Browsing by Author "Kalaivanan, M"
Now showing 1 - 9 of 9
Results Per Page
Sort Options
ArticleItem Open Access Development of multiplex PCR for the simultaneous detection of Trypanosoma evansi, Babesia canis and Hepatozoan canis in dogs(2019) Kalaivanan, M; Saravanan, S; TANUVASSince co-infections with canine tick borne pathogens are common there is greater risk for multiple infections which may necessitate different clinical management strategies. In the presnet study a multiplex polymerase chain reaction (mPCR) by using the species specific primers was developed for the simultaneous detection of the most pathogenic haemoparasites, Trypanosoma evansi, Babesia canis and Hepatozoan canis from the blood of naturally infected dogs so as to enable early diagnosis and treatment.ArticleItem Open Access Development of PCR to identify the prevalence of Trypanosoma evansi in dogs from South India.(2019-06) Kalaivanan, M; Saravanan, S; Palanivel, KM; Ponnudurai, G; TANUVASDogs are continuously exposed to a spectrum of haemoparasitic diseases and the information on the distribution and epidemiology of trypanosomosis in India is still under reported. Dogs brought to Teaching Veterinary Clinical Campus, Veterinary College and Research Institute, Namakkal of Tamil Nadu were screened for the haemoparasite, T. evansi by polymerase chain reaction. The PCR was developed for detection of Trypanosoma evansi in dogs targeting Beta tubulin mRNA gene at 997 bp, which revealed a positivity of 1.33 per cent. The target gene of T. evansi identified was found to possess nearly a complete homology (99.6-99.8%) with that in China and India. The parasite could not be detected by microscopic examination of the Giemsa stained peripheral blood smears. High prevalence was noticed in dogs of less than 1 year age group (2.94%), males (1.85%) and non-descript dogs (2.94%).ArticleItem Open Access EFFICIENCY OF PCR IN THE DETECTION OF HAEMOPARASITIC INFECTIONS IN BUFFY COAT, LYMPH NODE AND BLOOD OF DOGS(2018) Kalaivanan, M; Saravanan, S; TANUVASBlood, buffy coat and lymph node biopsy samples were collected from dogs with clinical signs related to haemoparasites and screened for the haemoparasites E. canis, Babesia spp., H. canis and T. evansi by polymerase chain reaction. A high sensitivity for the haemoparasites by PCR on blood than any other type of samples was identified.ArticleItem Open Access Identification of Babesia gibsoni in dogs from Namakkal region by polymerase chain reaction(2021) Kalaivanan, M; Saravanan, S; Ponnudurai, G; TANUVASDogs brought to Teaching Veterinary Clinical Complex, Veterinary College and Research Institute, Namakkal were screened for Babesia gibsoni by conventional microscopic examination and polymerase chain reaction. The parasite could not be detected by microscopy, however, polymerase chain reaction targeting 18S rRNA gene of B. gibsoni revealed a positivity of 2.0 per cent. The amplicon of B. gibsoni had complete homology with the isolates from India and other countries. Male, adult dogs of nondescript breeds were found to be highly susceptible for babesiosis. The PCR was found to be useful in the epemiological survey of B. gibsoni in dogs.ArticleItem Open Access Identification of canine haemoparasites in transmitting tick vectors by polymerase chain reaction(2020) Kalaivanan, M; Saravanan, S; Palanivel, KM; TANUVASThe objective of this study was to identify the canine haemoparasites in ticks infesting the dogs in the local region. Ticks collected from dogs with clinical signs related to haemoparasites and screened for the haemoparasites Ehrlichia canis, Babesia spp., Hepatozoan canis and Trypanosoma evansi by polymerase chain reaction. The H. canis (9.67%) and E. canis (6.45%) were the haemoparasites detected by PCR. The transmitting tick vector was identified as Rhipicephalus sanguineus.ArticleItem Open Access IDENTIFICATION OF EHRLICHIA CANIS BY PCR WITH PHYLOGENETIC ANALYSIS IN DOGS FROM SOUTH INDIA(2020-03) Kalaivanan, M; Saravanan, S; Palanivel, KM; Ponnudurai, G; TANUVASCanine monocytic ehrlichiosis (CME) or canine hemorrhagic fever is a potentially fatal tick-borne