per plant and marketable yield per plant. Hybrids DDPCG1 × DDPCG2, DDPCG1 × DDPCG5, DDPCG1 × PPC-2, DDPCG1 × PK-1 and DDPCG1 × DDPCW1 were the most promising crosses with significant economic heterosis for yield and yield related traits. Based on per se performance, SCA effects and heterosis, DDPCG1 × DDPCG2, DDPCG1 × DDPCG5, DDPCG1 × PPC-2, DDPCG1 × PK-1 and DDPCG1 × DDPCW1 were the most promising hybrid combinations for protected environmentThesisItem Restricted In Vitro Effects of Anthelmintics on Histopathology of Some Nematodes of Sheep / GOAT(Department of Zoology College of Basic sciecne And Humanities PAU, Ludhiana, 1992) Kaur, Manpreet; Sood, M. L.ThesisItem Open Access Microarthropod fauna of cultivated soil(CSKHPKV, Palampur, 2004) Kaur, Manpreet; Chandel, Y.S.ThesisItem Open Access Molecular Characterization of Structural Classical Swine Fever Virus (CSFV)(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 2020) Kaur, Manpreet; Singh, Niraj KumarClassical swine fever (CSF) is a highly contagious disease of swine. CSF virus (CSFV), the causative agent of CSF, is a member of the genus Pestivirus, which belongs to the family Flaviviridae. In current study, molecular characterization and codon usage pattern analysis of C and Erns gene of field isolates of CSFV was carried out. A total 17 tissue/blood samples were collected from dead piglet suspected for CSF. One sample was found positive for CSFV in PCR based diagnosis. Further C and Erns gene of positive sample was amplified, cloned and sequenced. Sequencing results indicated that Punjab isolate virus belonged to genotype 1.1. Codon usage analysis of CSFV was carried out by retrieving 111 C and Erns gene sequences and gene sequence obtained in this study. Codon usage analysis indicated that both mutational and natural selection pressure influencing on CSFV.ThesisItem Open Access NUTRITIONAL,BIOCHEMICAL AND CULTIVATION STUDIES OF REISHI MUSHROOM (Ganoderma lucidum)(Punjab Agricultural University, Ludhiana, 2007) Kaur, Manpreet; Khanna, P.K.The conditions for biomass production of two strains of Ganoderma lucidum GLS and GLM in liquid medium were optimized. Mushroom complete medium, 25°C temperature, 12 hr light exposure/day and supplementation with malt extract and ammonium chloride were the four optimized parameters for maximum biomass production. Proximate chemical composition of mycelium of two strains was determined and it contained 27.5-30.2 per cent carbohydrates, 10.5-12.2 per cent protein, 5.5-6.0 percent fat, 4.0-4.9 per cent ash and 46.7-52.5 per cent fibres. The strains were evaluated for production of lignocellulolytic enzymes. Maximum Endo β-D 1,4 glucanase (CMCase) and xylanase activity was observed in GLS strain whereas maximum β-glucosidase (Cellobiase) activity was observed in GLM strain. Growth behaviour of mycelium on solid substrate i.e. wheat straw and saw dust was studied. Maximum spawn run was observed on 20% wheat bran supplemented wheat straw with 1.75 mm/day mycelial extension rate in GLS strain. It took 49 days for impregnation for substrate with mycelium of G. lucidum.ThesisItem Open Access Studies on polymorphism of CXCR1 gene and its association with subclinical mastitis in Indian water buffalo(GADVASU, Ludhiana, 2015-09-24) Kaur, Manpreet; RamneekSub clinical mastitis (SCM) in buffalo is considered as multi-factorial disease, which leads to huge economic losses by reducing the quality and quantity of the milk. Although bacteria are considered to be the main causative agent for mastitis, farm management practices, genetic make-up of the animal are found to be associated with the disease. So the present study aimed at identification of SNPs in the bovine CXCR1 through PCR-SSCP, DNA sequencing and its association if any with mastitis tolerance/succeptibility in Indian water buffalo... Animal wise the prevalence of sub clinical mastitis was found to be (27. %), and 7.9%, 9.9%, 2.9% quarters were found to be affected with latent mastitis, specific mastitis and nonspecific mastitis respectively . On the basis of individual quarter, the highest prevalence was in left hind (33.5%), followed by right hind (29.7%) and right front (22.2%) and the least