Browsing by Author "JAWAHAR LAL KATARA"
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ThesisItem Open Access Molecular typing of native Bacillus thuringiensis (Bt) isolates from diverse habitats in India and isolation and characterization of cry2-type genes from these isolates(IARI, National Research Centre on Plant Biotechnology, 2010) JAWAHAR LAL KATARA; N.K. SinghBacillus thuringiensis is a bacterium of great agronomic and scientific interest. The subspecies of this bacterium colonize and kill a large variety of host insects and even nematodes, but each strain does so with a high degree of specificity. Molecular typing and diversity analysis of B. thuringiensis has enormous importance for discrimination of strains from different sources. In this study, 113 native B. thuringiensis isolates collected from diverse habitats and locations in India and 27 B. thuringiensis reference strains obtained from the Bacillus Genetic Stock Centre (BGSC), Ohio State University, USA were analyzed for molecular typing. Genotypic data of 140 B. thuringiensis isolates and type strains was generated by using REP-PCR and ERIC-PCR primers and grouped into 4 clusters using Unweighted Pair Group Method with Arithmetic Mean (UPGMA) analysis using NTSYSpc2.2. The four groups have isolates from diverse origins. No group was found representing any specific origin or location. The observed patterns of REP-PCR and ERIC-PCR pattern were discriminatory enough to reveal differences in the bacterial strains. Our results indicate the resolution power and marker index of the ERICPCR (RP 9.39, MI 6.34) to be higher than that of the REP-PCR (RP 6.20, MI 4.48). The REP-PCR and ERIC-PCR markers were found useful for discrimination of B. thuringiensis strains, and of the two techniques, ERIC-PCR was more informative. The diversity analysis provides a population structure of B. thuringiensis in India. Key words: Bacillus thuringiensis, Molecular typing, REP, ERIC-PCR, India