disease in tropical countries associated with its deleterious effect on the health of dogs. Clinically affected (n=130) and apparently healthy dogs (n=20) for the purpose of deworming or vaccination brought to Teaching Veterinary Clinical Complex (TVCC), Veterinary College and Research Institute (VC &RI), Namakkal were screened for E. canis by microscopic examination and polymerase chain reaction assay. The PCR assay revealed a positivity of 8.0 percent samples for E. canis targeting Vir-B9 gene and generating an amplicon of 380 bp. However, conventional microscopic blood smear examination failed to detect E. canis owing to the low parasitaemia. The sequence of E. canis had a 100.0 per cent homology with the E. canis of dogs in India and USA. The tick vector from infected dogs was identified as Rhipicephalus sanguineus. Dogs below1 year age group, females and pure breeds were found to be more affected.ArticleItem Open Access IDENTIFICATION OF EHRLICHIA CANIS BY PCR WITH PHYLOGENETIC ANALYSIS IN DOGS FROM SOUTH INDIA(2020-03) Kalaivanan, M; Saravanan, S; Palanivel, KM; Ponnudurai, G; TANUVASCanine monocytic ehrlichiosis (CME) or canine hemorrhagic fever is a potentially fatal tick-borne disease in tropical countries associated with its deleterious effect on the health of dogs. Clinically affected (n=130) and apparently healthy dogs (n=20) for the purpose of deworming or vaccination brought to Teaching Veterinary Clinical Complex (TVCC), Veterinary College and Research Institute (VC &RI), Namakkal were screened for E. canis by microscopic examination and polymerase chain reaction assay. The PCR assay revealed a positivity of 8.0 percent samples for E. canis targeting Vir-B9 gene and generating an amplicon of 380 bp. However, conventional microscopic blood smear examination failed to detect E. canis owing to the low parasitaemia. The sequence of E. canis had a 100.0 per cent homology with the E. canis of dogs in India and USA. The tick vector from infected dogs was identified as Rhipicephalus sanguineus. Dogs below1 year age group, females and pure breeds were found to be more affected.ArticleItem Open Access Spectrum of Signs Associated with Haemoparasitic Infections in Dogs(2020-01) Kalaivanan, M; Saravanan, S; Palanivel, KM, et al.; TANUVASDogs are continuously exposed to haemoparasitic infections. However most of the cases go unnoticed due to the inapparent or subclinical infections caused by the parasites. The spectrum of signs in dogs positive for haemoparasitic infections by polymerase chain reaction were identi ed and the positivity was correlated with the health status of dogs. Anorexia (74.35%) and lymphadenopathy (58.97%) were found to be the predominant clinical signs in positive cases. The prevalence of both B. canis and H. canis was found to be more in dogs with icterus (20.2%), where as the prevalence of B. gibsoni and T. evansi was found to be more in dogs with tick infestation (6.06% and 3.03, respectively), and E. canis in dogs with epistaxis (25.0%). The prevalence was found to be more in dogs presented with non-speci c signs (48.71%) than in other groups.ArticleItem Open Access Spectrum of Signs Associated with Haemoparasitic Infections in Dogs(2020-01) Kalaivanan, M; Saravanan, S; Palanivel, KM; Ponnudurai, G; TANUVASDogs are continuously exposed to haemoparasitic infections. However most of the cases go unnoticed due to the inapparent or subclinical infections caused by the parasites. The spectrum of signs in dogs positive for haemoparasitic infections by polymerase chain reaction were identi ed and the positivity was correlated with the health status of dogs. Anorexia (74.35%) and lymphadenopathy (58.97%) were found to be the predominant clinical signs in positive cases. The prevalence of both B. canis and H. canis was found to be more in dogs with icterus (20.2%), where as the prevalence of B. gibsoni and T. evansi was found to be more in dogs with tick infestation (6.06% and 3.03, respectively), and E. canis in dogs with epistaxis (25.0%). The prevalence was found to be more in dogs presented with non-specic signs (48.71%) than in other groups.