Browsing by Author "Josy, Anjana"
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ArticleItem Open Access Antimicrobial Resistance Pattern of Klebsiella spp. Isolated from Animal Handlers(New Delhi Publishers, 2018-08) Josy, Anjana; Gunaseelan, L; Porteen, K; Sriram, P; Anusha, SA; Balaji, P; TANUVASKlebsiella spp. are ubiquitous members of Enterobacteriaceae that inhabits the gastrointestinal tract of humans and animals. Opportunistic pathogens like Klebsiella pneumonia and K.oxytoca are capable of causing nosocomial infections of surgical wounds, urinary tract, biliary tract, lower respiratory tract etc. and can also harbour genes coding for antimicrobial resistance (AMR). For the present study, 480 hand and nasal swabs were collected from 120 animal handlers in an attempt to isolate Klebsiella spp. Out of 118 presumptive colonies of Klebsiella spp. obtained by conventional culture method, 67 were confirmed as the same by biochemical tests and polymerase chain reaction that targeted gyrA gene. AMR pattern of those confirmed isolates done by disc diffusion method revealed maximum resistance to penicillin (100%) followed by enrofloxacin (94.73%) and maximum susceptibility to gentamicin (84.21%) followed by tetracycline (68.42%).ThesisItem Restricted DETERMINATION OF ANTIMICROBIAL RESISTANCE PATTERN OF MAJOR BACTERIAL PATHOGENS OF PUBLIC HEALTH SIGNIFICANCE(2018) Josy, Anjana; Gunaseelan, L; Porteen, K; Sriram, P; TANUVASThere is increasing concern about the rapid emergence and spread of antibiotic resistant bacteria and antimicrobial resistance has been considered as the most vital health issue of 21st century by WHO. The close contact between animals and people offers favourable conditions for bacterial transmission and this initial study attempts to isolate selected pathogens of common interest in man and animals from hand swabs and nasal swabs of animal handlers, viz., Staphylococcus spp., Streptococcus spp., Klebsiella spp. and Pseudomonas spp. and to determine their antimicrobial resistance pattern both phenotypically and genotypically.