Browsing by Author "Gupta, M. P."
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ThesisItem Open Access Demand And Supply Of Eggs In Ludhiana ( Punjab ).(IARI, Division Of Agricultural Economics ; New Delhi, 1970) Sidhu, D. S.; Gupta, M. P.ThesisItem Open Access Economic Analysis Of Grower's Processor Contract And Relationship For Green-Peas In Contract Farming Areas Of Uttar Pradesh(IARI, Division Of Agricultural Economics ; New Delhi, 1968) Sharma, Surendra Nath; Gupta, M. P.ArticleItem Open Access Effect of dansyl chloride on the rennet susceptibility of the casein micellesGupta, M. P.; Ganguli, N. C.ArticleItem Open Access Effect of EDTA treatment on the stability of the buffalo casein micellesGupta, M. P.; Ganguli, N. C.ArticleItem Open Access Effect of ions on the stability of casein micellesGupta, M. P.; Ganguli, N. C.ArticleItem Open Access Effect of ultra centrifugal whey on the release of components from buffalo casein micelles(1980) Gupta, M. P.; Ganguli, N. C.ArticleItem Open Access Electrophoretic and gel filtration studies of rennet susceptibility of buffalo casein micellesGupta, M. P.; Ganguli, N. C.ThesisItem Open Access Epidemiological, Haematobiochemical Observations And Chemotherapy Of Paramphistomosis In Buffaloes (Bubalus Bubalis)(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 2003) Kumar, Sunil; Gupta, M. P.The present study was carried out to assess the Epidemiological,Haematobiochemical observations and efficacy of Oxyclozanide and Triclabendazole against Paramphistomosis in Buffaloes (Bubalus bubalis) in bet area in and around Ludhiana district and total of 500 random buffalo faecal samples were collected. The overall coprological prevalence of paramphistomosis was found to be 23%. The haematological profiles of buffaloes with paramphistomosis revealed anemia, leukocytosis, neutrophilia and eosinophilia as compared to healthy control animals. Serum protein profiles of infected buffaloes showed hypoproteinemia, hypoalbuminemia and decreased A/G ratio. Mineral profiles reflected significant reduction in iron concentration in infected buffaloes. The eggs per gram(EPG) were elevated in infected buffaloes and found to be 770±110.5(Infected and Treated Group C) and 680±170.5 (Infected and Treated Group D). However, after treatment with oxyclozanide @ 1 gm/100 Kg bwt (orally) and triclabendazole @l0m1/50 Kg bwt (orally), the infected buffaloes showed in recovery clinical signs and their haematological and biochemical profiles improved. On the basis of reduction eggs per gram on successive days of observation, it was concluded that oxyclozanide was much more (100% at day 28th after treatment) efficient against paramphistomes in buffaleos than triclabendazole, which showed very little efficacy (55.8%) against paramphi stomes.ThesisItem Open Access Epidemiology And Chemotherapy Of Parasitic Diseases In Zoo Animals Of M.C. Zoological Park, Chhatbir, Punjab(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 2004) Singh, Parvesh; Gupta, M. P.The present investigation was undertaken to study the epidemiology and chemotherapy of parasitic diseases in zoo animals at M.0 Zoological Park. Chhatbir used to determine the prevalence and intensity of endoparasitic load in zoo animals. The overall prevalence of endoparasitic infections in the zoo animals was found to be 33.22 per cent with maximum prevalence in carnivores (58.68 %) followed by omnivores (29.02 %) and herbivores (25.71 %). The incidence of haemoprotozoan infections in carnivores viz lions and tigers was 2.85 per cent. The haemoprotozoan parasite reported was Babesia spp in lions. Nematodes were the most common type of parasites detected followed by coccidian parasites, cestodes and trematodes. Highest prevalence of endoparasitic infection (nematodes) especially ascarids was reported in carnivores. The intensity of endoparasitic infection as well as the mean cpg values varied a great deal in various species of animals with epg values ranging between 50-8800 for various parasites. The parasitic infections wee detected in 12 per cent of the animals died at the zoo which included Tavocara cati, Toxascaris leonina and Anoplocephela magna. The various parasites collected during investigation were identified based on their morphological characteristics by gross, microscopic and scanning electron microscopic (SEM) studies. It was found that treatment of animals with appropriate drug based on the species of parasites present in these animals was reported to be 100 per cent effective in majority of cases based on reduction in faecal egg count which reached to zero by day 5 post treatment while in some groups it was reached by day 7 and 15 post treatment and there was no reoccurrence