Browsing by Author "Karthiayini, K."
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ArticleItem Open Access Effect Of Feed Nitrogen On the Ammonia Production in the Rumen(Indian Veterinary Association, 2002-06) Karthiayini, K.; Nayeem, Mohammed; TANUVASThe protein requirements of the ruminants are mainly met by the microbial protein and the non-degraded protein which escapes the rumen fermentation. The microbial proteins are synthesised using the carbon, and energy coming from the fermentation of carbohydrated and nitrogen from protein and non-protein nitrogenous (NPN) substances in the feed.ThesisItem Open Access EXPRESSION PROFILE OF INSULIN-LIKE GROWTH FACTOR 1 (IGF1) GENE AND ITS RECEPTOR, IGFR1 IN GRANULOSA CELLS OF GOATS(COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR, 2018-01-31) BIJNA., M; Karthiayini, K.The experiment was conducted to know the expression profile of IGF1 and its biological receptor IGFR1 with increase in size of goat ovarian follicles and also the histology and micrometry of ovarian follicles. For the study, ovaries of healthy Malabari goats collected from Meat Technology Unit, KVASU were used. Granulosa cells from pooled samples (n=6) of follicular fluid from medium (35mm) and large (>5mm) follicles were analysed by Real-time quantitative polymerase chain reaction for assessing the expression pattern of IGF1 and IGFR1 genes in medium and large follicles. There was significant difference (p<0.05) in the expression levels of IGF1 and IGFR1 genes between two groups of follicles. The expression of IGF1 and IGFR1 mRNA in granulosa cells of caprine follicles indicated that these genes might play a role in granulosa cell proliferation and steroidogenic activity of granulosa cells during transition from medium to large follicles. However, for the conclusive assessment of the role of IGF1 in follicular development, the level of IGF1 protein should be quantified and correlated with the mRNA expression of IGF1. The dimensions of ovary like breadth, weight and thickness of right and left ovaries of Malabari breed of goats were not significantly different while the length of right ovary was significantly more than that of the left. The mean number of medium and large follicles observed in right and left ovaries were not significantly different. There was no significant difference in the mean micrometric values of medium follicles (3-5mm) and large follicles studied. However a non-significant increase in thickness of granulosa layer was noticed in the large follicles compared to medium sized follicles.