Browsing by Author "Hemalatha, Thiagarajan"
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ArticleItem Open Access Myocardial Expression of PDECGF is Associated with Extracellular Matrix Remodeling in Experimental Myocardial Infarction in Rats(Biochem, Cell Biol., 2010) Hemalatha, Thiagarajan; Balachandran, Chidambaram; Manohar, Bhakthavatsalam Murali; Nayeem, Mohammed; Subramaniam, Samu; Hari, S. Sharma; Puvanakrishnan, Rengarajulu; TANUVASPlatelet-derived endothelial cell growth factor (PDECGF) is a potent angiogenic peptide with anti-apoptotic activity expressed widely in tumours. However, its expression in myocardial infarction (MI) is not yet established. The study animed to assess the myocardial expression of PDECGF in rats after MI. Extracellular matrix (ECM) remodeling plays an important role in angiogenesis; hence, changes in the ECM components were investigated in the myocardium after MI, which was induced in rats by coronary artery ligation (CAL) and verified using biochemical markers and histopathology. Immunohistochemistry, RT-PCR, and activity assays identified the expression pattern of PDECGF on day 1,2,4,8,16, and 32 after CAL. The levels of TNF-α MMP-2, collagen, and glycosaminoglycans in the ECM were assessed. Studies on immunohistochemistry, RT-PCR, and PDECGF activity demonstrated elevated levels of PDECGF expression from day 2 after CAL. Macrophages, endothelial cells, fibroblasts, and cardiomyocytes, especially at the border region of the lesion, showed an enhanced expression for PDECGF. Remodeling of the ECM was depicted by changes in the levels of TNF-α, MMP-2, collagen, and GAG. Hence, this study clearly indicated PDECGF as an important angiogenic molecule expressed during MI Aand the alterations in ECM components facilitated the process of angiogenesis.ArticleItem Open Access Platelet-derived Endothelial Cell Growth Factor Mediates Angiogenesis and Antiapoptosis in Rat Aortic Endothelial Cells(Biochem, Cell Biol., 2009) Hemalatha, Thiagarajan; Tiwari, Mitali; Balachandran, Chidambaram; Manohar, Bhakthavatsalam Murali; Puvanakrishnan, Rengarajulu; TANUVASThis study explores the angiogenic and antiapoptotic activities of platelet-derived endothelial cell growth factor (PDECGF) in rat aortic endothelial cells. The effects of PDECGF on rat aortic endothelial cell (RAEC) proliferation, migration, chemotaxis, and tubule formation were investigated in vitro at various concentrations viz., 1,2,4,8,16, and 32 ng.mL-1 on endothelial cells. Endothelial cells were induced with hypoxic stress and the antiapoptotic effects of PDECGF were analysed by cell survival assay, fluorescence microscopy, cell viability assay, and flow cytometry. The results demonstrated the angiogenic potential of PDECGF on endothelial cells in a dose-dependent manner. PDECGF at 16 and 32 ng.mL-1 increased cell proliferation (>80%), induced cell migration (>4 fold), stimulated chemotaxis (>2 fold), and increased tubule formation (>3 fold) compared with the control. Studies on hypoxic stress revealed the antiapoptotic nature of PDECGF on endothelial cells. PDECGF on endothelial cells. PDECGF treatment enhanced cells survival by 14%, as well as cell viability by 13% and decreased the percentage of apoptotic cells by 13% as demonstrated by fluorescence-activated cell sorter studies (FACS). In conclusion, this study demonstrated the angiogenic and antiapoptotic potentials of PDECGF on RAEC.