of parasitic infection till day 55 post treatment except for lions in which there was reoccurrence of Toxascaris leonina infection by day 30 post treatment. Keywords: M.C.Zoological Park, Prevalence, Tavocara Toxascaris leonhia. Anoplocephela magna, Babesia spp, Scanning electron microscopy, Treatment.ThesisItem Open Access Haemato-biochemical and therapeutic studies on ancylostomosis in dogs(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 2016) De, Subhra; Gupta, M. P.Ancylostomosis is an important wide spread highly pathogenic metazoan nematodal parasitic infection of dogs and related carnivores which is an important zoonoses as it causes cutaneous larva migrans in humans. During the present study coproscopic examination of 250 dogs done in the laboratory by flotation technique of dogs presented in the GADVASU Small Animal Clinic, Ludhiana, Punjab from March 2015 to February 2016 and revealed the prevalence of ancylostomosis to be 13.20 per cent. The eggs per gram (epg) of faeces ranged from 600-28,300 as conducted by Mc master counting technique. Micrometry revealed the mean size of eggs were, length- 65.75 µm and the breath- 43.22 µm, means the species prevalent here in ludhiana is Ancylostoma caninum. The monthly prevalence of ancylostomosis from March to February varied from 5.55 to 26.66 per cent. The highest prevalence in the month of October and lowest in March. The values of the prevalence were correlated with the meteorological data. Highest prevalence was at the mean temperature of 25.10c and humidity 68 per cent which was seen in the month of October. The season-wise highest prevalence was in the season of Autumn and the lowest prevalence was in the month of winter. The prevalence was higher in male (14.37%) dogs as compared to their female counterparts (10.84%). The breed wise highest prevalence was seen in Mongrel 19.23%, followed by German sheperd 13.79%, Pomeranian 13.33% Pug 8.33 and in other breeds 6.89 per cent. The prevalence was higher in 0-6 months old pups (28%) than adults. The main clinical signs recorded were reduced body weight, anaemia as recorded by slight pale to pale mucous membrane, anorexia, pica, black color soft faces, vomition and diarrhoea. Four groups were made from naturally infected dogs came to the small animal Veterinary Medicine Clinic, GADVASU, Ludhiana. Group I (Healthy control), Group II (Non-treated control), Group III (Treated with ivermectin) and Group IV (Treated with pyrantel pamoate) were made. Group I, Group III, Group IV were consisted of 10 animals each and Group II consisted of 6 animals. The blood sample collected for Hemato-biochemical study on 0, 7th and 15th day. The study revealed decrease in Hb, PCV, TEC, hypoalbuminaemia, hypoprotienaemia and lower serum iron in ancylostomosis infected dogs. There is slight decrease in MCH, MCV and MCHC was observed in comparing with the healthy control. There was increase in TLC, N and E. In the treated groups after the institution of therapy there was some improvement in Hb, PCV, TEC, TPP, A, Serum iron. Eggs per gram (EPG) were performed before and on day 7th and day 15th post-treatment (DPT). Ivermectin and pyrantel pamoate was given as single dose administration @ 0.2 mg/kg S/C and 5 mg/kg per oral respectively. Examination before and after treatment revealed that the dogs which were naturally infected with A. caninum, based on EPG values, the efficacy were 100% against A. caninum following treatment with ivermectin and pyrantel pamoate on day 7 post treatment (PT) based on average reduction in EPG. On 15th day in pyrantel pamoate treated group, the mean EPG became 30 ± 30. So, pyrantel pamoate is marginally less effective than ivermectinThesisItem Open Access Serological and molecular diagnosis of brucellosis and impact of managemental practices on its control in cattle and buffaloes(2012-06-15) Islam, Malik Raies Ul; Gupta, M. P.During the present study a total of 2345 whole blood samples (1730 cattle and 615 buffaloes) and 9 aborted material samples were collected from the selected animals. All the samples were screened using Rose Bengal Plate Test (RBPT). An overall seroprevalence of 20.89% was observed. Among the three agro-climatic zones highest prevalence was observed in central zones (22.62%). Cattle were found to be more susceptible to infection compared to buffaloes (p ≤ 0.01). A battery of four serological tests viz Rose Bengal Plate Test (RBPT), indirect Enzyme linked immunosorbent assay (I-ELISA), Micro-agglutination test (MAT) and modified Micro-agglutination (mMAT) were evaluated on 900 serum samples. A total of 260 samples were found positive by RBPT, 352 samples were positive by I-ELISA, 281 by MAT and 262 by mMAT. A substantial