Browsing by Author "Karuppannan, AK"
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ArticleItem Open Access Emergence of Porcine Circovirus 2 Associated Reproductive Failure in Southern India(2014) Karuppannan, AK; Ramesh, A; Reddy, YK, et al.,; TANUVASIncidence of unusually high numbers of stillbirths was observed at a piggery unit at the Veterinary University research farm in Tamil Nadu State of India. Systematic examination of the tissue from stillborn piglets led to the identification of presence of Porcine circovirus 2 (PCV2). Detailed analysis utilizing electron microscopy, polymerase chain reaction and sequencing confirmed the presence of PCV2 in the tissue of affected piglets. Histopathology analysis of the affected piglet tissue showed lymphoid cell depletion of lymphnodes, spleen and infiltration of liver, kidney, myocardium, etc. Retrospective examination of the morbidity and mortality history in the farm revealed high mortality in young and weanling piglets suggestive of PCV2 infection-induced diseases. This is the first report of emergence of major disease incidence in farmed swine due to PCV2 infection in India.ArticleItem Open Access Rapid and Sensitive Faecal based PCR Assays for the Detection of Parvoviruses in Laboratory Rats(2020-08) Srinivasan, MR; Vijay, K; Ramesh, S; Karuppannan, AK; Krishnamohan Reddy, Y; TANUVASHealth status of laboratory rats indicates its suitability for the researches. They are prone to develop viral infections of subclinical nature caused by parvoviruses, which affects the research results adversely; Hence Implementation of health monitoring protocol is essential at the level of breeding colony itself. This study was intended to develop rapid and sensitive fecal-PCR assays to detect infections of multiple species of Parvoviruses affecting rats namely, Rat Minute Virus, Toolan’s Parvo Virus, Rat Parvo Virus and Kilham’s Rat Virus as an alternate approach to serology based health monitoring in a lab animal breeding unit. All the primers detected only in the presence of the respective templates. PCR assays of RMV and TPV consistently amplified as little as 40 fg and that of RPV and KRV consistently amplified as little as 4 fg of plasmid DNA. Specificity and sensitivity assays indicate that the PCR assays may be useful as diagnostic tools for rapid detection of natural acute viral infections. Further analysis of the primers in the positive laboratory animal colony is essential.