Browsing by Author "Gurjot Kaur"
Now showing 1 - 2 of 2
Results Per Page
Sort Options
ThesisItem Open Access Design and Development of Software for the Implementation of Image Processing Approach for Leaf Area Measurement(Punjab Agricultural University, Ludhiana, 2014) Gurjot Kaur; Salam DinSeveral commercial softwares are available for plant leaf area measurement. But no freeware is available for leaf area calculation. This study proposes a freeware for calculating the plant leaf area. It is hypothesized that if the error between the calculated leaf area and the actual leaf area is low then it could be used for agronomical research. Leaves of Brassica species, turmeric, soybean and wheat with different size and shape are selected for the study. The platform used for image processing and leaf area calculation is DOTNET framework at the front end and SQL server at the back end. A digital scanner is utilized to obtain leaf images. The user can browse the scanned image of a leaf in any image format and the freeware automatically convert it into web compatible image format. For color tonal distribution the histogram of images is computed. The colored image is converted into monochrome image by setting the threshold value in the color matrix. In image processing method leaf area is calculated through pixel number statistic. The results are compared with the results of Leaf Area Meter by Disha Infoway India Private Limited and concluded that the error rate between them is less than ∓2% for Brassica species, turmeric, soybean and wheat leaves.ThesisItem Restricted Isolation and characterization of endophytic bacteria from rice (Oryza sativa L.)(Punjab Agricultural University, Ludhiana, 2016) Gurjot Kaur; Sharma, PoonamThe present investigation was carried out to exploit diverse endophytic bacteria from 19 recommended rice and basmati rice varieties. Total 43 endophytic bacteria were isolated from root and leaf tissues of rice. On the basis of morphological and biochemical tests 81.5%, 81.4%, 67.5%, 16.3% and 7% isolates were positive for oxidase, nitrate reduction, methyl red, citrate utilization and Voges Proskauer’s, respectively. All endophytic bacterial isolates were positive for catalase test. Of 43 isolates 60.5%, 23.3% and 16.3% were tentatively assigned as Bacillus sp, Pseudomonas sp and Klebsiella sp, respectively. Further, all isolates were screened for multiple Plant Growth Promoting (PGP) traits viz. phosphate (P) and zinc (Zn) solubilization, production of indole acetic acid (IAA), 1-Aminocyclopropane-1-carboxylate (ACC) deaminase, siderophore, cell wall degrading enzymes, HCN, fluorescent pigment, urease and denitrification activity. Out of 43 endophytic bacterial isolates, 65.1%, 30.2%, 25.6% 18.6%, 18.6%, 16.3% and 4.6% produced fluorescent pigment, protease, denitrification, urease, siderophore, cellulase and zinc solubilization, respectively. Significantly high amount of IAA production was recorded with LRBLE1 (19.6 µgml-1) isolated from basmati rice variety Pusa 1592 followed by LRBLE4 (18.8 µgml-1) isolated from basmati rice variety PB 2. Significantly high P solubilization was observed in LRBLE1 (16.4 mg100ml-1) followed by LRBLE 4 (8.3 mg100ml-1). Highest ACC deaminase production was recorded with LRBLE1 (OD 0.555nm) followed by LRBLE 4 (OD 0.496nm). On the basis of above PGP traits 9 isolates (LRRE1, LRRE13, LRRE18, LRLE6, LRBRE4, LRBRE6, LRBLE1, LRBLE4 and LRBLE7) were subjected for colonization study using ampicillin (1 μgml-1) an antibiotic marker. Isolate LRRE1 (3.43) showed maximum log CFU/g root fresh weight. Further, on the basis of molecular characterization 3 endophytic bacterial isolates (LRBLE1, LRBLE4 and LRBLE7) were identified as diazotrophs due to presence of nifH gene. Six isolates (LRBLE4, LRBRE6, LRLE6, LRBRE4, LRBLE7 and LRRRE13) were detected as Pseudomonas sp with genus specific primer. On the basis of PGP traits and molecular characterization LRBLE1 and LRBLE4 were found promising diazotroph endophytic bacteria in rice.