prevalence in left front (14.6%). The microorganisms isolated from sub clinical mastitis constituted Staphylococci (39%), Streptococci (31%), Corynebacterium (25%) and E.coli. (5%).PCR-SSCP when used to explore polymorphism in partial exon 2 of CXCR1 gene, the 311bp fragment of this gene was found to be monomorphic in all the DNA samples screened. SSCP analysis of the exonic amplicons and DNA sequence analysis of CXCR1 gene exonic sequences (using one set of PCR primers) did not reveal any polymorphism in this region of CXCR1 gene in diseased or healthy buffaloes. Farm management associated determinants (type of feed, type of floor, education of the owner, feeding after milking, type of labor) when studied in association with sub clinical mastitis, education of the owner , type of labor and feeding after milking were found to be significantly associated with disease occurrence (p<0.05). While looking at the animal determinants in association with sub clinical mastitis in the present investigation, buffaloes with higher parity showed more risk of developing sub clinical mastitis. So the present investigation helped to understand the various risk factors for the occurrence of sub clinical mastitis in buffaloes in Punjab.ThesisItem Open Access STUDY ON QUALITY ATTRIBUTES OF COMPOSITE MEAT CHOCOLATE(Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, 2017) Kaur, Manpreet; Sunil KumarComposite meat chocolate, a novel product with enhanced nutritional quality was developed by using animal fat as vegetable fat replacer and then replacing a part of milk chocolate with dehydrated ground chicken meat. Calcium lactate as calcium supplement was added to the developed composite meat chocolate. In first experiment four samples of milk chocolate were prepared by replacing a part of cocoa butter with 0, 10, 20 and 30% of animal fat constituting control and three treatments in ascending order, to improve its textural quality during storage in tropical regions. With the increase in the amount of animal fat replaced, a significant (P<0.05) decrease in sensory attributes and a significant (P<0.05) increase in cholesterol content was observed in third and fourth treatment. However the pH and proximate parameters showed non-significant (P>0.05) difference. Further In second experiment, the second treatment selected from first experiment was taken as control and again four samples were prepared by replacing a part of the milk chocolate containing 10% animal fat with 0, 10, 20 , 30 % dehydrated ground chicken meat. Moisture and protein content showed significant (P<0.05) increase with increase in the amount of meat replaced. While as pH and fat content decreased significantly (P<0.05). A significant increase in cholesterol and decrease in antioxidant activity was observed in second, third and fourth treatment. Sensory scores for third and fourth treatments decreased significantly (P<0.05). In third experiment calcium lactate was added to composite meat chocolate with optimized animal fat and dehydrated chicken meat @ 0.5, 1.0 and 1.5 % level. There was a significant (P<0.05) drop in pH and sensory scores at 1.5% level. However ash content increased significantly with increase in quantity of calcium lactate. Further in experiment fourth, the calcium fortified optimized composite meat chocolate was added with 1, 2 and 3% of blueberry and raspberry extracts and ten samples were prepared. The samples were then stored at room temperature (25±5 C) and refrigeration (4±1 C). Antioxidant activity of samples with fruit extracts was significantly higher compared to composite meat chocolate with no calcium lactate. pH significantly (P<0.05) dropped with time of storage in control and not in treated samples. FFA and TBARS values for samples stored at both refrigerated and room temperature increased significantly after 6 months of storage in all samples however in samples containing highest level of fruit extracts showed non-significant increase. Sensory scores of samples stored at refrigeration as well as room temperature showed significant decrease after 6 months of storage. Microbial count of all samples was within acceptable level even after 6 months of storage.