Browsing by Author "KANUPRIYA"
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ThesisItem Open Access ANALYSIS OF GENETIC DIVERSITY IN POMEGRANATE USING SRAP (Sequence Related Amplified Polymorphism) MARKER(COLLEGE OF HORTICULTURE, VENKATARAMANNAGUDEM, WEST GODAVARI – 534 101 Dr. Y. S. R. HORTICULTURAL UNIVERSITY) SHWETHA, H. K.; KANUPRIYAPomegranate (Punica granatum L.), is one of the most important fruit crop of arid and semiarid regions. In the present study, Sequence-related amplified polymorphism (SRAP) was used to assess the genetic diversity of 114 pomegranate genotypes maintained in the field gene bank at ICAR-Indian Institute of Horticultural Research, Hesaraghatta, Bangalore. The DNA from these genotypes was isolated using CTAB method. Totally 40 SRAP combinations were used for screening, 10 primer combinations showed polymorphism. The selected primer combinations are used for polymerase chain reaction along with 114 pomegranate genotypes. The PCR products were then run on agarose gel electrophoresis the bands were observed under UV transilluminator. All intense, reliable, and clearly distinguished SRAP products were scored as presence (1) and absence (0) to analyse the maximum polymorphism producing primer combinations. The number of alleles per locus ranged from 1.66 to 4.00 with an average of 2.03. The average polymorphic information content (PIC) value was 0.24.The expected heterozygosity varied from 0.215 to 0.445 with an average of 0.306. The cluster analysis was performed using DARWIN version 6.0 software. The dissimilarity coefficients ranged from 0 to 1. All the 114 genotypes were grouped into three major clusters. There was no clear grouping based on origin. The analysis of molecular variance (AMOVA) indicated no significant genetic variation (p=0.31) between pomegranate genotypes from different geographical locations. Overall genetic variation among the population groups was low, while 99% of variation was due to within group differences. These results confirmed that SRAP markers could be powerful tools and an effective marker system for determining the genetic diversity and population genetic structure of the pomegranate.ThesisItem Restricted EFFECT OF GROWTH PROMOTERS ON AGARICUS BISPORUS(PUNJAB AGRICULTURAL UNIVERSITY, LUDHIANA, 2000) KANUPRIYA; JOHL, P. P.09062017_0003_0007_Abstract.pdfThesisItem Open Access INTERGENERIC HYBRIDIZATION AND PROGENY EVALUATION IN PAPAYA (Caricapapaya L.)(University of Horticultural Sciences, Bagalkot. (College of Horticulture, Bengaluru)., 2015-06) YANTHAN, LICHAMO J.; C., VASUGI; T. R., GURUPRASAD; M., MAHADEVAMMA; K. S., SHIVASHANKAR; KANUPRIYAStudies on “Intergeneric hybridization and progeny evaluation in Papaya (C.papaya L.)” was carried out in the Division of Fruit crops, Indian Institute of Horticultural Research, Bengaluru during 2014-2015. Two systematic experiments were carried out; to overcome the crossability barriers between Carica papaya hybrid varieties ArkaSurya and ArkaPrabhath with Vasconcelleacundinamarcensis and to evaluate the advanced intergeneric hybrid progenies (38) of the cross Arka Surya x V. cauliflora for PRSV tolerance. In overcoming the intergeneric crossability barriers,among the nutrients tried, the combined effect of Sucrose 5 per cent + Boric acid 1.0 per cent + Calcium Nitrate 1.0 per cent + Magnesium Sulphate 1.0 per cent + Potassium Nitrate 1.0 per centresulted in significantly highest mean value for fruit set (58.50%), number of fruits harvested (31.00), number of fruits recovered with fertile seeds (5.0) and number of seeds recovered (282.00). In the evaluation of advanced intergeneric hybrid progenies (38) of the combination Arka Surya X V. cauliflorafor PRSV tolerance,significant differences were observed among theprogenies for vegetative characters such as plant height, stem girth and canopy spread and fruit charactersviz., fruit weight, fruit length, pulp thickness,number of fruits, yield per tree, total soluble solids, carotenoids and lycopene. Traits like fruit weight, fruit width, fruit volume were found to be significantly correlated both phenotypically and genotypically. On the basis of PRSV tolerance, fruit and yield parameters, seven progenies viz., R5P16, R6P16, R7P16, R14P7, R17P16, R19P1and R35P10 were identified for further advancement.