Browsing by Author "KUMAR, VIVEK"
Now showing 1 - 1 of 1
Results Per Page
Sort Options
ThesisItem Open Access GENETIC ARCHITECTURE OF BLACK BENGAL GOAT IN CONTEXT TO ITS FECUNDITY(Birsa Agricultural University, Kanke, Ranchi, Jharkhand, 2013) KUMAR, VIVEK; Singh, L. B.The present study was carried out on Black Bengal goats which is prolific meat purpose breed of India. Animals were maintained at Instructional Small Ruminant Farm, Ranchi Veterinary College, B.A.U., Ranchi. In this investigation, goat population was screened for polymorphism of FecB gene. A study of association of different variants with different reproductive traits and absolute body weights at different stages of growth was also done. With the primers having forward and reverse base sequence as 5'-CCAGAGGACAATAGCAAAGCAAA-3' and 5'-CAAGATGTTTTC ATGCCTCATCAACAGGTC-3', PCR products obtained were subjected to polyacrylamide gel electrophoresis for the detection of Single Strand Conformational Polymorphism (SSCP). As a result, three different SSCP variants were found which were designated as AA, AB and BB. The highest genotype frequency was observed for AB (0.38), which was followed by BB (0.33) and AA (0.29). Least-square analysis of variance showed significant (P<0.01) effect of genotype on litter size at birth. The result revealed that highest litter size at birth was found to be 02.06±00.08 for genotype BB, which differed significantly (P<0.01) from that of genotype AA (01.14±00.08) and genotype AB (01.27±00.07). Further, it was found that genotype had significant (P<0.01) effect on litter size at weaning. The highest litter size at weaning was found to be 01.81±00.06 for genotype BB, which differed significantly from that of genotype AA (01.00±00.08) and genotype AB (01.12±00.06). There was non-significant effect of genotype on age at first kidding, service period, kidding interval and gestation period. Moreover, there is significant (P<0.01) effect of genotype on body weight at birth. The least square mean of body weights at birth for genotype BB (01.17±00.03 kg) was significantly lower than that of genotype AA (01.43±00.03 kg) and genotype AB (01.36±00.02 kg). The different genotype were non-significantly associated with absolute body weight at 4-week, 8-week, 12-week, 24-week, 36-week and 48-week. Nucleotide sequences of the allelic variants were also analyzed. The DNA sequence showing polymorphism observed were used to identify SNPs. Principal SNP was found at 78 position of gene sequence, which shows transition of Adenine (AA genotype) to Guanine (BB genotype). On the basis of this investigation, following conclusion were observed: 1. The PCR-SSCP analysis of FecB gene revealed the polymorphic pattern of genotypes in Black Bengal Goat. It suggests that flow of FecB mutation was present in Black Bengal Goat. 2. There was significant association of different polymorphic variants of FecB gene with the litter size at birth and at weaning and body weight at birth.