degree of agreement was observed between I-ELISA and rest of the tests. Fifty Brucella positive animals were followed for four months and samples were collected at monthly intervals. The titre end points were determined using MAT. Some of the animals were having low titres at the beginning, which rose over the period of time, while others had very high titres which declined with time. PCR detected amplicons of 193-bp in 68 sera and 6 samples from aborted foetuses and a least degree of agreement was observed between IELISA and PCR. All the 6 positive aborted foetal materials were identified to be positive for B. abortus. PCR using DNA from six B. abortus strains amplified five fragments of 1682, 794, 587, 450 and 152 bp in size. PCR with B. abortus S19 DNA did not produce 587bp fragment common to Brucella strains tested. For risk factor identification data from 39 dairy farms were collected analysed, and factors which increased the risk of an animal being infected with B. abortus included, no precaution taken regarding visitors, no disinfection of farm premises, female introduction without testing, farm replacement, breeding method, serological testing, attitude with positive animals, culling of affected animals, knowledge of farmer about brucellosis and herd size (p < 0.05). For observing the impact of managemental practices on control of brucellosis ten dairy farmers were selected. Out of these ten farmers 9 could bring down the prevalence of the disease to a very low level. From the results it is evident that educating the farmers may prove very fruitful for combating this disease which has assumed alarming prevalence in India in general and Punjab in particular.ThesisItem Open Access Studies on epidemiology, blood coagulation, immuno-biochemical alterations and mastitis therapy in bovines(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 2008) Renu; Gupta, M. P.There are a number of factors associated with incidence of mastitis like environmental, genetic and nutritional. The present investigations were aimed at studying the risk factors associated with mastitis in cattle and buffalo, to study tie alterations in blood coagulation, biochemical and immunological parameters in animals suffering from mastitis and to study the effect of treatment on these parameter; in animals suffering from mastitis. The prevalence of mastitis was found to be in the range of 2.38 to 3.46 %. Streptococcus spp was found to be causing majority of i ifections followed by Staphylococcus aureus and E.coli bacteria. En-ofloxacin was found to be most effective antibiotic whereas most of the strains were resistant to Penicillin. No significant difference between affected and healthy animals in their hematolotogical parameters was seen. There was no significant difference between affected and healthy animals for parameters related to blood coagualation. There was significant decline in the serum P-carotene concentration in affected animals (237 ± 40.63 1.1g/dI vs 408.08 ± 27.41 pg/d1) and the administration of Vitamin A to the mastitic animals led to a significant improvement in the condition. There was no significant difference in the level of Plasma ascorbate in mastitic (2.32 ± 0.45 mg/di) and non mastitic animals (2.50 ± 0.51 mg/di). Blood Zn concentration was found to be significantly lower in mastitic animal, whereas no difference in mean blood Cu and Co concentration was observed. There was significant rise in SCC and pH of milk in animals affected with clinical mastitis. There was found to be 5ignificant rise in LDH activity in milk from affected animals (958.96 ± 341.03 U/L vs 247.06 ± 89.47 U/L). The levels of milk LDH were observed to be good indicators of severity of mastitis and may be recommended as adjunct to diagnosis of mastitis. The level of citric acid mastitic milk was significantly lower (0.42 ± 0.29 mg/ml Ns 2.12 ± 0.35 mg/ml) and the administration of citrate to the mastitic animals helped in improving the clinical condition of the animals. Milk and serum immunoglobu dins concentration was significantly higher in mastitic animals (15.27 ± 3.58 and 30.63 ± 1.89 mg/m1 vs 6.42 ± 0.42 and 26.50 ± 1.47 mg/ml). A negative correlation between SCC and Lysozyme and Myeloperoxidase production and a positive correlation wiih Hydrogen peroxide and superoxide production was found.ThesisItem Open Access Studies On Epidemiology, Prevention And Treatment Of Mastitis With Non-Antibiotic Therapy In Cows(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 2005) Seema; Gupta, M. P.The study was carried out in a herd of 80 animals during period from March 2004 to April 2005. Incidence rate of mastitis was 36.14 per cent. The symptoms varied from inflammatory swelling of mammary gland or fibrosis of mammary gland, secretion of either thicker or thinner consistency of milk, presence of flakes, pus and blood clots in milk. Mean value of pH of milk sample f rom diseased animals ranged from 6.41 ± 0.578 to 8.416 ± 0.622. Mean value of somatic cell count in affected animals ranged from 14.25 ± 9.49 to 35.5 ± 20.07 lac cells/ml of milk and 1.67 ± 1.67 to 6.56 ± 3.12 lac cells/ml of milk during pre treatment and post treatment period respectively. Vitamin C level was found to be decreased in animals suffering form mastitis viz. from 1.0081 ± 0.307 to 1.0215 ± 0.0127 mg/di from normal level of vitamin C 1.096 ± 0.11 to 1.0988 ± 0.0134 mg/di estimated in healthy animal i.e. decreased by 8 per cent. There was significant reduction in vitamin E level during pre treatment period in mastitis affected animal i.e. 3.303 ±0.0384 to 3.445 ± 0.1899 vtrno1/1 from normal level of vitamin E i.e. 4.341 ± 0.216 to 4.365 ± 0.239 estimated in healthy animal i.e. decreased by 28 per cent. There was significant reduction in selenium i.e. 0.303 ± 0.90 to 0.311 ± 0.097 ug/m1 in infected animals from normal healthy animals i.e. 0.751 ± 0.056 to 0.786 ± 0.056 vtg/m1 i.e. decreased by 59 per cent. Vitamin C deficient animals were treated with injection redoxin 20m1 i/m for five days and they showed restoration of normal value. Vitamin E deficient animals were treated with vitamin E capsules, vitamin E care Se injection and injection vetade separately and animal showed improvement and they restored normal value. The recovery rate was 50 per cent, 66.66 per cent, 66.66 per cent, 80.33 per cent and 100 per cent in vitamin C treated, vitamin E capsule and vitamin E care Se injection treated, vetade treated, antibiotic treated and antibiotic in combination with vitamin E care Se injection treated animals respectively. The organisms isolated were mostly Gram -ve bacilli. In some animals Gram +ve cocci were also isolated. Pefloxacin was found the most sensitive drug during culture sensitive test. The samples have been collected from buffaloes suffering from mastitis and were treated similarly successfully.ThesisItem Open Access Studies on serological response in female calves vaccinated with S-19 Brucella abortus vaccine and molecular diagnosis of brucellosis in cows and buffaloes(GADVASU, Ludhiana, 2010) Fareed, Aadil; Gupta, M. P.Brucellosis is an infectious disease caused by Gram negative facultative intracellular bacterial organisms of the genus Brucella. The total of 479 blood samples were collected to determine the seroprevalence of Brucellosis among 3 different agroclimatic zones of Punjab using Brucella abortus coloured antigen (RBPT). Percent seroprevalence in submontane zone (Zone I), central zone (Zone II) and arid irrigation zone (Zone III) was found to be 17.74, 22.91 and 6.17 respectively The antibodies against Brucella abortus were found to be in 57 out of 264 cattle (21.5%) and 36 out of 215 buffaloes (16.7%). The overall seroprevalence of Brucellosis using RBPT was found to be 19.41%. The incidence of abortion was significantly more (90.32%) in last trimester of pregnancy, while history of repeat breeding was also found to be in 16.39% of Brucella positive animals. 19 female calves vaccinated with Brucella abortus S-19 calfhood vaccine were found to show a serological response of first increase in the antibody titre upto 60th day post-vaccination and then decrease in the titre thereafter using serological tests of STAT and MTPAT. In the second group of 15 adult cows already vaccinated with Brucella abortus S-19 vaccine were found to show negative titre on application of RBPT, STAT and MTPAT after a lapse of 18 months post-vaccination. Out of 40 samples of aborted fetal stomach/abomasal contents and/or vaginal mucus/uterine discharges of recently aborted animals, 11 were found to be positive for Brucellosis on application of molecular diagnostic test of PCR. Out of 20 seropositive heparinised whole blood samples, 4 were found to be positive for Brucellosis on applying PCR.ThesisItem Open Access Studies on the Formation Constants of binary and Ternary Complexes of Some Transition metal Ions(College of Basic Sciences and Humanities Chaudhary Charan Singh Haryana Agricultural University Hisar, 1991) Shish Ram; Gupta, M. P.ThesisItem Open Access Studies On Therapeutic Efficacy And Disposition Pattern Of Antibiotics In Clinical Cases Of Mastitis In Buffaloes(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 1999) Kaur, Kiran Deep; Gupta, M. P.ThesisItem Open Access Wastelands of Hisar District Physico Chemical Properties of Soils and Evaluation(College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 1991) Jain, Praveen Kumar; Gupta, M. P.